LOCUS       sequence-272938-       10767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Components for genome editing in mammalian cells with pPB-CAG-hCas3 
            and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H,
            Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda 
            J, Mashimo T
  TITLE     CRISPR-Cas3 induces broad and unidirectional genome editing in human
            cells.
  JOURNAL   Nat Commun. 2019 Dec 6;10(1):5302. doi: 10.1038/s41467-019-13226-x.
  PUBMED    31811138
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-6"; volume: "10(1):5302. doi"; pages: " 
            10.1038/s41467-019-13226-x"
COMMENT     Sequence Label: pCAG-All-in-one-hCascade
FEATURES             Location/Qualifiers
     source          1..10767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        25..404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        406..683
                     /label=chicken beta-actin promoter
     intron          684..1700
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1624..1646
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1708..1727
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1754..1778
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1779..1788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1824..1844
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2967..2987
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3000..3056
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3096..3116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3630..3650
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3663..3719
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3759..3779
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5319..5339
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5352..5408
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5448..5468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6174..6194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6207..6263
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6303..6323
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6954..6974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6981..7005)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7034..7155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7045..7064)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7099..7118
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        7253..7632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7633..7836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7787..7807
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             7869..8468
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7869..7888)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8365..8385
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8506..8523)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8512..8736
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8761..8778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8932..9520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9012..9031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9691..10482,10483..10551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10314..10333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10552..10656)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10724..10742
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caactttgta tagaaaagtt gctcgacatt gattattgac tagttattaa tagtaatcaa
       61 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      121 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      181 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      241 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      301 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      361 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      421 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      481 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggnnncgc gcgccaggcg
      541 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      601 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      661 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      721 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      781 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      841 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
      901 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
      961 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     1021 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     1081 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     1141 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     1201 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     1261 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     1321 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     1381 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     1441 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     1501 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     1561 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     1621 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     1681 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     1741 ttggcaaaga attgcaagtt tgtacaaaaa agcaggctgc caccatggcc aagagaacag
     1801 ccgatggcag cgagttcgag agccccaaga agaagagaaa ggtcgagtcc aacttcatca
     1861 acatccacgt gctgatctct cacagcccca gctgcctgaa ccgggacgac atgaacatgc
     1921 agaaggacgc catcttcggc ggcaagagaa gagtgcggat cagcagccag agcctgaagc
     1981 gggccatgag aaagagcggc tactacgccc agaacatcgg cgagagcagc ctgagaacaa
     2041 tccatctggc ccagctgcgg gacgtgctga gacagaaact gggcgagaga ttcgaccaga
     2101 agatcatcga caagacactg gccctgctga gcggcaagtc tgtggatgag gccgagaaga
     2161 tcagcgccga tgctgtgaca ccttgggtcg tgggagagat cgcctggttc tgtgaacagg
     2221 tggccaaggc cgaggccgac aacctggacg ataagaaact gctgaaggtg ctgaaagagg
     2281 atatcgccgc catcagagtg aacctgcagc agggcgttga cattgccctg tctggcagaa
     2341 tggccaccag cggcatgatg accgagctgg gaaaagtgga cggcgccatg tctatcgccc
     2401 acgccattac aacacaccag gtggacagcg acatcgactg gttcaccgcc gtggacgatc
     2461 tgcaagaaca gggatctgcc cacctgggca cccaagagtt tagcagcggc gtgttctaca
     2521 gatacgccaa catcaacctg gctcagctgc aagagaatct cggcggagcc tctagagagc
     2581 aggccctgga aattgccaca cacgtggtgc acatgctggc cacagaagtg cctggcgcca
     2641 agcagagaac ctacgccgcc tttaatcccg ccgacatggt catggtcaac ttcagcgaca
     2701 tgcccctgag catggccaac gcctttgaga aggccgtgaa ggccaaggac ggcttcctgc
     2761 agcctagcat ccaggccttc aatcagtact gggacagagt ggccaacggc tacggactga
     2821 atggtgccgc cgctcagttt agcctgtccg acgtggaccc tatcacagcc caagtgaagc
     2881 agatgcccac actggaacag ctgaagtcct gggtccgaaa caacggcgag gccaaaagaa
     2941 ccgctgacgg ctccgagttt gagtccccta agaagaaacg gaaggttgaa ggcagcggcg
     3001 ccaccaattt cagcctgctg aaacaggctg gcgacgtgga agagaaccct ggacctatgg
     3061 ccaaacggac cgccgacgga agcgaattcg agtctcccaa gaaaaagcgc aaggtggaag
     3121 ccgacgagat cgacgccatg gcactgtata gagcctggca gcagctggac aatggcagct
     3181 gtgcccagat cagaagagtg tccgagcctg acgagctgag agacatcccc gccttctaca
     3241 gactggtgca gcctttcggc tgggagaacc ctagacatca gcaggcactg ctgcggatgg
     3301 tgttctgtct gagcgccggc aagaacgtga tccggcacca ggataagaag tccgagcaga
     3361 ccacaggcat cagcctgggt agagccctgg ccaatagcgg cagaatcaac gagcggcgga
     3421 tcttccagct gatcagagcc gatagaaccg ccgatatggt gcagctcaga cggctgctga
     3481 cacacgctga acctgtgctg gattggcccc tgatggccag aatgctgact tggtggggaa
     3541 agagagagcg ccagcagctc ctggaagatt tcgtgctgac caccaacaag aacgccaaga
     3601 ggactgccga cggctcagaa ttcgaaagcc caaagaaaaa acgaaaagtc gaaggctctg
     3661 gggccacgaa ctttagtctg ctgaagcaag ccggggacgt cgaagaaaat ccaggaccaa
     3721 tggctaagcg caccgctgat ggatctgaat ttgaatctcc caaaaagaag cggaaagtcg
     3781 agaacctgct gatcgacaac tggatccccg tgcggcctag aaatggcggc aaggtgcaga
     3841 tcatcaatct gcagagcctg tactgcagcc gggatcagtg gcgactgagc ctgcctagag
     3901 atgacatgga actggccgct ctggctctgc tcgtgtgcat cggacagatt atcgcccctg
     3961 ccaaggatga tgtggaattc cggcacagaa tcatgaaccc tctgaccgag gacgagttcc
     4021 agcagctgat cgccccttgg atcgacatgt tctacctgaa ccacgccgag catcccttca
     4081 tgcagaccaa gggcgtgaaa gccaacgatg tgacccctat ggaaaaactg ctggccggcg
     4141 tttccggcgc cacaaactgt gcctttgtga accagcctgg acagggcgaa gctctgtgtg
     4201 gcggatgtac agcaatcgcc ctgttcaacc aggccaatca ggcccctgga ttcggcggag
     4261 gctttaagtc tggactgaga ggcggaaccc ctgtgaccac atttgtgcgg ggcatcgacc
     4321 tgagaagcac cgtgctgctg aacgtcctga cactgcccag actgcagaag cagttcccca
     4381 acgagagcca caccgagaac cagccaacct ggatcaagcc catcaagagc aacgagtcta
     4441 tccccgccag ctccatcggc tttgtcagag gcctgttttg gcagcccgct cacatcgagc
     4501 tgtgcgaccc tatcggcatc ggcaagtgta gctgttgcgg ccaagagtcc aacctgcggt
     4561 acaccggctt tctgaaagag aagttcacct tcaccgtgaa cggcttgtgg ccccatccac
     4621 acagcccttg tctcgtgacc gtgaagaaag gcgaggtcga ggaaaagttc ctggccttta
     4681 ccacaagcgc ccctagctgg acccagatct ctagagtggt ggtggacaag atcatccaga
     4741 acgagaacgg caatagagtg gccgccgtgg tcaaccagtt ccggaatatt gcccctcaga
     4801 gccctctgga actgatcatg ggcggctacc ggaacaacca ggctagcatc ctggaaagac
     4861 ggcacgatgt gctgatgttc aatcaaggat ggcagcagta cggcaacgtg atcaacgaga
     4921 tcgtgacagt cggcctgggc tacaagacag ccctgagaaa ggccctgtac accttcgccg
     4981 agggcttcaa gaacaaggac ttcaaaggcg caggcgtgtc cgtgcacgaa acagccgaga
     5041 gacacttcta ccggcagagc gagctgctga ttcctgacgt gctggccaac gtgaacttca
     5101 gccaggccga tgaagtgatc gccgacctga gggacaaact gcatcagctg tgtgaaatgc
     5161 tgtttaacca gagcgtggcc ccttacgctc atcaccccaa gctgattagc actctggccc
     5221 tggctagagc taccctgtac aagcacctga gagagctgaa gcctcaaggc ggccctagca
     5281 atggaaagcg gacagcagat ggatccgagt tcgaaagtcc gaaaaaaaag aggaaagttg
     5341 aaggatccgg cgctactaac tttagcctcc tcaagcaggc tggggatgtt gaagaaaacc
     5401 ccggtcctat ggctaagaga accgcagatg gctccgaatt tgaaagccct aaaaagaaaa
     5461 gaaaggtgga acgcagctac ctgatcctga gactggccgg acctatgcag gcttggggac
     5521 agcctacctt cgagggcaca agacccaccg gcagattccc tacaagaagc ggcctgctgg
     5581 gactgcttgg agcctgtctg ggaatccaga gggacgatac aagctccctg caggctctga
     5641 gcgagagcgt gcagtttgcc gtcagatgcg acgaactgat cctggatgac agacgggtgt
     5701 ccgtgaccgg cctgagagat taccatacag tgctgggcgc cagagaggat taccggggac
     5761 tgaaaagcca cgaaaccatc cagacctggc gcgagtacct gtgcgacgcc tcttttacag
     5821 tggccctgtg gctgacacct cacgccacaa tggtcatctc tgagctggaa aaggccgtgc
     5881 tgaagccccg gtacacacct tacctgggca gaagaagctg ccctctgaca caccctctgt
     5941 ttctgggcac ctgtcaggcc agcgatccac agaaagctct gctgaactac gagcctgtcg
     6001 gcggcgacat ctacagcgag gaatctgtga caggccacca cctgaagttt accgccagag
     6061 atgagcctat gatcaccctg cctcggcagt tcgcctccag agagtggtac gttatcaaag
     6121 gcggcatgga tgtgtcccag aagcgcacag cagacggcag tgaatttgag agtcctaaga
     6181 aaaagaggaa ggtcgaaggt tcaggggcaa caaacttctc acttctgaag caggccggcg
     6241 acgttgaaga gaacccaggt ccaatggcaa aaaggacagc tgacggtagc gagttcgaat
     6301 caccaaaaaa gaaacgcaag gtcgagtacc tcagcaaagt gatcattgcc agagcttgga
     6361 gcagggacct gtaccagctg catcaaggcc tgtggcatct gttccccaat agacccgacg
     6421 ccgccaggga tttcctgttt cacgtggaaa agcggaacac ccctgagggc tgtcatgtgc
     6481 tgctgcagag tgctcagatg ccagtgtcaa ctgccgtggc caccgtgatc aagaccaagc
     6541 aggtcgaatt ccagctccaa gtgggcgtgc ccctgtactt cagactgaga gccaatccta
     6601 tcaagacgat cctggacaac cagaagcggc tggacagcaa gggcaacatc aagagatgca
     6661 gggtgcccct gatcaaagag gccgaacaga ttgcctggct gcagcggaaa ctgggaaatg
     6721 ccgccagagt ggaagatgtg caccccatca gcgagcggcc tcagtacttt agcggagatg
     6781 gcaagtccgg caagatccag accgtgtgct ttgagggcgt gctgacaatc aacgacgccc
     6841 ctgctctgat cgacctggtc cagcaaggca ttggcccagc caagtctatg ggctgtggac
     6901 tgctgtctct ggcccctctg aaacgcactg cagatggaag cgagtttgaa tccccaaaga
     6961 aaaaaagaaa ggttgagtga acccagcttt cttgtacaaa gtggtgatgg ccggccgctt
     7021 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     7081 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     7141 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     7201 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtacgcg ttgacattga
     7261 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     7321 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     7381 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     7441 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     7501 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     7561 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     7621 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     7681 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     7741 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     7801 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     7861 gcgccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7921 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7981 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     8041 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     8101 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     8161 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     8221 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     8281 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     8341 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     8401 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     8461 gtgcctgact cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     8521 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8581 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8641 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8701 atagcaggca tgctggggat gcggtgggct ctatgggcgg ccgcggcgct cttccgcttc
     8761 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8821 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8881 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8941 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9001 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9061 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc tcttcgggaa gcgtggcgct
     9121 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9181 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9241 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9301 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9361 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9421 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9601 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9661 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9721 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9781 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9841 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9901 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9961 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10021 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10081 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10141 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10201 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10261 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10321 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10381 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10441 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10501 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10561 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10621 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10681 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10741 gccctttcgt cggcgcgccg cggccgc
//
