LOCUS       sequence-273022-       10031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible codon-optimized mouse Dux.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Fu X, Wu X, Djekidel MN, Zhang Y
  TITLE     Myc and Dnmt1 impede the pluripotent to totipotent state transition 
            in embryonic stem cells.
  JOURNAL   Nat Cell Biol. 2019 Jul;21(7):835-844. doi: 
            10.1038/s41556-019-0343-0. Epub 2019 Jun 17.
  PUBMED    31209294
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-019-0343-0"; journalName: "Nat Cell Biol"; date: 
            "2019-07"; volume: "21"; issue: "7"; pages: "835-844"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2-mDux
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..36
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..88
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2168..2224
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        2252..2762
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2681..2699
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(2786..3403,3404..3409,3410..3445,3446..3448,3449..3484,
                     3485..3487,3488..3523,3524..3529)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             3545..3598
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3599..4402
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3662..3681)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4272..4291
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4443..4463)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4861..4872)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5077..5310
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5382..5503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5419..5438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5473..5492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5543..5678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5678..5749
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5750..5818,5819..6610)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5968..5987)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6781..7369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7270..7289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7523..7540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7657..7678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7693..7698,7699..7716,7717..7723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7731..7747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7736..7758
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7755..7771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7755..7771
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7790..7810
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7792..7810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7838..8064
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8065..8245
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8292..8417
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8910..9143
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9328..9372
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9585..9702
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9756..10031,1..39)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9764..9782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9800..9818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9835..9853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9871..9889
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9907..9925
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9942..9960
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9978..9996
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagaattgg ctagcgccac
       61 catggactac aaagacgatg acgacaaggg atccactagt aacggccgcc agtgtgctgg
      121 aattctggct gaagctgggt ctcctgtggg aggctccgga gttgccagag agtctagaag
      181 gaggaggaag actgtgtggc aagcttggca ggagcaggcc ttgttgagta ccttcaaaaa
      241 gaagagatat ctctctttta aggaaaggaa ggagttggcc aaaaggatgg gggtatctga
      301 ctgcaggatc agagtatggt tccaaaatag gcgaaacaga tcaggagagg aagggcatgc
      361 ttcaaagagg agcataagag gatccaggcg actggcctca ccccagctcc aggaagaatt
      421 ggggagtaga cctcagggca gaggcatgag atccagcggg agaaggccta gaactagact
      481 gacaagtctc cagctcagga tcttggggca agcatttgag agaaatccta gaccagggtt
      541 tgcaaccagg gaggagcttg ctagggacac cggcctgcca gaggacacta ttcatatatg
      601 gtttcagaat agaagggctc gaagaaggca caggagggga agacctaccg cccaggatca
      661 ggacctcttg gctagtcaag gatctgatgg tgcaccagcc ggcccagaag gaagagaaag
      721 agagggagct caagaaaatc ttcttcccca agaagaggca ggaagcacag gaatggacac
      781 atcctctcca tctgatcttc ctagcttttg tggtgaatcc caacccttcc aagtagcaca
      841 accaagggga gccggacaac aagaagcacc tacaagggca gggaatgctg gatccctcga
      901 accattgctg gaccagttgc ttgatgaggt gcaagttgag gaaccagcac ctgcaccact
      961 gaacctggac ggagatcctg gtgggagagt acatgaaggg tcccaagaga gcttctggcc
     1021 acaagaagag gcaggttcaa ctggcatgga taccagttct ccttctgatt ctaattcttt
     1081 ttgtagggag agccaaccaa gccaggtggc ccaaccttgt ggtgctgggc aggaggatgc
     1141 aaggactcaa gctgattcaa caggcccact tgaactgttg ttgctggacc aactgctgga
     1201 tgaagttcag aaagaagagc atgtcccagt accacttgac tggggtagaa atccaggaag
     1261 tagagaacac gagggcagtc aagactcact gcttcctctg gaagaggccg ttaatagtgg
     1321 gatggataca agtatcccat caatatggcc tactttttgt agagagtcac agccaccaca
     1381 ggtcgcacaa ccatctgggc ctggacaggc acaggcacca actcaaggag ggaatactga
     1441 cccattggag ttgttccttt atcaacttct ggatgaagtg caggtggagg aacatgcacc
     1501 tgctcccctc aactgggatg tggatcctgg tggaagagtg catgaggggt cttgggagag
     1561 cttctggcca caggaagaag caggaagtac tggccttgat acctcatccc cttctgattc
     1621 caattcattc tttagagagt ctaaaccttc tcaagtagcc caaaggagag gtgctggcca
     1681 ggaggatgca aggacacagg ctgattctac cggacctctg gagctgctgc tctttgacca
     1741 actgctggac gaggtgcaga aagaggaaca cgtccctgcc ccccttgact ggggaaggaa
     1801 tcccggtagc atggagcatg aaggttccca ggacagcctg ctccctctgg aggaagctgc
     1861 taattccggc agagatacct caatacctag tatctggccc gctttctgca gaaagtcaca
     1921 acccccacaa gtggcacagc catcaggacc tggccaggca caagcaccta tacaaggagg
     1981 taatacagat cccctggagt tgtttctgga tcagcttctt actgaagtac agttggagga
     2041 gcagggccct gctcctgtga acgtggaaga aacatgggag caaatggaca ccacacctga
     2101 tttgcccttg acctctgagg agtaccagac tcttctggac atgttgtgag tcgacaccgg
     2161 ttccggagcc acgaacttct ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg
     2221 tcctaggctg cagacgcgtt gtacaggatc cggggttggg gttgcgcctt ttccaaggca
     2281 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     2341 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     2401 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     2461 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     2521 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     2581 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     2641 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     2701 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     2761 agcaattcac ccgcgcgcgg ccaccatgtc tagactggac aagagcaaag tcataaacgg
     2821 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     2881 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     2941 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     3001 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     3061 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     3121 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     3181 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     3241 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     3301 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     3361 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     3421 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     3481 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt tcgaaggaag
     3541 cggagaaggt agaggttctc tcctcacttg tggtgatgtt gaagaaaacc ctggtccaat
     3601 gtctagaatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3661 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     3721 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     3781 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     3841 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     3901 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     3961 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4021 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4081 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     4141 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4201 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4261 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4321 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4381 ccttcttgac gagttcttct gaatgcattg aggatcgatc caagatatcg tattcttaac
     4441 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4501 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4561 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4621 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4681 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4741 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4801 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4861 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4921 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4981 gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg
     5041 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5101 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5281 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     5341 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     5401 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5461 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     5521 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5581 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5641 gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt atccgctcat
     5701 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5761 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5821 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5881 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5941 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6001 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6061 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6121 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6181 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6241 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6301 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6361 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6421 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6481 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6541 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6601 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6661 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6721 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6781 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6841 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6901 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6961 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7021 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7081 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7141 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7201 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7261 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7321 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7381 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7441 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7501 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7801 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     7861 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7921 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     7981 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     8041 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     8101 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8161 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8221 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     8281 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     8341 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     8401 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     8461 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     8521 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8581 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8641 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8701 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     8761 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8821 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8881 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8941 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     9001 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     9061 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     9121 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     9181 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     9241 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     9301 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     9361 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     9421 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     9481 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9541 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9601 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9661 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9721 agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat cagtgataga
     9781 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     9841 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     9901 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     9961 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
    10021 ggtgggaggc c
//
