LOCUS       sequence-27291-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: GFP-D1a_W4-5(aa409-495)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(358..382)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    419..553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(443..462)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     497..516
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(669..685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(682..704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    693..709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(717..723,724..741,742..747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tcgggatctc tcggcatgga cgagctgtac aagtctagag gatcatcaac aagtttgtac
       61 aaaaaagcag gctctaaagt gagtggtggt tctacccatg gtacctgggg aagccttcag
      121 gaaacttgtg aatctgaagt aagtggtaca cagaaggttt cattcagtgg tcaacctcaa
      181 aatattacca ctgaaatgac tggaccaaat aacactacta actttatgac ctctagttta
      241 ccaaactccg gttcagtgca gaataatgag ctgcctagta gtaacacagg ggcctggcgt
      301 gtgagcacaa tgaatcatcc tcagatgcag gctccatcag gtatgaatgg cacttgaacc
      361 cagctttctt gtacaaagtg gttgattcga cctcgaggcc ttaagtgact aggtacccga
      421 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      481 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      541 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
      601 gtgggaggtt ttttaaagca agtaaaaccc aagctgggaa gctcagatct gagcttggcg
      661 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcat aagcgagtgg gctaaactca
      781 catatatttg cgttggcgct
//
