LOCUS       sequence-273037-        5799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 548 / 634, 
            Emission: 565 / 662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5799)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-mOrange2-Homer1-N-18
FEATURES             Location/Qualifiers
     source          1..5799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        378..581
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     532..552
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      610..619
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1762..1771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1768..2475
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     2375..2398
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2600..2721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2637..2656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2691..2710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2728..3183)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2865..2886)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3077..3096
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3210..3314
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3316..3673
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3316..3507
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3339..3359)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3524..3659
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3708..4502
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3708..4502
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3762..3781)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4372..4391
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4690..4709)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4734..4781
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5110..5698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5599..5618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
       61 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      121 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      181 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      241 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      301 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      361 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      421 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      481 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      541 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
      601 tccgctagcg ccaccatggg ggaacaacct atcttcagca ctcgagctca tgtcttccag
      661 atcgacccaa acacaaagaa gaactgggta cccaccagca agcatgcagt tactgtgtct
      721 tatttctatg acagcacaag gaatgtgtat aggataatca gtttagacgg ctcaaaggca
      781 ataataaata gcaccatcac tccaaacatg acatttacta aaacatctca aaagtttggc
      841 caatgggctg atagccgggc aaacactgtt tatggactgg gattctcctc tgagcatcat
      901 ctctcaaaat ttgcagaaaa gtttcaggaa tttaaagaag ctgctcggct ggcaaaggag
      961 aagtcgcagg agaagatgga actgaccagt accccttcac aggaatcagc aggaggagat
     1021 cttcagtctc ctttaacacc agaaagtatc aatgggacag atgatgagag aacacccgat
     1081 gtgacacaga actcagagcc aagggctgag ccagctcaga atgcattgcc attttcacat
     1141 agtgccgggg atcgaaccca gggcctctct catgctagtt cagccatcag caaacactgg
     1201 gaggctgaac tagccacgct caaggggaac aatgccaagc tcaccgcagc gctgctggag
     1261 tccactgcca acgtgaagca gtggaagcaa cagctggctg cctaccagga ggaggcagag
     1321 cggctgcaca agcgggtcac ggagctggaa tgtgttagta gtcaagcaaa cgcggtgcac
     1381 agccacaaga cagagctgag tcagacagtg caggagctgg aagagaccct aaaagtaaag
     1441 gaagaggaaa tagaaagatt aaaacaagaa attgataacg ccagagaact tcaagaacag
     1501 agggactctt tgactcagaa actacaggaa gttgagattc gaaataaaga cctggagggg
     1561 cagctgtcgg agctggagca gcgcctggag aagagccaga gcgagcagga cgctttccgc
     1621 agtaacctga agactctcct agagattctg gacgggaaaa tatttgaact aacagaattg
     1681 cgggataatt tggccaagct actagaatgc agcgggtcca gcggttctag cggctccggg
     1741 agtggagggg atccaccggt cgccaccatg gtgagcaagg gcgaggagaa taatatggcc
     1801 atcattaagg agttcatgcg cttcaaggtg cacatggagg gcactgtgaa cggccacgag
     1861 ttcgagatcg agggcgaggg cgagggccac ccctacgagg gctttcagac cgctaagctg
     1921 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc tctcatcacc
     1981 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt caagctgtcc
     2041 ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg aggacggcgg cgtggtgacc
     2101 gtgacccagg actcctctct gcaggacggc gagttcatct acaaggtgaa gatgcgcggc
     2161 accaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc
     2221 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaggat gaggctgaag
     2281 ctgaaggatg gcggccacta cacctccgag gtcaagacta cctacaaggc caagaagtcc
     2341 gtgctgctgc ccggcgccta catcgtcggc atcaagctgg acatcacctc ccacaacgag
     2401 gactacacca tcgtggaaca gtacgaacgc tccgaggccc gccactccac cggcggcatg
     2461 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     2521 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2581 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2701 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     2761 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2821 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     2881 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     2941 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3001 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3061 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3121 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3181 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3241 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3301 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     3361 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3421 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3481 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3541 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3601 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3661 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3721 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3781 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3841 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     3901 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3961 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4021 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4081 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4141 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4201 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     4261 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     4321 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4381 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4441 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4501 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     4561 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4621 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     4681 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     4741 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     4801 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     4861 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     4921 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     4981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5221 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5761 ctttcctgcg ttatcccctg attctgtgga taaccgtat
//
