LOCUS       sequence-272922-        6022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Kao SC, Wu H, Xie J, Chang CP, Ranish JA, Graef IA, Crabtree GR
  TITLE     Calcineurin/NFAT signaling is required for neuregulin-regulated 
            Schwann cell differentiation.
  JOURNAL   Science. 2009 Jan 30. 323(5914):651-4.
  PUBMED    19179536
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Jan 30. 323(5914):651-4."
COMMENT     Sequence Label: MPZ(P0)promoter pGL3-TATA
FEATURES             Location/Qualifiers
     source          1..6022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1243..1252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1249..2901
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1315..1333)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2942..3063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2953..2972)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3007..3026
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3311..3328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3482..4070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3562..3581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4241..5032,5033..5101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4864..4883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5102..5206)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5233..5688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5320..5339)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5530..5551
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5819..5867
                     /note="synthetic polyadenylation signal"
     misc_feature    5881..5972
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5921..5940
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tcacacttcc tgttcagact tctttcctag tgaacccttt caagagcccc cacaccgaat
       61 caaacatgga tggcacaaac atggatgctc taaatctaca cagagcttca caaatgtcac
      121 acgtgcacac actttcacct cagctcttac ctttgctgtt ccatctagcc attgcccctc
      181 ccgttccctt tttcaaaatg gctgctcctt caaggtctct agacaacact gcttcctaga
      241 cctgattcct ctttcctctg aacttcctgt attaagtggc attcccaccg ttctgtgcat
      301 tggcagttga tgagtttgcc ctctgtttct cccctctgcc tcctccaact agatcttgag
      361 cttgtagaaa gaactgaatt accattctaa tgtgagcatt cgcattcccc aacgtttcaa
      421 atagccacca ggcaggccaa tacgcagtct tgatcattta aactgctgca ttgcataagg
      481 taccctaaca gacgggagaa atctgggctt cttcctggaa gtttcctggt agagaaaatc
      541 ttctatctgg gtagaatcta ccagatacag ggaggtggaa aaagttgttc ccagagggct
      601 ttatagtcta cagtgttgcc gtagcaatca gaacaacaga caccctaatt tgggagtgct
      661 ctgaaagaaa cttgcctcta ggtcctaggg ctctcaggca aggagaccaa gaaggattcc
      721 tttgccatgg ccttttggat ttaggtttct cagcttgtct attcctcaga gaagtgtgtc
      781 tatgcccctt ttctgtccct ctgcctcacc ctaccccaac attccaacct agggtagggg
      841 gaggtcagta tacacaaagc cctctgtgta aggggtggta tgtgtccccc cacccctcca
      901 cccagcgtat acaatgcccc ttctgctcca tgccccctgc caccctcccc accacctctc
      961 cattgcacat gccaggctgc aattggtcac tggctcagga cagccccctc atgctgggga
     1021 tccaggggat tttaagcagg ttccagaaaa cacagctcag ttccttgtcc cccgctctct
     1081 ccaccccaca gacactctgg gcctttgccc taccccagct atgggctagc ccgggctcga
     1141 gatctgcgat ctaagtaagc ttctcgagcg ggtctccgtg acgactataa aagcccaggg
     1201 gcaagcggtc cggaagcttg gcattccggt actgttggta aagccaccat ggaagacgcc
     1261 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     1321 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     1381 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     1441 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     1501 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     1561 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     1621 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     1681 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     1741 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     1801 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     1861 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     1921 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     1981 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     2041 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     2101 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     2161 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     2221 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     2281 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     2341 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     2401 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     2461 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     2521 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     2581 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     2641 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     2701 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     2761 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     2821 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     2881 ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg
     2941 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3001 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3061 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3121 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat
     3181 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     3241 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     3301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5221 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5281 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5341 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5401 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5461 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5521 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5581 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5641 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttgc cattcgccat
     5701 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     5761 ccaagctacc atgataagta agtaatatta aggtacggga ggtacttgga gcggccgcaa
     5821 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     5881 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     5941 cagtgcaagt gcaggtgcca gaacatttct ctatcgatag gtaccgagct cttcaccctc
     6001 ttcagtcgat catctgagac ct
//
