LOCUS       sequence-27296-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: FLAG-PAZ(aa1-480)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..117
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    130..154
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggggagtc aatagcagag ctcgtttagt gaaccgtcag aattaaccat ggactacaaa
       61 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagctt
      121 gcggccatca caagtttgta caaaaaagca ggctctttaa aggaaccaat tcagtcgact
      181 ggatccatgc acccattcca gtggtgtaac gcacttgcac ctcctgcgcc gccgcccccc
      241 atccaaggat atgccttcaa gcctccacct agacccgact ttgggacctc cgggagaaca
      301 atcaaattac aggccaattt cttcgaaatg gacatcccca aaattgacat ctatcattat
      361 gaattggata tcaagccaga gaagtgcccg aggagagtta acagggaaat cgtggaacac
      421 atggtccagc actttaaaac acagatcttt ggggatcgga agcccgtgtt tgacggcagg
      481 aagaatctat acacagccat gccccttccg attgggaggg acaaggtgga gctggaggtc
      541 acgctgccag gagaaggcaa ggatcgcatc ttcaaggtgt ccatcaagtg ggtgtcctgc
      601 gtgagcttgc aggcgttaca cgatgcactt tcagggcggc tgcccagcgt cccttttgag
      661 acgatccagg ccctggacgt gggtcatgag gcacttgcca tccatgaggt agcacccccg
      721 cgtcggtgcc gctccctcct tcttccaccg cgtacggaag gctgctctaa ccctcttggc
      781 gggggcccga agaaggtgtg
//
