LOCUS       sequence-273126-        3605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low Phosphate inducible mdlC benzylformate decarboxylase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, 
            Wang R, Lynch MD
  TITLE     Scalable, two-stage, autoinduction of recombinant protein expression
            in E. coli utilizing phosphate depletion.
  JOURNAL   Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440.
  PUBMED    32441815
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 22. doi: 10.1002/bit.27440."
COMMENT     Sequence Label: pHCKan-yibDp-mdlC
FEATURES             Location/Qualifiers
     source          1..3605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..792)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(793..884)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(932..1670)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(1734..1751)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
       61 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
      121 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
      181 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
      241 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
      301 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
      361 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
      421 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
      481 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
      541 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
      601 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
      661 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
      721 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
      781 cgcttccctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagcta gctcactcgg
      901 tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac
      961 agattccgtg gataagcagg ggactaacat gtgaggcaaa acagcagggc cgcgccggtg
     1021 gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg cttttccggt
     1081 gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag
     1141 gacttaaaga tccccaccgt ttccggcggg tcgctccctc ttgcgctctc ctgttccgac
     1201 cctgccgttt accggatacc tgttccgcct ttctccctta cgggaagtgt ggcgctttct
     1261 catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta
     1321 agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt
     1381 ccaacccgga aaagcacggt aaaacgccac tggcagcagc cattggtaac tgggagttcg
     1441 cagaggattt gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta
     1501 cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag
     1561 ttccccaact gacttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag
     1621 ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctactg
     1681 aaccgctctg cgaaaaaacc ccgccgaagc ggggtttttt gcggtcaaca tcagtggtgg
     1741 tggtgatgat gcttattctg ctccgcgaac aattttccca tttttttcaa taacttgggg
     1801 gcatcctctt tttccaaaac cagttcaatc caatacatac ggttaacgtc cgcttgagct
     1861 tccttcataa cggatacgaa ctcattctcc gtgcgaacga tcttgcttac aacacggtcc
     1921 tctgttgctc caaaagtttc cggaagcttc gaataattcc acatgggaat gtcgttgtag
     1981 ctttgtgttg gcccatggat ctcgcgctcc acggtataac cgtcattatt aatgataaaa
     2041 cagattggat tcaatttttc gcggatagac aggcctaact cctgaacagt cagttgaagg
     2101 gacccgtcac caatgaataa cagatggcga ctctccttgt cggcaatctg agacccaaga
     2161 gcagcgggga acgtgtaccc gattgacccc cacagtggtt ggccgatgaa acgcgagttg
     2221 cttttaagga agatagtaga ggcaccaaaa aaagacgttc cttgttcagc gacaatagtt
     2281 tcatttgact gagtcaggct ctccactgct tgccataagc gatcttgaga aagtggtgcg
     2341 ctgctcggga taaactcttc gtactgttta tcgatatatt gtccttcgta ctcaatacct
     2401 ttaagttctg ataatgacga gacaactgcg cgaaaatcga aatcctctac caccttattg
     2461 aaaataatac cctcatcgat attaagagaa atcattttat tttcatccaa atgatgggta
     2521 aaagcgccag tagatgagtc cgttaatttc acgccaagca ttaagataaa atcggctgat
     2581 tcgacaaagt ttttaagaga gatctcactc agtttaccat tatagatccc caaaaacgaa
     2641 ggtaatgatt catcgaccgc agacttgccg aaattcagcg ttgtgattgg aagtttggtc
     2701 tcggagacga attgcgtgac tgttttctca agcccgaaac tgataacctc atgaccggca
     2761 ataacgactg gtttttgggc gttcttcaag gactcttcaa tctttgaaag aataacttgc
     2821 tcggtggtgt ttgtggttga actttccttc tccaaactca aggctggttt ctctgcctta
     2881 gctgccgcga catcgacggg taagttaatg taaactggtt tacgttcctt cagcagttga
     2941 cttaagacac gatcaatttc ataagtcgca ttctcggcgg tcaaaagagt gcgggcagct
     3001 gtcaccggtt catgcatctt catgaagtgc ttaaaatcgc catctgctaa agtgtggtgg
     3061 acgaatttac cgtcgttctg gaccttggag gttgggctac ctacgatttc tacaacgggc
     3121 agattttcag catacgagcc ggccaatcca ttgatagcac ttaactcccc cacgccgaaa
     3181 gttgtaagaa aagccgcggc tttttttgtg cgagcatatc catccgccat atatgatgcg
     3241 ttaagttcat tggcgttccc aatccatttc atatcctcac gtgaaatgat ctgatcaagg
     3301 aactgcaggt tatagtcccc gggtacacca aaaatttcct cgatgcctaa ctcgtgcagg
     3361 cgatcaagca agtagtcccc caccgtatac atagattatc ctcctacaca cgaaagttaa
     3421 acaaaattat ttgtagaggg aaaccgttgt ggtctccctg aatatagaag ttatcctgac
     3481 gttttacagg ctttgtatta agaaactatt aagcctgtga gatattattt ttacaggtga
     3541 gagagtcact tcagggtaga gagataatga gagcagctat ataagagatc agcacaatta
     3601 cgcac
//
