LOCUS       sequence-272899-        3756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr160.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr160
FEATURES             Location/Qualifiers
     source          1..3756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    519..540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(555..560,561..578,579..585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     598..620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     617..633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     617..633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        654..672
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(1338..1349)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(1598..1617)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1599..1617)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1624..1641)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1624..1640)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1633..1655)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1782..2237
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1869..1888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2079..2100
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2263..2367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2368..2436,2437..3228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2586..2605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3399..3756,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac
      661 cctcactaaa gggaacaaaa gctggagctc cagctacccc tcttcctcct ctttctgggg
      721 atctacgtag tcaccatggt aggaaacctg ggcatgatca ccttgattgg actcaactca
      781 cagcttcaca cccccatgta cttcttcctc agtaacctgt cactcgtgga tctctgctac
      841 tcctctgtca ttacccctaa aatgctcatc aactttgtgt cccaaagaaa cctcatctcc
      901 tatgtgggat gcatgtctca gctatacttc ttcctggttt tcgtcattgc tgagtgttac
      961 atgctcactg tgatggccta tgaccgctat gtggccatct gtcaaccctt gctttataac
     1021 atcatcatgt ctcctgccct ctgctctctg ctggtggctt ttgtctatgc cgtaggactc
     1081 atcggttcag caattgagac tggcctcatg ttgaaattaa actattgtga ggacctcatc
     1141 agccactact tctgtgacat cctccccctc atgaagctct cttgctctag tacctatgat
     1201 gtagaaatgg ctgtcttctt tttagctgga tttgacatta tcgtcacaag cttaaccgta
     1261 ctcatttcct atgcattcat cctgtccagc atccttcgca tcagctccaa tgagggcagg
     1321 tccaaagcct tcagcacctg cagctcccac tttgcagctg tgggcttgtt ctatggatcc
     1381 acagcattca tgtacttaaa gccttctaca gctagttccc tggcccagga gaatgtagct
     1441 tctgtgttct acaccacagt gatccccatg ttcaaccccc tgatctacag cctgagaaac
     1501 aaggaggtga agaccgcact ggacaaaaca ctgaggagaa aagtcttttg atgtttaatt
     1561 aagcggccgc gtttaaacac gcgtggtacc caattcgccc tatagtgagt cgtattacaa
     1621 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     1681 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     1741 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     1801 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     1861 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1921 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1981 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     2041 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     2101 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     2161 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     2221 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2281 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2341 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2401 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2461 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2521 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2581 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2641 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2701 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2761 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2821 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2881 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2941 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3001 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3061 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3121 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3181 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3241 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3541 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3721 gggggttcgt gcacacagcc cagcttggag cgaacg
//
