LOCUS       sequence-273041-        5188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P3g.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, 
            Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR
  TITLE     Phage-assisted evolution of an adenine base editor with improved Cas
            domain compatibility and activity.
  JOURNAL   Nat Biotechnol. 2020 Jul;38(7):883-891. doi: 
            10.1038/s41587-020-0453-z. Epub 2020 Mar 16.
  PUBMED    32433547
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0453-z"; journalName: "Nat Biotechnol"; date: 
            "2020-07"; volume: "38"; issue: "7"; pages: "883-891"
COMMENT     Sequence Label: pBT138b-proA
FEATURES             Location/Qualifiers
     source          1..5188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2839..3630)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3631..3702)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3730..4318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4219..4238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4472..4489
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4504..4644
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4570..4589)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4730..4752
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4746..4937)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cataaggctc gtaggctata ttcagggaga ccacaacggt ttccctctac aaataatttt
       61 gtttaacttt tactagagtg ggaccctacc tgcaggtgca gtactacatc atcaggcatg
      121 aacacgatta acatcgctaa gaacgacttc tctgacatcg aactggctgc tatcccgttc
      181 aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc ccttgagcat
      241 gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagtgata acttaaagct
      301 ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact ccctaagatg
      361 attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa gcgcccgaca
      421 gccttccagt tcctgcaaga aatcaagccg gaagccgtag cgtacatcac cattaagacc
      481 actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc aagcgcaatc
      541 ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttaa agctaagcac
      601 ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta caagaaagca
      661 tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg cgaggcgtgg
      721 tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga gatgctcatt
      781 gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg tcaagactct
      841 gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc aggtgcgctg
      901 gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg gactggcatt
      961 actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg tactcacagt
     1021 aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa agcgattaac
     1081 attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc caacgtaatc
     1141 accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga agaactcccg
     1201 atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa acgtgctgcc
     1261 gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct tgagttcatg
     1321 cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta caacatggac
     1381 tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga tatgaccaaa
     1441 ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta ctggctgaaa
     1501 atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg catcaagttc
     1561 attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga gaacacttgg
     1621 tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta cgctggggta
     1681 cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg gtcttgctct
     1741 ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc ggttaacttg
     1801 cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt caacgagatt
     1861 ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac cgatgagaac
     1921 actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg tcaatggctg
     1981 gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc ttacgggtcc
     2041 aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc tattgattcc
     2101 ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc taagctgatt
     2161 tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg gcttaagtct
     2221 gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat tcttcgcaag
     2281 cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga atacaagaag
     2341 cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca gcctaccatt
     2401 aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat cgctcctaac
     2461 tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc acacgagaag
     2521 tacggtatcg aatcttttgc actgattcac gactccttcg gtaccattcc ggctgacgct
     2581 gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc ttgtgatgta
     2641 ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt ggacaaaatg
     2701 ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc cgacttcgcg
     2761 ttcgcctaat ggcactcctc agcaaatata atgaccctct tgataaccca agagggcatt
     2821 ttttaatgcc catggcgttt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     2881 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     2941 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     3001 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     3061 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     3121 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     3181 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     3241 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     3301 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     3361 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     3421 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     3481 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     3541 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     3601 cgatacttcg gcgatcaccg cttccctcat actcttcctt tttcaatatt attgaagcat
     3661 ttatcagggt tattgtctca tgagcgtata catatttgaa tgtatttaga aaaataggcc
     3721 aaatagggtt tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3781 accgctacca gcggtggttt gtttgccgga tcaagagcta ccacctcttt ttccgaaggt
     3841 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3901 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3961 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4021 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4081 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4141 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4201 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4261 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4321 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4381 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4441 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggatga
     4501 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     4561 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     4621 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     4681 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4741 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
     4801 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
     4861 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     4921 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     4981 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acaagaggac
     5041 atccggtcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttgctgagga
     5101 gacttaggga ctctcacagc taacaccacg tcgtccctat ctgctgccct aggtctatga
     5161 gtggttgctg gataacttta cgggcatg
//
