LOCUS       sequence-27289-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: GFP-D1a_W2-3(aa318-399)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    51..75
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(351..375)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    412..546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(436..455)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     490..509
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(662..678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(662..678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(675..697)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    686..702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(710..716,717..734,735..740))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    755..775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cacgggatcc tctcggcatg gacgagctgt acaagtctag aggatcatca acaagtttgt
       61 acaaaaaagc aggctttgga gccataataa gcacatgtca ggtctctgtg gatgctcctg
      121 aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta agttcttcat
      181 caaatggagg gttaaatcca agcactttga attcagctag caaccatggt gcctggccag
      241 tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt tctggcatta
      301 atattcagtg cagtactata ggccagatgc ctaacaatca gagtatttga acccagcttt
      361 cttgtacaaa gtggttgatt cgacctcgag gccttaagtg actaggtacc cgatccagac
      421 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      481 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      541 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      601 gttttttaaa gcaagtaaaa cccaagctgg gaagctcaga tctgagcttg gcgtaatcat
      661 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      721 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acatttagtt
      781 gcgttgcgct cactgcccgc tttccagtcg gcacctgctc gtgtcgcagc tgcattaatg
      841 aatcggccac cgccgcgggg aagaggcggt ttgcgtattg
//
