LOCUS       sequence-273104-        3966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Atz reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7030
FEATURES             Location/Qualifiers
     source          1..3966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..63
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(101..123)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     223..242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     436..458
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     450..467
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     451..467
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             652..1365
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITTDKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(682..710)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1305..1325
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1388..1417
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1433..1450
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1506..1523)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1676..1722
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1818..1834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1818..1834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1831..1853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1842..1858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1866..1872,1873..1890,1891..1896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1911..1932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2049..2066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2219..2807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2299..2318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2978..3769,3770..3838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3601..3620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3839..3943)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
       61 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      121 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      181 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
      241 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      301 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      361 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      421 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtaag atctcggctg
      481 aattctaaag atctcatgcg agtcaaagca agatcggtgc cggatcggca ccagttaggt
      541 cggaaaaagg cggcagtcaa gtgcgcaggg cggcgttata attgaacgaa acgtcgactc
      601 tcgagtgaga ttgttgacgg taccgtattt tggatctagg aggaaggatc tatgagcaaa
      661 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      721 gggcacaaat tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc
      781 cttaaattta tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact
      841 ctgacctatg gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt
      901 ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac
      961 gggacctaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc
     1021 gagttaaagg gtattgattt taaagaagat ggaaacattc ttggacacaa actcgagtac
     1081 aactttaact cacacaatgt atacatcacg acagacaaac aaaagaatgg aatcaaagct
     1141 aacttcaaaa ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa
     1201 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
     1261 caatctgtcc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt
     1321 gtaactgctg ctgggattac acatggcatg gatgagctct acaaataagg atctgaagct
     1381 tgggcccgaa caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca
     1441 tcatcatcat tgagtttaaa cggtctccag cttggctgtt ttggtggatg agagaagatt
     1501 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
     1561 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
     1621 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
     1681 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1741 ctctgaaagg aggaactata tccggatcga gatcctctag agtcgacctg caggcatgca
     1801 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1861 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1921 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1981 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2041 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2161 atgtgagcaa aaggccagca aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2221 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2281 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2341 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2401 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2461 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2521 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2581 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2641 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2701 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2761 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2821 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2881 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3961 tgccac
//
