LOCUS       sequence-27290-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: GFP-D1a_W3-4(aa340-439)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..74
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(401..425)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    462..596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(486..505)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     540..559
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(712..728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(712..728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(725..747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    736..752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agcgggatct ctcggcatgg acgagctgta caagtctaga ggatcatcaa caagtttgta
       61 caaaaaagca ggctcttcat caaatggagg gttaaatcca agcactttga attcagctag
      121 caaccatggt gcctggccag tattagagaa caatggactt gccctaaaag ggcctgtagg
      181 gagtggtagt tctggcatta atattcagtg cagtactata ggccagatgc ctaacaatca
      241 gagtattaac tctaaagtga gtggtggttc tacccatggt acctggggaa gccttcagga
      301 aacttgtgaa tctgaagtaa gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa
      361 tattaccact gaaatgactg gaccaaataa cactatctga acccagcttt cttgtacaaa
      421 gtggttgatt cgacctcgag gccttaagtg actaggtacc cgatccagac atgataagat
      481 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      541 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      601 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
      661 gcaagtaaaa cccaagctgg gaagctcaga tctgagcttg gcgtaatcat ggtcatagct
      721 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggagagtg
      781 aaagtggtaa agcctggggg
//
