LOCUS       sequence-272998-       10868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retrovirus construct to express mCherry-Halo-YAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Maruyama J, Inami K, Michishita F, Jiang X, Iwasa H, Nakagawa K, 
            Ishigami-Yuasa M, Kagechika H, Miyamura N, Hirayama J, Nishina H, 
            Nogawa D, Yamamoto K, Hata Y
  TITLE     Novel YAP1 Activator, Identified by Transcription-Based Functional 
            Screen, Limits Multiple Myeloma Growth.
  JOURNAL   Mol Cancer Res. 2018 Feb;16(2):197-211. doi: 
            10.1158/1541-7786.MCR-17-0382. Epub 2017 Oct 23.
  PUBMED    29061667
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1541-7786.MCR-17-0382"; journalName: "Mol Cancer Res"; 
            date: "2018-02"; volume: "16"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: pQCXIP-mCherry-Halo-YAP1
FEATURES             Location/Qualifiers
     source          1..10868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             585..760
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    823..1022
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1223..1639
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1569..1591
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1607..1623
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1680..2842
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1911..2833
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2790..2810
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2863..2872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2869..3576
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3014..3032)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3282..3301
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3583..4470
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             4483..4503
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    6178..6640
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6234..6251)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6461..6480
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6673..7272
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6673..6692)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7169..7189
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7646..8071
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(8174..8196)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8332..8352)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8337..8666
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8517..8652
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8688..8707
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8788..8805)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8959..9547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9039..9058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9718..10509,10510..10578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10341..10360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10579..10683)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10751..10769
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcaataa aagagcccac
      601 aacccctcac tcggcgcgcc agtcttccga tagactgcgt cgcccgggta cccgtattcc
      661 caataaagcc tcttgctgtt tgcatccgaa tcgtggtctc gctgttcctt gggagggtct
      721 cctctgagtg attgactacc cacgacgggg gtctttcatt tgggggctcg tccgggattt
      781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
      841 tctgtgtctg tccgattgtc tagtgtctat gtttgatgtt atgcgcctgc gtctgtacta
      901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc
      961 cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg
     1021 aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga
     1081 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg
     1141 cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1201 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1261 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1321 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1381 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1441 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1501 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1561 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1621 cactccttct ctaggcgccg gaattgaaga tctgggggat cgatcctcta gcttcgtgag
     1681 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     1741 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     1801 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     1861 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     1921 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     1981 cttccacctg gctccagtac gtgattcttg atcccgagct ggagccaggg gcgggccttg
     2041 cgctttagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2101 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2161 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2221 tgcgggccag gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2281 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2341 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2401 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2461 atggccgctt cccggccctg ctccaggggg ctcaaaatgg aggacgcggc gctcgggaga
     2521 gcgggcgggt gagtcaccca cacaaaggaa aggggccttt ccgtcctcag ccgtcgcttc
     2581 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct ggagcttttg
     2641 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2701 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     2761 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     2821 tcttccattt caggtgtcgt gaacacgtgg tcgcggcctc tagccaccat ggtgagcaag
     2881 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     2941 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     3001 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     3061 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     3121 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3181 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     3241 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     3301 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     3361 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     3421 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     3481 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     3541 cgccactcca ccggcggcat ggacgagctg tacaaggaat tggcagaaat cggtactggc
     3601 tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt
     3661 ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg
     3721 tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc
     3781 ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc
     3841 atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg
     3901 ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca
     3961 tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag
     4021 accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt
     4081 tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac
     4141 cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac
     4201 gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac
     4261 tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc
     4321 ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc
     4381 ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg
     4441 cgctggctgt cgacgctcga gatttccggc gagccaacca ctgaggatct gtactttcag
     4501 agcgataacg cggaattcac gcgtcccggg cagcagccgc cgcctcaacc ggccccccag
     4561 ggccaagggc agccgccttc gcagcccccg caggggcagg gcccgccgtc cggacccggg
     4621 caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac cccccgccgg gcatcagatc
     4681 gtgcacgtcc gcggggactc ggagaccgac ctggaggcgc tcttcaacgc cgtcatgaac
     4741 cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc tccggaagct gcccgactcc
     4801 ttcttcaagc cgccggagcc caaatcccac tcccgacagg ccagtactga tgcaggcact
     4861 gcaggagccc tgactccaca gcatgttcga gctcattcct ctccagcttc tctgcagttg
     4921 ggagctgttt ctcctgggac actgaccccc actggagtag tctctggccc agcagctaca
     4981 cccacagctc agcatcttcg acagtcttct tttgagatac ctgatgatgt acctctgcca
     5041 gcaggttggg agatggcaaa gacatcttct ggtcagagat acttcttaaa tcacatcgat
     5101 cagacaacaa catggcagga ccccaggaag gccatgctgt cccagatgaa cgtcacagcc
     5161 cccaccagtc caccagtgca gcagaatatg atgaactcgg cttcaggtcc tcttcctgat
     5221 ggatgggaac aagccatgac tcaggatgga gaaatttact atataaacca taagaacaag
     5281 accacctctt ggctagaccc aaggcttgac cctcgttttg ccatgaacca gagaatcagt
     5341 cagagtgctc cagtgaaaca gccaccaccc ctggctcccc agagcccaca gggaggcgtc
     5401 atgggtggca gcaactccaa ccagcagcaa cagatgcgac tgcagcaact gcagatggag
     5461 aaggagaggc tgcggctgaa acagcaagaa ctgcttcggc aggcaatgcg gaatatcaat
     5521 cccagcacag caaattctcc aaaatgtcag gagttagccc tgcgtagcca gttaccaaca
     5581 ctggagcagg atggtgggac tcaaaatcca gtgtcttctc ccgggatgtc tcaggaattg
     5641 agaacaatga cgaccaatag ctcagatcct ttccttaaca gtggcaccta tcactctcga
     5701 gatgagagta cagacagtgg actaagcatg agcagctaca gtgtccctcg aaccccagat
     5761 gacttcctga acagtgtgga tgagatggat acaggtgata ctatcaacca aagcaccctg
     5821 ccctcacagc agaaccgttt cccagactac cttgaagcca ttcctgggac aaatgtggac
     5881 cttggaacac tggaaggaga tggaatgaac atagaaggag aggagctgat gccaagtctg
     5941 caggaagctt tgagttctga catccttaat gacatggagt ctgttttggc tgccaccaag
     6001 ctagataaag aaagctttct tacatggtta taggtcgacc cgggcggccg ctcgagttta
     6061 aacaattgga tccaattccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     6121 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     6181 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     6241 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     6301 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     6361 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6421 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6481 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6541 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     6601 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccacaagg
     6661 agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6721 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6781 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6841 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6901 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6961 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     7021 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     7081 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     7141 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     7201 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     7261 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     7321 catggctccg accgaagcca cccggggcgg ccccgccgac cccgcacccg cccccgaggc
     7381 ccaccgactc tagtcgaggg ctgcagcgct gcagaggccg agtgcagaac tgctccaaag
     7441 ggacctcaag gctttccgag ggacactagg ctgactccat cgagccagtg tagagataag
     7501 cttatcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     7561 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     7621 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     7681 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca
     7741 gatcaaggtc aggaacagat ggaacagggt cgaccctaga gaaccatcag atgtttccag
     7801 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     7861 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     7921 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     7981 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     8041 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc
     8101 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg
     8161 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     8221 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     8281 gcgcagccat gacccagtca cgtagcgata gcggagtgta gatccggctg tggaatgtgt
     8341 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8401 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     8461 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8521 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8581 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8641 tttttggagg cctaggcttt tgcaaaaagc ttactggctt aactatgcgg catcagagca
     8701 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     8761 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9361 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9961 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10021 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10141 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10261 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10321 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10381 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10441 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10681 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10741 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattag cttggccatt
    10801 gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc
    10861 gccatgtt
//
