LOCUS       sequence-272903-        3965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr17
FEATURES             Location/Qualifiers
     source          1..3965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1283..1292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1287..1296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1326..1345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1327..1345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1352..1369)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1352..1368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1361..1383)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1509..1964
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1596..1615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1806..1827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2042..2146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2147..2215,2216..3007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2365..2384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3178..3766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3667..3686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3920..3937
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa
      241 tactcaagct atgcatccaa cgcgttggga gctctcccat atggtcgacc tgcaggcggc
      301 cgcgaattca ctagtgatta tgacctgggg aaactggaca actgtcaggg aatttatcct
      361 catgagcttc tcaagcttgt cctatgaagt acaggctctg ctatttctcc tgtttttggt
      421 catttaccta gtcacactca tgggcaatgt cctcatcatc ctggttacta cagctgactc
      481 tgctctgcaa agtcctatgt acttcttcct cagaaactta tccttcctgg aaataggctt
      541 caacttggtc attgtgccca agatgttgag tactctgata ctccaggaca aaaccatctc
      601 cttccttggc tgtgctactc agatgtattt cttctttttc tttggagctg ctgagtgctg
      661 cctcctggcc acaatggcat atgaccgcta catggcgatc tgtgatccct tgcactaccc
      721 aataatcatg agccgaagat cctgtgccca gctagcagct gcctcttggt tctccgggtt
      781 cccagtagcc actgtgcaaa ccacatggat tttcagtttc cctttttgtg gcccaaacat
      841 ggtgaaccac ttcttctgtg acagcccacc tgtcatagcc ctagtctgtg ctgatacatc
      901 cctatttgaa ctggaggctc taacagccac tgtcctattc atcctctttc ctttcttgtt
      961 gattctgggt tcctatgtcc gcatcctctc taccatcttt aggatgcctt cagctgaagg
     1021 gaaacgcaaa gccttttcca cttgctcctc ccacctcctc gttgtctccc tcttctacag
     1081 cactgccatc ctcacatact tccggccacg ctcaaacacc tcccctgaga acaagaaaat
     1141 gttgtcactc tcctacacag ttgtcactcc catgttgaac cccatcatct acagcttaag
     1201 gaataatgag gtgaaggctg cattgaggcg aatcattcac agaactctgg gccctcagaa
     1261 actatgaaat cgaattcccg cggccgccat ggcggccggg agcatgcgac gtcgggccca
     1321 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     1381 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1441 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1501 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1561 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1621 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1681 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     1741 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     1801 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     1861 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     1921 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     1981 ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt
     2041 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2101 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2161 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2221 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2281 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2341 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2401 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2461 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2521 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2581 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2641 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2701 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2761 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2821 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2881 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2941 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3001 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3061 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3121 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3181 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3241 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3301 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3361 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3421 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3481 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3541 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3601 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3661 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3721 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3781 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3841 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3901 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3961 caaac
//
