LOCUS       sequence-272923-       10971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-C13 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 10971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pC13N-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..10971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(976..1009)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1010..1035
                     /label=SA
                     /note="splice acceptor site"
     CDS             1059..1112
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1107..1907
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1167..1186)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1777..1796
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1942..2166
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(2167..2200)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3444..3477)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     enhancer        3548..3851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3853..4130
                     /label=chicken beta-actin promoter
     intron          4131..5147
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5071..5093
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5155..5174
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5235..5897
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     primer_bind     complement(6059..6078)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6124..6179
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6178..6197)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    6512..6545
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(8601..8618)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8601..8617)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8610..8632)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8826..8845)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8945..8967
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9098..9117)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9109..9918
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9176..9195)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10096..10684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10585..10604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10838..10855
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccgcg gccgcagcag gagaatcatg ccaatgggcc aatatacatt ctgacccaca
      181 gtttcataat aaaataaaat ggttgtggtt gtaagccact atgtttcaga gtggtttgtt
      241 acacagcaat aaataactaa tatagtaggc ataccatcaa gtccaaagta ggtagagaag
      301 aatgtaaata gcagagcaaa acagcatgac tggtggctgg gaggcttaaa actgggacag
      361 gatcagagtc atgaaagaag tcaaagaaat ggttcagaag taaggctgag actgacttac
      421 aaaagctgaa agtcccttta agttggtgtt tggtgcattg gcaggggcag gtatggtgac
      481 ttaaaagagc catgctcaac aagatcaagc acaacacaat cacgggtcac cccagcagac
      541 cttagcgagt ctagccattt ctttggtggt ggtcacagtc atgcttcagc ccagtttcca
      601 cttggacaaa tggtacatat tttcaatgag atgaaaatta agatacaatc catgtgctca
      661 gagagtgatc acagctctga ctaaacactg tgccccaaag tgttgaggaa ttgggaaaac
      721 ctagctgagt tagtggtctc ttttctgtta caataaagct cataatgaaa attagccttc
      781 tttgttcttc cccaagtctt tctttctaga cgaaactact ttcaactgtt ttaacttcct
      841 tactgttaac ttccatattt ctagatagta tggtcagact gttatttctt gacttttaaa
      901 tgtaagatat tatctactga cttccttcta tgtaagatga ggattgagct ctcttaccct
      961 tctcccagcg gccgcataac ttcgtatagc atacattata cgaagttatc tgacctcttc
     1021 tcttcctccc acagggcctc gagagatctg gcagcggaga gggcagagga agtcttctaa
     1081 catgcggtga cgtggaggag aatcccggcc ctaggattga acaagatgga ttgcacgcag
     1141 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     1201 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     1261 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     1321 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     1381 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     1441 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     1501 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     1561 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     1621 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     1681 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     1741 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     1801 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     1861 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctaacac ctggtgcatg
     1921 acccgcaagc ccggtgcctg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     1981 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     2041 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2101 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     2161 ctatggataa cttcgtatag catacattat acgaagttat gagctcacgg ggacagcccc
     2221 cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc
     2281 gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg
     2341 gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc
     2401 tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga
     2461 tttagaatga cagaatcata gaacggcctg ggttgcaaag gagcacagtg ctcatccaga
     2521 tccaaccccc tgctatgtgc agggtcatca accagcagcc caggctgccc agagccacat
     2581 ccagcctggc cttgaatgcc tgcagggatg gggcatccac agcctccttg ggcaacctgt
     2641 tcagtgcgtc accaccctct gggggaaaaa ctgcctcctc atatccaacc caaacctccc
     2701 ctgtctcagt gtaaagccat tcccccttgt cctatcaagg gggagtttgc tgtgacattg
     2761 ttggtctggg gtgacacatg tttgccaatt cagtgcatca cggagaggca gatcttgggg
     2821 ataaggaagt gcaggacagc atggacgtgg gacatgcagg tgttgagggc tctgggacac
     2881 tctccaagtc acagcgttca gaacagcctt aaggataaga agataggata gaaggacaaa
     2941 gagcaagtta aaacccagca tggagaggag cacaaaaagg ccacagacac tgctggtccc
     3001 tgtgtctgag cctgcatgtt tgatggtgtc tggatgcaag cagaaggggt ggaagagctt
     3061 gcctggagag atacagctgg gtcagtagga ctgggacagg cagctggaga attgccatgt
     3121 agatgttcat acaatcgtca aatcatgaag gctggaaaag ccctccaaga tccccaagac
     3181 caaccccaac ccacccaccg tgcccactgg ccatgtccct cagtgccaca tccccacagt
     3241 tcttcatcac ctccagggac ggtgaccccc ccacctccgt gggcagctgt gccactgcag
     3301 caccgctctt tggagaaggt aaatcttgct aaatccagcc cgaccctccc ctggcacaac
     3361 gtaaggccat tatctctcat ccaactccag gacggagtca gtgagaatat tggcgcgccg
     3421 aattctcgcg acgatcggtc gacataactt cgtataggat actttatacg aagttatcaa
     3481 ttcaggctag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     3541 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     3601 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     3661 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     3721 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     3781 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     3841 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     3901 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     3961 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
     4021 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
     4081 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
     4141 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
     4201 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
     4261 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
     4321 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     4381 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     4441 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
     4501 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     4561 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
     4621 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     4681 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     4741 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     4801 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     4861 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     4921 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     4981 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     5041 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     5101 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     5161 tgctggttat tgtgctgtct catcattttg gcaaagaatt gtgtacaacc atggagagcg
     5221 acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg
     5281 tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca
     5341 agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg
     5401 gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg
     5461 ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc
     5521 tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg
     5581 tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg
     5641 ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca
     5701 ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca
     5761 agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg
     5821 tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga
     5881 cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg
     5941 ccggacccgg ctccaccgga tctcgctaaa cgcgtgaatt cactcctcag gtgcaggctg
     6001 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     6061 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     6121 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     6181 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     6241 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     6301 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     6361 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     6421 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     6481 ctgtccctct tctcttatgg agatcaccgg tataacttcg tataatgtat actatacgaa
     6541 gttatggtac cttaattaag ttaacatgca tagtactgag ctcacgggga cagccccccc
     6601 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
     6661 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
     6721 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
     6781 ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt
     6841 agaatgacag aatcatagaa cggcctgggt tgcaaaggag cacagtgctc atccagatcc
     6901 aaccccctgc tatgtgcagg gtcatcaacc agcagcccag gctgcccaga gccacatcca
     6961 gcctggcctt gaatgcctgc agggatgggg catccacagc ctccttgggc aacctgttca
     7021 gtgcgtcacc accctctggg ggaaaaactg cctcctcata tccaacccaa acctcccctg
     7081 tctcagtgta aagccattcc cccttgtcct atcaaggggg agtttgctgt gacattgttg
     7141 gtctggggtg acacatgttt gccaattcag tgcatcacgg agaggcagat cttggggata
     7201 aggaagtgca ggacagcatg gacgtgggac atgcaggtgt tgagggctct gggacactct
     7261 ccaagtcaca gcgttcagaa cagccttaag gataagaaga taggatagaa ggacaaagag
     7321 caagttaaaa cccagcatgg agaggagcac aaaaaggcca cagacactgc tggtccctgt
     7381 gtctgagcct gcatgtttga tggtgtctgg atgcaagcag aaggggtgga agagcttgcc
     7441 tggagagata cagctgggtc agtaggactg ggacaggcag ctggagaatt gccatgtaga
     7501 tgttcataca atcgtcaaat catgaaggct ggaaaagccc tccaagatcc ccaagaccaa
     7561 ccccaaccca cccaccgtgc ccactggcca tgtccctcag tgccacatcc ccacagttct
     7621 tcatcacctc cagggacggt gaccccccca cctccgtggg cagctgtgcc actgcagcac
     7681 cgctctttgg agaaggtaaa tcttgctaaa tccagcccga ccctcccctg gcacaacgta
     7741 aggccattat ctctcatcca actccaggac ggagtcagtg agaatattgt ttaaactcct
     7801 catccttcca acataaatat attttgggat tatatcaaca ttcaatgtta cttaaagtga
     7861 ccttgtaaat attttcacaa ctgagccatg tttgatttgt atacttatgt ttactttact
     7921 gtttttcctg aagttaataa ttgccttgaa tttatttatt tctttaaaaa tgtttcatta
     7981 ctcaggactg tagtttacat tacgattctt tgtgttatac agttgatggg tttcttttct
     8041 ttcttaattt ctttaaaaaa tagagatggg gtcttactat attacccagg ctggtcttga
     8101 agtcctgggc tcaagtgatc ttcctgtctc agcctaccaa gtagctgaga ctataggtgc
     8161 aaaaaagcca ctatacctgg ctagtttaca ggttttaaca aatgcattat gccacgtatc
     8221 cattattaca ggatcacaca agatattttc attaccctga aagcatccct gtgttccacc
     8281 aattcatcct gcctccatga gccgctggca accactgatc tctatagttt tgccttttct
     8341 aaaatgtcat ataattggaa tcatacagtc tgtagcattt tcagactagc ttttaaaatt
     8401 tggcaatatg catttaaggt tcctccttaa atgtgaaggg catagccaat gtggcttgat
     8461 agctcatttc tttttattgg tgaatatttc attgtctgga tgttccacag tttgtttatc
     8521 cattcaccta ttcaatttgc tttttttctg tgtatctatc actaattcaa tactggactc
     8581 tccaacagag ccgtttaaac actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8641 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8701 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     8761 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     8821 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     8881 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     8941 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgatgcag
     9001 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     9061 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     9121 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     9181 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     9241 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     9301 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     9361 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     9421 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     9481 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     9541 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     9601 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     9661 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     9721 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     9781 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     9841 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     9901 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacattattc agattgggct
     9961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10201 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10741 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10801 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10861 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    10921 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa t
//
