LOCUS       sequence-27309-e         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: NHA-Ago4
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          124..256
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     239..258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        301..319
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      323..332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             329..394
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             431..457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             437..463
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    515..539
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggtagttta tcacagttaa attgctaacg cagtcagtgc ttctgacaca acagtctcga
       61 acttaagctg cagtgactct cttaaggtag ccttgcagaa gttggtcgtg aggcactggg
      121 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
      181 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
      241 gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg ctagagtact
      301 taatacgact cactataggc tagccaccat ggacgcacaa acacgacgac gtgagcgtcg
      361 cgctgagaaa caagctcaat ggaaagctgc aaacccaccg ctcgaggctg cccaagctta
      421 caccatggcc tacccatatg atgttccaga ttacgcttca ctcgacgcgg atcccgtcga
      481 attcacgcgt ggtacctcta gagtcgaccc atcaacaagt ttgtacaaaa aagcaggctt
      541 tgggccgggc gccgccgccg ccccctgccc agcgcccgcg tctccgcgg
//
