LOCUS       sequence-27312-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: NHA-PIWI(aa478-860)
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..129
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             109..135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    187..211
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      742..751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggacggcca aacaccgacg acgtgagcgt cgcgctgaga aacaagctca atggaaagct
       61 gcaaacccac cgctcgaggc tgcccaagct tacaccatgg cctacccata tgatgttcca
      121 gattacgctt cactcgacgc ggatcccgtc gaattcacgc gtggtacctc tagagtcgac
      181 ccatcaacaa gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga
      241 tcgatctcga gagacgctgg catgcccatc cagggccagc cgtgcttctg caaatacgcg
      301 cagggggcgg acagcgtgga gcccatgttc cggcacctga agaacacgta tgcgggcctg
      361 cagctggtgg tggtcatcct gcccggcaag acgcccgtgt acgccgaggt caagcgcgtg
      421 ggagacacgg tgctggggat ggccacgcag tgcgtgcaga tgaagaacgt gcagaggacc
      481 acgccacaga ccctgtccaa cctctgcctg aagatcaacg tcaagctggg aggcgtgaac
      541 aacatcctgc tgccccaggg caggccgccg gtgttccagc agcccgtcat ctttctggga
      601 gcagacgtca ctcacccccc cgccggggat gggaagaagc cctccattgc cgccgtggtg
      661 ggcagcatgg acgcccaccc caatcgctac tgcgccaccg tgcgcgtgca gcagcaccgg
      721 caggagatca tacaagacct ggccgccatg gtccgcgagc tcctcatc
//
