LOCUS       sequence-272895-        8711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mRuby2-tagged Lifeact with Puromycin 
            selection in cells including neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8711)
  TITLE     Lentiviral Lifeact
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti Lifeact-mRuby2 PuroR
FEATURES             Location/Qualifiers
     source          1..8711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(268..273,274..291,292..298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     365..385
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        367..385
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        413..639
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             640..820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    867..992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1485..1718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1903..1947
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2214..2330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2387..2411
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2527..2730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2681..2701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2727..2751
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2807..2816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2813..2863
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      2879..2888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2885..3595
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     protein_bind    3625..3645
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(3757..3781)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3812..4400
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3865..3885)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4283..4294)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4414..4913
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4449..4472)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4846..4865
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4948..5547
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4948..4967)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5444..5464
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5642..5875
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5947..6068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5984..6003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6038..6057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6108..6243
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6263..6282)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6264..6282)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6292..6309)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6292..6308)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6301..6323)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6450..6905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6537..6556)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6747..6768
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6931..7035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7036..7104,7105..7896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7254..7273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8067..8655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8556..8575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgtccagt
     2341 gtggtggaat tgagtattcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
     2401 aaaagcaggc tggagaccca agcttggtac cgagctcgga tccactagta ttatgcccag
     2461 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2521 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2581 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2641 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2701 gtacggtggg aggtttatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2761 cgccatccac gctgttttga cctccataga agattctaga gctagcgcca ccatgggcgt
     2821 ggccgacttg atcaagaagt tcgagtccat ctccaaggag gagggggatc caccggtcgc
     2881 caccatggtg tctaagggcg aagagctgat caaggaaaat atgcgtatga aggtggtcat
     2941 ggaaggttcg gtcaacggcc accaattcaa atgcacaggt gaaggagaag gcaatccgta
     3001 catgggaact caaaccatga ggatcaaagt catcgaggga ggacccctgc catttgcctt
     3061 tgacattctt gccacgtcgt tcatgtatgg cagccgtact tttatcaagt acccgaaagg
     3121 cattcctgat ttctttaaac agtcctttcc tgagggtttt acttgggaaa gagttacgag
     3181 atacgaagat ggtggagtcg tcaccgtcat gcaggacacc agccttgagg atggctgtct
     3241 cgtttaccac gtccaagtca gaggggtaaa ctttccctcc aatggtcccg tgatgcagaa
     3301 gaagaccaag ggttgggagc ctaatacaga gatgatgtat ccagcagatg gtggtctgag
     3361 gggatacact catatggcac tgaaagttga tggtggtggc catctgtctt gctctttcgt
     3421 aacaacttac aggtcaaaaa agaccgtcgg gaacatcaag atgcccggta tccatgccgt
     3481 tgatcaccgc ctggaaaggt tagaggaaag tgacaatgaa atgttcgtag tacaacgcga
     3541 acacgcagtt gccaagttcg ccgggcttgg tggtgggatg gacgagctgt acaagtaagc
     3601 ggcctcgagc atgcatctag aggacaactt tgtatacaaa agttgggaga cccaagcttg
     3661 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattctg cagatatcca
     3721 tcacactggc ggccgctcga gcatgcatct agaggtaccc agctttcttg tacaaagtgg
     3781 ttgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga
     3841 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3901 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3961 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4021 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4081 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4141 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4201 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4261 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4321 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4381 cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt ttcccaaggc
     4441 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4501 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     4561 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     4621 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     4681 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     4741 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4801 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     4861 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     4921 gcccaagctt accacactcc tgcaggcatg accgagtaca agcccacggt gcgcctcgcc
     4981 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5041 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5101 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5161 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5221 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5281 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5341 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     5401 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5461 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5521 tgcatgaccc gcaagcccgg tgcctgaacg cgtatgcatc tcgagggccc ggtaccttta
     5581 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5641 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5701 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5761 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5821 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5881 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5941 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6061 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6121 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6181 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6241 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6301 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6361 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6421 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6481 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6541 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6601 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6661 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6721 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6781 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6841 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6901 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6961 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7021 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7081 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7141 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7201 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7261 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7321 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7381 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7441 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7501 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7561 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7621 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7681 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7741 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7801 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7861 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8701 ccttttgctc a
//
