LOCUS       sequence-272942-        8631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A-to-G base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, 
            Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR
  TITLE     Phage-assisted evolution of an adenine base editor with improved Cas
            domain compatibility and activity.
  JOURNAL   Nat Biotechnol. 2020 Jul;38(7):883-891. doi: 
            10.1038/s41587-020-0453-z. Epub 2020 Mar 16.
  PUBMED    32433547
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0453-z"; journalName: "Nat Biotechnol"; date: 
            "2020-07"; volume: "38"; issue: "7"; pages: "883-891"
COMMENT     Sequence Label: enAsABE8e-dimer
FEATURES             Location/Qualifiers
     source          1..8631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1654..5574
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5294..5303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5617..5637
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5646..5663
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5686..5703)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5692..5916
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5987..6003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5987..6003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6000..6022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6011..6027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6035..6041,6042..6059,6060..6065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6080..6101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6218..6235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6389..6977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6469..6488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7148..7939,7940..8008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7771..7790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8009..8113)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8192..8211)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8383..8631,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga agtcgagttt
      481 agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga
      541 gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac
      601 aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga
      661 ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca
      721 tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca
      781 cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg
      841 aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc
      901 gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc
      961 gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc
     1021 gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac
     1081 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgggatga gagggaggtg
     1141 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc
     1201 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg
     1261 gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg
     1321 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac
     1381 tcaaaaagag gcgccgcagg ctccctgatg aacgtgctga actaccccgg catgaatcac
     1441 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgcgatttc
     1501 tatcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc catcaactcc
     1561 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca
     1621 cctgaaagca gcgggggcag cagcgggggg tcaatgacac agttcgaggg ctttaccaac
     1681 ctgtatcagg tgagcaagac actgcggttt gagctgatcc cacagggcaa gaccctgaag
     1741 cacatccagg agcagggctt catcgaggag gacaaggccc gcaatgatca ctacaaggag
     1801 ctgaagccca tcatcgatcg gatctacaag acctatgccg accagtgcct gcagctggtg
     1861 cagctggatt gggagaacct gagcgccgcc atcgactcct atagaaagga gaaaaccgag
     1921 gagacaagga acgccctgat cgaggagcag gccacatatc gcaatgccat ccacgactac
     1981 ttcatcggcc ggacagacaa cctgaccgat gccatcaata agagacacgc cgagatctac
     2041 aagggcctgt tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg
     2101 accacaaccg agcacgagaa cgccctgctg cggagcttcg acaagtttac aacctacttc
     2161 tccggctttt atagaaacag gaagaacgtg ttcagcgccg aggatatcag cacagccatc
     2221 ccacaccgca tcgtgcagga caacttcccc aagtttaagg agaattgtca catcttcaca
     2281 cgcctgatca ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa gaaggccatc
     2341 ggcatcttcg tgagcacctc catcgaggag gtgttttcct tcccttttta taaccagctg
     2401 ctgacacaga cccagatcga cctgtataac cagctgctgg gaggaatctc tcgggaggca
     2461 ggcaccgaga agatcaaggg cctgaacgag gtgctgaatc tggccatcca gaagaatgat
     2521 gagacagccc acatcatcgc ctccctgcca cacagattca tccccctgtt taagcagatc
     2581 ctgtccgata ggaacaccct gtctttcatc ctggaggagt ttaagagcga cgaggaagtg
     2641 atccagtcct tctgcaagta caagacactg ctgagaaacg agaacgtgct ggagacagcc
     2701 gaggccctgt ttaacgagct gaacagcatc gacctgacac acatcttcat cagccacaag
     2761 aagctggaga caatcagcag cgccctgtgc gaccactggg atacactgag gaatgccctg
     2821 tatgagcgga gaatctccga gctgacaggc aagatcacca agtctgccaa ggagaaggtg
     2881 cagcgcagcc tgaagcacga ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag
     2941 gagctgagcg aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc
     3001 ctggatcagc cactgcctac aaccctgaag aagcaggagg agaaggagat cctgaagtct
     3061 cagctggaca gcctgctggg cctgtaccac ctgctggact ggtttgccgt ggatgagtcc
     3121 aacgaggtgg accccgagtt ctctgcccgg ctgaccggca tcaagctgga gatggagcct
     3181 tctctgagct tctacaacaa ggccagaaat tatgccacca agaagcccta ctccgtggag
     3241 aagttcaagc tgaactttca gatgcctaca ctggccagag gctgggacgt gaatagagag
     3301 aagaacaatg gcgccatcct gtttgtgaag aacggcctgt actatctggg catcatgcca
     3361 aagcagaagg gcaggtataa ggccctgagc ttcgagccca cagagaaaac cagcgagggc
     3421 tttgataaga tgtactatga ctacttccct gatgccgcca agatgatccc aaagtgcagc
     3481 acccagctga aggccgtgac agcccacttt cagacccaca caacccccat cctgctgtcc
     3541 aacaatttca tcgagcctct ggagatcaca aaggagatct acgacctgaa caatcctgag
     3601 aaggagccaa agaagtttca gacagcctac gccaagaaaa ccggcgacca gaagggctac
     3661 agagaggccc tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa gtataccaag
     3721 acaacctcta tcgatctgtc tagcctgcgg ccatcctctc agtataagga cctgggcgag
     3781 tactatgccg agctgaatcc cctgctgtac cacatcagct tccagagaat cgccgagaag
     3841 gagatcatgg atgccgtgga gacaggcaag ctgtacctgt tccagatcta taacaaggac
     3901 tttgccaagg gccaccacgg caagcctaat ctgcacacac tgtattggac cggcctgttt
     3961 tctccagaga acctggccaa gacaagcatc aagctgaatg gccaggccga gctgttctac
     4021 cgccctaagt ccaggatgaa gaggatggca caccggctgg gagagaagat gctgaacaag
     4081 aagctgaagg atcagaaaac cccaatcccc gacaccctgt accaggagct gtacgactat
     4141 gtgaatcaca gactgtccca cgacctgtct gatgaggcca gggccctgct gcccaacgtg
     4201 atcaccaagg aggtgtctca cgagatcatc aaggataggc gctttaccag cgacaagttc
     4261 tttttccacg tgcctatcac actgaactat caggccgcca attccccatc taagttcaac
     4321 cagagggtga atgcctacct gaaggagcac cccgagacac ctatcatcgg catcgcccgg
     4381 ggtgagagaa acctgatcta tatcacagtg atcgactcca ccggcaagat cctggagcag
     4441 cggagcctga acaccatcca gcagtttgat taccagaaga agctggacaa cagggagaag
     4501 gagagggtgg cagcaaggca ggcctggtct gtggtgggca caatcaagga tctgaagcag
     4561 ggctatctga gccaggtcat ccacgagatc gtggacctga tgatccacta ccaggccgtg
     4621 gtggtgctgg agaacctgaa tttcggcttt aagagcaaga ggaccggcat cgccgagaag
     4681 gccgtgtacc agcagttcga gaagatgctg atcgataagc tgaattgcct ggtgctgaag
     4741 gactatccag cagagaaagt gggaggcgtg ctgaacccat accagctgac agaccagttc
     4801 acctcctttg ccaagatggg cacccagtct ggcttcctgt tttacgtgcc tgccccatat
     4861 acatctaaga tcgatcccct gaccggcttc gtggacccct tcgtgtggaa aaccatcaag
     4921 aatcacgaga gccgcaagca cttcctggag ggcttcgact ttctgcacta cgacgtgaaa
     4981 accggcgact tcatcctgca ctttaagatg aacagaaatc tgtccttcca gaggggcctg
     5041 cccggcttta tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc
     5101 aagggcaccc ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa tcacagattc
     5161 accggcagat accgggacct gtatcctgcc aacgagctga tcgccctgct ggaggagaag
     5221 ggcatcgtgt tcagggatgg ctccaacatc ctgccaaagc tgctggagaa tgacgattct
     5281 cacgccatcg acaccatggt ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat
     5341 gccgccacag gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc
     5401 gactcccggt ttcagaaccc agagtggccc atggacgccg atgccaatgg cgcctaccac
     5461 atcgccctga agggccagct gctgctgaat cacctgaagg agagcaagga tctgaagctg
     5521 cagaacggca tctccaatca ggactggctg gcctacatcc aggagctgcg caactctggc
     5581 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtctaa
     5641 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     5701 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5761 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5821 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5881 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5941 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6001 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6061 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6421 acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac actcagtgga
     7021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8161 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     8221 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     8281 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     8341 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     8401 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     8461 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     8521 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     8581 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat c
//
