LOCUS       sequence-273023-        4837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty complementation vector for Desulfovibrio: contains promoter 
            from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, 
            SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Keller KL, Wall JD, Chhabra S
  TITLE     Methods for engineering sulfate reducing bacteria of the genus 
            Desulfovibrio.
  JOURNAL   Methods Enzymol. 2011;497:503-17. doi: 
            10.1016/B978-0-12-385075-1.00022-6.
  PUBMED    21601101
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2011"; volume: "497:503-17. doi"; pages: " 
            10.1016/B978-0-12-385075-1.00022-6"
COMMENT     Sequence Label: pMO9075
FEATURES             Location/Qualifiers
     source          1..4837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2832..2851)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2833..2851)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2856..2872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2856..2872)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3303..4094
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4187..4775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4676..4695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taattcccct tggtggagtt gagtggtttt aaaaactgga
      541 gaggagtcca tgaaacgaaa gcagctgagg attgaaacga agtaacagcg catactaatt
      601 gtattttttg ggtgggggag gagccttgcg acgggcggcg agcgtagcga ggggcctcta
      661 tagcgcgcgc atacgcgcgc gcgcgcgcat acgcgcgcgc ttcttctttt ctttcttttc
      721 tcttttcccc ctcgtttttc gtcagttatt tcggcaacct acagacctaa aagtgaccaa
      781 aagtacgtct aaaggtgacc aaaagtacgt ctaaaggtga ccaaaagtac gtctaaaggt
      841 gaccaaaagt acgtggtgta gcctttgggt gaccaatttg acaagtggtc acctttagcc
      901 ataaaggcaa aagaacagcc cgaaaaggtg cggtaacacc tcaacgggcc taacctctca
      961 caagccctaa aatggagggt ctgcaatggt tgacaggcaa ttaaccttac ccggcgagat
     1021 agtcaaaaag tcaaacgcac tggctagggc caagtggtcc cctaagtccg tgtgggaacc
     1081 gcgcatggtc gcgctggtag cttccatggt ccgtcaggac gatgcggact ttcaggaata
     1141 ccgctttcct gtctccaccc tttgcgatga gcaagagcta tccggcaaga ggtacaagga
     1201 cattaaggcc gctgtagaaa atgtgatgaa agcctatgtg atgattgagg aaggcaaaaa
     1261 ttacgtggcg tatagcatct tctcaaagtg tggctatgaa aacggtgtca tggtcgccgc
     1321 cttccatccc gatctgaaac cccattttct cggccttcaa aagcagttca caaggtatag
     1381 cctcatggaa tttctggtgc tgcctagcac ctacagccaa agggttttcg aggttttgag
     1441 gtcgtgggac gacaagccgg aagtgaccat ttcacttgtc gatcttcatg aaatggtggg
     1501 tacgcctcct tctctgcgga agaagtaccc cgactttcgc cgtaaggttc ttgagaaggc
     1561 ccacaaggac ataaaccgcc ataccgcgct tgcttacgag tgggaagccg tgaagaacgg
     1621 gcgctcggtc gcgggtatcc gcttcctgtt cgcgcagaag cgggcgggca gggtcgtagc
     1681 caagaaacag gccaaggtcg tgcaggacag cagcgacgcc aagaacaagg ccttcatcgc
     1741 cgcgaccaag tgccacgcgg ccagcaaggg ccgctgcacg ccgaaagaca acctccagtg
     1801 ccaagtgtgc gcccagctcg tcagcctggg caagagagcc ggaacagcct aaacctcttt
     1861 tgtagcaccc cccctttgag gctgtagcac cccccttttg aggctgtagc accttgtgac
     1921 gtgcctgtag caccttttca gacttcacat ttttaaccat aacgtgctgt tttaacagaa
     1981 attttcttta cgtttcagca ccggctgatc tcgctttttt gtgccacaaa aaggctatcc
     2041 tgccaagcgg cattaaaaca agccacctca tcaacaaaat gaaggggggc gcggcccgct
     2101 cacatggttg caggtgaaga agattggaaa tcgaggcgaa ggttattcca ttccccgcag
     2161 tagaacctga acagggaagc ctgttctaat agccctaaaa tcgattttaa gcgcaggaac
     2221 aagcaagaag agactcaggc tcaccgaggg aaatttcctc ccttacgccc ccttctacgc
     2281 ttcgttatcg ctacgccgaa aaaaagagca agggacgctt cgcttctgcc cttgctcttt
     2341 ttttgcgcct tcgctgcact ccgctcacga aggcggctcc gggggaaatt tcagcctggt
     2401 tccaacgaca agcgaagcgg gatctgcggg gggcccccgt gagggggggc ggctagcccc
     2461 atcccctgct tgcaggggca ggaccggcgt agccggagcg agtggtgatg gtcttgtgat
     2521 cgtccagagt gatttccgtt gtgctggact ctcccgattc tcgacgagct gaaagcgaga
     2581 agagcgcact tatacatcgc tgcgcgacgt gtgcgccctg cggggctaag tcatcggaac
     2641 aaaagagttt ccccgtagtg gggaaaaggg ggcttgaatt cgcccttgca tgcgtttaaa
     2701 ctacgtaagg agtactgcgc agatcgtgat cccctgcgcc atcagatcct tggcggcaag
     2761 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
     2821 aattccggat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     2881 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     2941 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     3001 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     3061 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     3121 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     3181 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     3241 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     3301 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     3361 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     3421 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     3481 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     3541 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     3601 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     3661 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     3721 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     3781 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     3841 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     3901 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     3961 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     4021 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     4081 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     4141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4801 ggttcctggc cttttgctgg ccttttgctc acatgtt
//
