LOCUS       sequence-27305-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: FLAG-Ago1
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..117
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    130..154
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgcgagtca tatagcagag ctcgtttagt gaaccgtcag aattaaccat ggactacaaa
       61 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagctt
      121 gcggccatca caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
      181 atccggtacc taggcccctc acgctggact tcacagtctc cgggccgcct gacctccgca
      241 cgggtatatg ggatggaagc gggaccctcg ggagcagctg cgggcgctta cctgcccccc
      301 ctgcagcagg tgttccaggc acctcgccgg cctggcattg gcactgtggg gaaaccaatc
      361 aagctcctgg ccaattactt tgaggtggac atccctaaga tcgacgtgta ccactacgag
      421 gtggacatca agccggataa gtgtccccgt agagtcaacc gggaagtggt ggaatacatg
      481 gtccagcatt tcaagcctca gatctttggt gatcgcaagc ctgtgtatga tggaaagaag
      541 aacatttaca ctgtcacagc actgcccatt ggcaacgaac gggtcgactt tgaggtgaca
      601 atccctgggg aagggaagga tnnnnnaatc tttaaggtct ccatcaagtg gctagccatt
      661 gtgagctggc gnaatgctgc atgaggccct ggtcagcggc cagatccctg ttcccttgga
      721 gtctgtgcaa gccctggatg tggccatgag gcacctggca tccatgaggt acacccctgt
      781 gggccgctcc ttcttctcac cgcctgaggg ctactaccac ccgctggggg gtgggcgcga
      841 gg
//
