LOCUS       sequence-273124-        3821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low Phosphate inducible matB (malonyl-CoA synthetase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, 
            Wang R, Lynch MD
  TITLE     Scalable, two-stage, autoinduction of recombinant protein expression
            in E. coli utilizing phosphate depletion.
  JOURNAL   Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440.
  PUBMED    32441815
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 22. doi: 10.1002/bit.27440."
COMMENT     Sequence Label: pCDF-yibDp-matB
FEATURES             Location/Qualifiers
     source          1..3821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(792..821)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      905..976
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      992..1019
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             1302..1319
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2061..2072)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2938..3729)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRTDQLEEFVHYVKGEITKVVGK"
     promoter        complement(3730..3821)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tagctagctc actcggtcgc tacgctccgg gcgtgagact gcggcgggcg ctgcggacac
       61 atacaaagtt acccacagat tccgtggata agcaggggac taacatgtga ggcaaaacag
      121 cagggccgcg ccggtggcgt ttttccatag gctccgccct cctgccagag ttcacataaa
      181 cagacgcttt tccggtgcat ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac
      241 gggcgaaacc cgacaggact taaagatccc caccgtttcc ggcgggtcgc tccctcttgc
      301 gctctcctgt tccgaccctg ccgtttaccg gatacctgtt ccgcctttct cccttacggg
      361 aagtgtggcg ctttctcata gctcacacac tggtatctcg gctcggtgta ggtcgttcgc
      421 tccaagctgg gctgtaagca agaactcccc gttcagcccg actgctgcgc cttatccggt
      481 aactgttcac ttgagtccaa cccggaaaag cacggtaaaa cgccactggc agcagccatt
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 acctcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgattttcta ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg attgcagtcc
      841 agttacgctg gagtctgagg ctcgtcctga atgatatcaa gcttgaattc gtttgcccag
      901 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      961 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1021 ttatacacag ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct
     1081 ggataacgtg cgtaattgtg ctgatctctt atatagctgc tctcattatc tctctaccct
     1141 gaagtgactc tctcacctgt aaaaataata tctcacaggc ttaatagttt cttaatacaa
     1201 agcctgtaaa acgtcaggat aacttctata ttcagggaga ccacaacggt ttccctctac
     1261 aaataatttt gtttaacttt ggaaaaagga gatataccat gcatcatcat catcatcact
     1321 ccaaccattt attcgatgcg atgcgtgccg cggcgcctgg gaatgcgccg ttcatccgca
     1381 tcgacaatac acgcacctgg acctacgacg atgcctttgc cttgagtggt cgcattgcgt
     1441 cagctatgga cgcactcggc attcgcccgg gagaccgcgt ggccgtgcag gtggagaaat
     1501 ctgccgaagc actgattctg tatcttgcgt gtctgcgtag cggtgccgta tatttgccac
     1561 tgaacactgc ttatacactt gcggaactgg actactttat tggtgatgcc gaaccgcgcc
     1621 tcgttgttgt agcgtcatcc gcccgtgcag gtgtggaaac cattgcgaaa ccgcgcgggg
     1681 ccattgtaga aactctggat gcagctggca gcggaagctt gctggacttg gcgcgcgatg
     1741 agcctgctga tttcgtggac gctagtcgct cggcggacga tctggcggca attctttata
     1801 caagtgggac gacagggcgt tctaaggggg caatgctgac acacggcaac ctgctttcta
     1861 acgcgcttac attgcgtgat ttctggcgtg taaccgcagg cgatcgtctg attcatgcgt
     1921 taccgatttt tcatacacat ggcctgtttg tcgctactaa tgtcacatta ctggccgggg
     1981 cctctatgtt tctgttaagc aaatttgatc cggaagagat cctgtctttg atgccgcagg
     2041 ctaccatgct gatgggcgta ccgaccttct atgttcgctt gctgcaatca ccgcgcctgg
     2101 ataaacaggc agtagcgaat attcgcctgt ttattagtgg gtccgcacca ctgctggcag
     2161 agacacacac tgaatttcaa gcgcgtaccg gccatgccat tctggaacgc tacggaatga
     2221 ccgagaccaa catgaacacc tcaaatccgt atgagggtaa acgtattgcg ggtactgtgg
     2281 gctttcctct cccggatgtc actgttcgtg ttaccgaccc ggcaaccggt ctcgccttac
     2341 ctccggaaca gacgggaatg atcgaaatta aaggtccgaa cgtgtttaag ggctattggc
     2401 gcatgcccga gaagaccgct gccgaattca ccgccgatgg tttctttatc agtggtgatt
     2461 taggtaaaat cgatcgcgat ggatacgttc atattgtggg gcgcgggaaa gatctggtta
     2521 tttcaggagg ctataatatt tatccgaaag aagttgaggg ggaaattgac cagattgaag
     2581 gggtggttga atcagcagtg atcggcgttc cgcacccgga ttttggtgaa ggtgtcacag
     2641 cggttgtggt tcgcaaacca ggggcggctc tggatgaaaa agcgatcgtc tctgctctgc
     2701 aggaccgtct ggctcgttat aaacaaccga aacgcatcat ttttgctgaa gatctgccgc
     2761 gtaacacaat gggtaaagtc cagaaaaaca tcttgcgcca gcagtatgca gacttatata
     2821 ctcgtacgta gtaagacgaa ttctctagat atcgctcaat actgaccatt taaatcatac
     2881 ctgaccgtca tcgtggccgg atcttgcggc ccctcggctt gaacgaattg ttagacatta
     2941 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
     3001 tgtgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
     3061 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
     3121 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
     3181 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
     3241 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     3301 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     3361 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     3421 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
     3481 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
     3541 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
     3601 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
     3661 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
     3721 ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3781 gagcggatac atatttgaat gtatttagaa aaataaacaa a
//
