LOCUS       sequence-273014-        9905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enables high-efficiency recombineering in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Wannier TM, Nyerges A, Kuchwara HM, Czikkely M, Balogh D, Filsinger 
            GT, Borders NC, Gregg CJ, Lajoie MJ, Rios X, Pal C, Church GM
  TITLE     Improved bacterial recombineering by parallelized protein discovery.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13689-13698. doi: 
            10.1073/pnas.2001588117. Epub 2020 May 28.
  PUBMED    32467157
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2001588117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-06-16- 16"; volume: "117"; issue: "24"; pages: 
            "13689-13698"
COMMENT     Sequence Label: pORTMAGE-Ec1
FEATURES             Location/Qualifiers
     source          1..9905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            55..163
                     /note="incP origin of transfer"
     CDS             complement(174..1025)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1012..1851)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(2362..3333)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      3440..3834
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(3872..3958)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3983..4948)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        5848..5928
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      8752..8846
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(8967..8986)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8978..9793
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
       61 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
      121 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gcctcagcct
      181 gccgccttgg gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc
      241 agcgcgacca gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa
      301 ccactggcct ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt
      361 ttgccggggt cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc
      421 agcgcccgca cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc
      481 gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc
      541 gacagcagcc gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa
      601 aggcgctcga tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg
      661 atttcctttg ccagcgcccg atagctacct ttgaccacca tggcatcagc ggtgacggcc
      721 tcccacttgg gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg
      781 ccaagcacta ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca
      841 tcagcgccca gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac
      901 gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg
      961 gccagacagt gcgccgggtc gtgccggacg tggctgaggc tgtgcttgtt cttaggcttc
     1021 accacggggc acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc
     1081 gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac
     1141 accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc
     1201 gctggtcatg ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc
     1261 ccggctggcc tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg
     1321 caggaacacg atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg
     1381 ggccatgggg ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac
     1441 catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat
     1501 gttgggcagg ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc
     1561 ctcggcgctg aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc
     1621 ttcggcgggc aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg
     1681 cccgcctgca atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg
     1741 cgacaccagc gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg
     1801 cggcgctgct gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct
     1861 cctttgcagg cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct
     1921 ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg
     1981 cgctgacgca tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca
     2041 gggctggcca gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag
     2101 aaacttgccg gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag
     2161 gttaaggctg gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata
     2221 accaaagcca ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga
     2281 agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag
     2341 gcgtgagtac caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg
     2401 ctggcggggt gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc
     2461 catgaccttg ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc
     2521 cagcgctggg ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct
     2581 ggccagcttc tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac
     2641 cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg
     2701 ccgctcgggc agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg
     2761 ctcgatctgc tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt
     2821 ggattcacgc agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg
     2881 gttggtgaag cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc
     2941 gtactcgctg gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc
     3001 caccttgacc catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg
     3061 gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc
     3121 atgccgctcc tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt
     3181 gagccatggc gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc
     3241 ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg
     3301 gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca ccaagcgatt cccacacatt
     3361 atacgagccg gaagcataaa gtgtagctag atccgaagga tgagccgggc tgaatgatcg
     3421 accgagacag gccctgcggg gctgcacacg cgcccccacc cttcgggtag ggggaaaggc
     3481 cgctaaagcg gctaaaagcg ctccagcgta tttctgcggg gtttggtgtg gggtttagcg
     3541 ggctttgccc gcctttcccc ctgccgcgca gcggtggggc ggtgtgtagc ctagcgcagc
     3601 gaatagacca gctatccggc ctctggccgg gcatattggg caagggcagc agcgccccac
     3661 aagggcgctg ataaccgcgc ctagtggatt attcttagat aatcatggat ggatttttcc
     3721 aacaccccgc cagcccccgc ccctgctggg tttgcaggtt tgggggcgtg acagttattg
     3781 caggggttcg tgacagttat tgcagggggg cgtgacagtt attgcagggg ttcgtgacag
     3841 ttagggcgcg cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
     3901 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     3961 gcctttaatt aaacgttcgt aatcaagcca cttccttttt gcattgacgc agggtgtcgg
     4021 aaggcaactc gccgaacgcg ctcctatagt tttcagcgaa gcgtcccaaa tgtaagaagc
     4081 cgtagtctag ggctatctca gttatactac gcacattggc actgggatcg ttcaagcagg
     4141 cgcggatgct ttcgagcttg cggttgcgga tgtagttctt cggcgtggtg ccggcgtgct
     4201 tctcgaacaa attgtagagc gagcgtggac tcatcatcgc cagctccgct aaccgctcaa
     4261 ggctgatatt ccgtttgaga ttctcctcaa tgaattgaac gactcgctcg aaagacgggt
     4321 tacctttgct gaaaatttca cggctgacat tgctgcccag catttcgagc agcttggaag
     4381 cgatgatccc cgcatagtgc tcttggaccc gaggcatcga ctttgtatgt tccgcttcgt
     4441 cacaaactaa cccgagtaga ttgataaagc catcgagttg ctggagattg tgtcgcgcgg
     4501 cgaaacggat accctccctc ggcttgtgcc aattgttgtc actgcacgcc cgatcaagga
     4561 ccactgaggg caatttaacg ataaatttct cgcaatcttc tgaataggtc aggtcggctt
     4621 ggtcatccgg attgagcagc aatagttcgc ccggcgcaaa atagtgctcc tggccatggc
     4681 cacgccacag gcaatggcct ttgagtatta tttgcagatg ataacaggtc tctaatccag
     4741 gcgagattac cctcacgcta ccgccgtagc tgattcgaca cagatcgagg catccgaaga
     4801 ttctgtggtg cagcctgcct gccgggcgcc cgcccttggg caggcgaata gagtgcgtac
     4861 cgacatactg gttaacataa tcggagactg catagggctc ggcgtggacg aagatctgac
     4921 ttttctcgtt caataagcaa aaatccatag ttcacggttc tcttatttta atgtgggctg
     4981 cttggtgtga tgtagaaagg cgccaagtcg atgaaaatgc atctcgacgt gatgcgtata
     5041 cgggttaccc ccattgccac gttgcgccat cctttttgca atcagtgacc acttttccaa
     5101 gcaaaaataa cgccaagcag aacgaagacg ttctttttaa gaagcgagaa caccagaagt
     5161 tcgtgctgtc ggggcatggg gcgacgaatt ggcggataaa ggggatctgc tggatattac
     5221 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     5281 tcttgcccgc ctgatgaatg ctcatccgta attacgtatg gcaatgaaag acggtgagct
     5341 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     5401 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     5461 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     5521 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     5581 tatggacaac ttcttcgccc ccgttttcac catgcatggg aattagcttg atctgaccaa
     5641 cgaccggtag cggagctatc caacggcggt ataccaggaa aacacacagc aggtacatca
     5701 gaacagtacc atgactgaag aacaaatagt tttttcctga tccataaagc agaacggcct
     5761 gctccatgac aaatctggct ccccaactaa tgccccatgc agccagcata accagcataa
     5821 agtgcagtgt ccggtttgat agggataagt ccagccttgc aagaagcgga tacaggagtg
     5881 caaaaaatgg ctatctctag taaggcctac cccttaggct ttatgcaaca gaaacaataa
     5941 taatggagtc atgaccatgc ctaggccgcg ggccgcgcga attcagaagg agaatatacc
     6001 atgaaccaaa tcgtgaagtt cactgacgac tctggcctgg cggttcaagt tactccagac
     6061 gatgttcgcc gttatatctg tgagaacgct actgaaaaag aggtgggcct ctttctgcaa
     6121 ctctgtcaga ctcaacgtct gaatccgttt gtgaaagacg cttacctggt gaaatacggc
     6181 ggtgctccag cttctatgat tacttcctat caagttttta accgtcgcgc gtgtcgtgat
     6241 gctaactatg atggtatcaa atctggtgtg gttgttctgc gtgacggtga tgttgtgcat
     6301 aaacgtggtg ctgcgtgcta caaaaaggcg ggtgaggagc tcatcggtgg ttgggcggaa
     6361 gttcgcttta aggatggccg cgagactgcg tatgctgagg tggcgctcga cgactattcc
     6421 accggcaaat ctaattgggc gaaaatgccg ggtgttatga tcgaaaaatg cgcgaaggct
     6481 gctgcttggc gcctcgcgtt cccggacact tttcagggca tgtacgctgc ggaggaaatg
     6541 gatcaagcgc aacagccaga acaggtgcgc gctcaggcgg agcaaccagt ggatctccag
     6601 ccaatccgcg aactcttcaa gccatattgc gaacacttcg gcatcactcc ggctgagggt
     6661 atgactgctg tttgtggtgc ggtgggcgct gaaggcatgc actctatgac cgagcagcaa
     6721 gctcgccgtg ctcgcgcttg gatggaggaa gaaatggctg cgccagctgt ggaagcggag
     6781 tatgaggttg ttgacgaggg cgaggtgttt taaggatcca ggagaataca catgccaatt
     6841 caggtcttac cgccacaact ggcgaaccag attgccgcag gtgaggtggt cgagcgacct
     6901 gcgtcggtag tcaaagaact agtgaaaaac agcctcgatg caggtgcgac gcgtatcgat
     6961 attgatatcg aacgcggtgg ggcgaaactt atccgcattc gtgataacgg ctgcggtatc
     7021 aaaaaagatg agctggcgct ggcgctggct cgtcatgcca ccagtaaaat cgcctctctg
     7081 gacgatctcg aagccattat cagcctgggc tttcgcggtg aggcgctggc gagtatcagt
     7141 tcggtttccc gcctgacgct cacttcacgc accgcagaac agcaggaagc ctggcaggcc
     7201 tatgccgaag ggcgcgatat gaacgtgacg gtaaaaccgg cggcgcatcc tgtggggacg
     7261 acgctggagg tgctggatct gttctacaac accccggcgc ggcgcaaatt cctgcgcacc
     7321 gagaaaaccg aatttaacca cattgatgag atcatccgcc gcattgcgct ggcgcgtttc
     7381 gacgtcacga tcaacctgtc gcataacggt aaaattgtgc gtcagtaccg cgcagtgccg
     7441 gaaggcgggc aaaaagaacg gcgcttaggc gcgatttgcg gcaccgcttt tcttgaacaa
     7501 gcgctggcga ttgaatggca acacggcgat ctcacgctac gcggctgggt ggccgatcca
     7561 aatcacacca cgcccgcact ggcagaaatt cagtattgct acgtgaacgg tcgcatgatg
     7621 cgcgatcgcc tgatcaatca cgcgatccgc caggcctgcg aagacaaact gggggccgat
     7681 cagcaaccgg catttgtgtt gtatctggag atcgacccac atcaggtgga cgtcaacgtg
     7741 caccccgcca aacacgaagt gcgtttccat cagtcgcgtc tggtgcatga ttttatctat
     7801 cagggcgtgc tgagcgtgct acaacagcaa ctggaaacgc cgctaccgct ggacgatgaa
     7861 ccccaacctg caccgcgttc cattccggaa aaccgcgtgg cggcggggcg caatcacttt
     7921 gcagaaccgg cagctcgtga gccggtagct ccgcgctaca ctcctgcgcc agcatcaggc
     7981 agtcgtccgg ctgccccctg gccgaatgcg cagccaggct accagaaaca gcaaggtgaa
     8041 gtgtatcgcc agcttttgca aacgcccgcg ccgatgcaaa aattaaaagc gccggaaccg
     8101 caggaacctg cacttgcggc gaacagtcag agttttggtc gggtactgac tatcgtccat
     8161 tccgactgtg cgttgctgga gcgcgacggc aacatttcac ttttatcctt gccagtggca
     8221 gaacgttggc tgcgtcaggc acaattgacg ccgggtgaag cgcccgtttg cgcccagccg
     8281 ctgctgattc cgttgcggct aaaagtttct gccgaagaaa aatcggcatt agaaaaagcg
     8341 cagtctgccc tggcggaatt gggtattgat ttccagtcag atgcacagca tgtgaccatc
     8401 agggcagtgc ctttaccctt acgccaacaa aatttacaaa tcttgattcc tgaactgata
     8461 ggctacctgg cgaagcagtc cgtattcgaa cctggcaata ttgcgcagtg gattgcacga
     8521 aatctgatga gcgaacatgc gcagtggtca atggcacagg ccataaccct gctggcggac
     8581 gtggaacggt tatgtccgca acttgtgaaa acgccgccgg gtggtctgtt acaatctgtt
     8641 gatttacatc cggcgataaa agccctgaaa gatgagtgat ctagagtcga cctgcaggca
     8701 tgcaagcttg cggccgcgtc gtgactggga aaaccctggc gactagtctt ggactcctgt
     8761 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     8821 gccgggcgtt ttttattggt gagaatccag gggtccccaa taattacgat ttaaatttgt
     8881 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     8941 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc agcgtgaaac
     9001 gagctgtagc cgtccgcgtc tgaacagcaa catggatgcg gatctgtatg gctataaatg
     9061 ggcgcgtgat aacgtgggtc agagcggcgc gaccatttat cgtctgtatg gcaaaccgga
     9121 tgcgccggaa ctgtttctga aacatggcaa aggcagcgtg gcgaacgatg tgaccgatga
     9181 aatggtgcgt ctgaactggc tgaccgaatt tatgccgctg ccgaccatta aacattttat
     9241 tcgcaccccg gatgatgcgt ggctgctgac caccgcgatt ccgggcaaaa ccgcgtttca
     9301 ggtgctggaa gaatatccgg atagcggcga aaacattgtg gatgcgctgg ccgtgtttct
     9361 gcgtcgtctg catagcattc cggtgtgcaa ctgcccgttt aacagcgatc gtgtgtttcg
     9421 tctggcccag gcgcagagcc gtatgaacaa cggcctggtg gatgcgagcg attttgatga
     9481 tgaacgtaac ggctggccgg tggaacaggt gtggaaagaa atgcataaac tgctgccgtt
     9541 tagcccggat agcgtggtga cccacggcga ttttagcctg gataacctga ttttcgatga
     9601 aggcaaactg attggctgca ttgatgtggg ccgtgtgggc attgcggatc gttatcagga
     9661 tctggccatt ctgtggaact gcctgggcga atttagcccg agcctgcaaa aacgtctgtt
     9721 tcagaaatat ggcattgata atccggatat gaacaaactg caatttcatc tgatgctgga
     9781 tgaatttttc taataattaa ttggaccgcg gtccgcgcgt tgtccttttc cgctgcataa
     9841 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     9901 acagg
//
