LOCUS       sequence-273121-        3445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes constitutive yellow (sfYFP) fluorescence for identification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Alnahhas RN, Winkle JJ, Hirning AJ, Karamched B, Ott W, Josic K, 
            Bennett MR
  TITLE     Spatiotemporal Dynamics of Synthetic Microbial Consortia in 
            Microfluidic Devices.
  JOURNAL   ACS Synth Biol. 2019 Sep 20;8(9):2051-2058. doi: 
            10.1021/acssynbio.9b00146. Epub 2019 Aug 9.
  PUBMED    31361464
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00146"; journalName: "ACS Synth Biol"; date: 
            "2019-09-20- 20"; volume: "8"; issue: "9"; pages: "2051-2058"
COMMENT     Sequence Label: Sender_plasmid1
FEATURES             Location/Qualifiers
     source          1..3445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(193..297)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    338..354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     343..365
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             556..585
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             838..1551
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1737..1764
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1860..1877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1994..2539)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2074..2093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2653..2747)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2777..3445,1..123,124..192))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3400..3419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
       61 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      121 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      181 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      301 atttccccga aaagtgccac ctgacgtctg tgtggaattg tgagcggata acaatttcac
      361 acagggccct cggacaccga ggagaatgtc aagaggcgaa cacacaacgt cttggagcgc
      421 cagaggagga acgagctaaa acggagcttt tttgccctgc gtgaccagat cccggagttg
      481 gaaaacaatg aaaaggcccc caaggtagtt atccttaaaa aagccacagc atacatcctg
      541 tccgtccaag cagaggagca aaagctcatt tctgaagagg acttgttgcg gaaacgacga
      601 gaacagttga aacacaaact tgaacagcta cggaactctt gtgcgtaagg aaaagtaagg
      661 aaaacgattc cttctaacag aaatgtcctg agcaatcacc tatgaactgt cgatggtgca
      721 aaacctttcg cggtatggca tgatagcgcc cggaagcatg ccacttaaaa aggagatcaa
      781 caatgaaagc aattttcgta ctgaaacatc ttaatcatgc taaggagaaa tactagtatg
      841 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
      901 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
      961 ctgacgctga agttcatctg tactactggt aaactgccgg taccttggcc gactctggta
     1021 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
     1081 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
     1141 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
     1201 cgcattgagc tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg
     1261 gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt
     1321 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
     1381 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
     1441 agctaccaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
     1501 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa agcagcgaac
     1561 gacgaaaatt acgccgcgtc tgtttaataa aagcttcccc tacgtgctgc tgaagttgcc
     1621 cgcaacagag agtggaacca accggtgata ccacgatact atgactgaga gtcaacgcca
     1681 tgagcggcct catttcttat tctgagttac aacagtccgc accgctgtct cacactggct
     1741 caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt
     1801 attaatccgg cttttttatt atttcctagc tagctagacc tagggatata ttccgcttcc
     1861 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa
     1921 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg
     1981 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct
     2041 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg
     2101 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     2161 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     2221 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     2281 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
     2341 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
     2401 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
     2461 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg
     2521 cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc
     2581 agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgtt
     2641 actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     2701 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcgt
     2761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2821 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3181 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3421 gaactttaaa agtgctcatc attgg
//
