LOCUS       sequence-27313-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: GST-PIWI(aa478-860)
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    31..55
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      586..595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaatcggatc tggttccgcg tggatcatca acaagtttgt acaaaaaagc aggctcttta
       61 aaggaaccaa ttcagtcgac tggatcgatc tcgagagacg ctggcatgcc catccagggc
      121 cagccgtgct tctgcaaata cgcgcagggg gcggacagcg tggagcccat gttccggcac
      181 ctgaagaaca cgtatgcggg cctgcagctg gtggtggtca tcctgcccgg caagacgccc
      241 gtgtacgccg aggtcaagcg cgtgggagac acggtgctgg ggatggccac gcagtgcgtg
      301 cagatgaaga acgtgcagag gaccacgcca cagaccctgt ccaacctctg cctgaagatc
      361 aacgtcaagc tgggaggcgt gaacaacatc ctgctgcccc agggcaggcc gccggtgttc
      421 cagcagcccg tcatctttct gggagcagac gtcactcacc cccccgccgg ggatgggaag
      481 aagccctcca ttgccgccgt ggtgggcagc atggacgccc accccaatcg ctactgcgcc
      541 accgtgcgcg tgcagcagca ccggcaggag atcatacaag acctggccgc catggtccgc
      601 gagctcctca tccagttcta caagtccacg cgcttcaagc ccacccgcat catcttctac
      661 cgcgacggtg tctctgaagg ccagttccag caggttctcc accacgagtt gctggccatc
      721 cgtgaggcct gtatcaagct agaaaaagac taccagcccg ggatcac
//
