LOCUS       sequence-27293-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: GST-GW1D12(aa895-1211)
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        77..434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        77..268
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(100..120)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      285..420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(454..471)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(454..470)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(463..485)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgncggang ggaattattc ggcgcagcac catggcctga aataacctct gaaagaggaa
       61 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
      121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      181 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      241 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
      301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
      361 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      421 taggcttttg caaaaagctg ttaacagctt ggcactggcc gtcgttttac aacgtcgtga
      481 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      541 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      601 tggcgaatgg cgcctgatgc ggtattttct cctnacgcat ctgtgcggta tttcacaccg
      661 catacntcnt aanagcaacc atagtacgcg ccctgtagcg gcncattaag cgcggcgggt
      721 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgcttgtttc
      781 gctttcttcc ttc
//
