LOCUS       sequence-273018-        5111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Desulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, 
            specR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Keller KL, Bender KS, Wall JD
  TITLE     Development of a markerless genetic exchange system for 
            Desulfovibrio vulgaris Hildenborough and its use in generating a 
            strain with increased transformation efficiency.
  JOURNAL   Appl Environ Microbiol. 2009 Dec;75(24):7682-91. doi: 
            10.1128/AEM.01839-09. Epub 2009 Oct 16.
  PUBMED    19837844
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.01839-09"; journalName: "Appl Environ Microbiol"; date:
            "2009-12"; volume: "75"; issue: "24"; pages: "7682-91"
COMMENT     Sequence Label: pMO719
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2990..3089)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3106..3125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3107..3125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3130..3146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3130..3146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3577..4368
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4461..5049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4950..4969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcccct cggagccagc atcgtaatga ccgaaggccg aagcaagcga
      721 agaggtcaag ggcagaagcg aagcgtccct tgaccggatg agcggcgtag gcagaaggga
      781 atggagagga tgactacgaa ggggaattaa ttccccttgg tggagttgag tggttttaaa
      841 aactggagag gagtccatga aacgaaagca gctgaggatt gaaacgaagt aacagcgcat
      901 actaattgta ttttttgggt gggggaggag ccttgcgacg ggcggcgagc gtagcgaggg
      961 gcctctatag cgcgcgcata cgcgcgcgcg cgcgcatacg cgcgcgcttc ttcttttctt
     1021 tcttttctct tttccccctc gtttttcgtc agttatttcg gcaacctaca gacctaaaag
     1081 tgaccaaaag tacgtctaaa ggtgaccaaa agtacgtcta aaggtgacca aaagtacgtc
     1141 taaaggtgac caaaagtacg tggtgtagcc tttgggtgac caatttgaca agtggtcacc
     1201 tttagccata aaggcaaaag aacagcccga aaaggtgcgg taacacctca acgggcctaa
     1261 cctctcacaa gccctaaaat ggagggtctg caatggttga caggcaatta accttacccg
     1321 gcgagatagt caaaaagtca aacgcactgg ctagggccaa gtggtcccct aagtccgtgt
     1381 gggaaccgcg catggtcgcg ctggtagctt ccatggtccg tcaggacgat gcggactttc
     1441 aggaataccg ctttcctgtc tccacccttt gcgatgagca agagctatcc ggcaagaggt
     1501 acaaggacat taaggccgct gtagaaaatg tgatgaaagc ctatgtgatg attgaggaag
     1561 gcaaaaatta cgtggcgtat agcatcttct caaagtgtgg ctatgaaaac ggtgtcatgg
     1621 tcgccgcctt ccatcccgat ctgaaacccc attttctcgg ccttcaaaag cagttcacaa
     1681 ggtatagcct catggaattt ctggtgctgc ctagcaccta cagccaaagg gttttcgagg
     1741 ttttgaggtc gtgggacgac aagccggaag tgaccatttc acttgtcgat cttcatgaaa
     1801 tggtgggtac gcctccttct ctgcggaaga agtaccccga ctttcgccgt aaggttcttg
     1861 agaaggccca caaggacata aaccgccata ccgcgcttgc ttacgagtgg gaagccgtga
     1921 agaacgggcg ctcggtcgcg ggtatccgct tcctgttcgc gcagaagcgg gcgggcaggg
     1981 tcgtagccaa gaaacaggcc aaggtcgtgc aggacagcag cgacgccaag aacaaggcct
     2041 tcatcgccgc gaccaagtgc cacgcggcca gcaagggccg ctgcacgccg aaagacaacc
     2101 tccagtgcca agtgtgcgcc cagctcgtca gcctgggcaa gagagccgga acagcctaaa
     2161 cctcttttgt agcacccccc ctttgaggct gtagcacccc ccttttgagg ctgtagcacc
     2221 ttgtgacgtg cctgtagcac cttttcagac ttcacatttt taaccataac gtgctgtttt
     2281 aacagaaatt ttctttacgt ttcagcaccg gctgatctcg cttttttgtg ccacaaaaag
     2341 gctatcctgc caagcggcat taaaacaagc cacctcatca acaaaatgaa ggggggcgcg
     2401 gcccgctcac atggttgcag gtgaagaaga ttggaaatcg aggcgaaggt tattccattc
     2461 cccgcagtag aacctgaaca gggaagcctg ttctaatagc cctaaaatcg attttaagcg
     2521 caggaacaag caagaagaga ctcaggctca ccgagggaaa tttcctccct tacgccccct
     2581 tctacgcttc gttatcgcta cgccgaaaaa aagagcaagg gacgcttcgc ttctgccctt
     2641 gctctttttt tgcgccttcg ctgcactccg ctcacgaagg cggctccggg ggaaatttca
     2701 gcctggttcc aacgacaagc gaagcgggat ctgcgggggg cccccgtgag ggggggcggc
     2761 tagccccatc ccctgcttgc aggggcagga ccggcgtagc cggagcgagt ggtgatggtc
     2821 ttgtgatcgt ccagagtgat ttccgttgtg ctggactctc ccgattctcg acgagctgaa
     2881 agcgagaaga gcgcacttat acatcgctgc gcgacgtgtg cgccctgcgg ggctaagtca
     2941 tcggaacaaa agagtttccc cgtagtgggg aaaagggggc ttgaattcga cccagctttc
     3001 ttgtacaaag ttggcattat aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc
     3061 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     3121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     3181 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
     3241 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     3301 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     3361 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     3421 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     3481 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     3541 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     3601 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     3661 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     3721 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     3781 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     3841 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     3901 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     3961 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     4021 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     4081 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     4141 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     4201 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     4261 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     4321 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
     4381 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     4441 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4501 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4561 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4621 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4681 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4741 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4801 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4861 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4921 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4981 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5041 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5101 gctcacatgt t
//
