LOCUS       sequence-273135-       12928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  enSERT LacZ reporter vector for site-specific integration into the 
            H11 locus (contains Hsp68 minimal promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   Kvon EZ, Zhu Y, Kelman G, Novak CS, Plajzer-Frick I, Kato M, Garvin 
            TH, Pham Q, Harrington AN, Hunter RD, Godoy J, Meky EM, Akiyama JA, 
            Afzal V, Tran S, Escande F, Gilbert-Dussardier B, Jean-Marcais N, 
            Hudaiberdiev S, Ovcharenko I, Dobbs MB, Gurnett CA, Manouvrier-Hanu 
            S, Petit F, Visel A, Dickel DE, Pennacchio LA
  TITLE     Comprehensive In Vivo Interrogation Reveals Phenotypic Impact of 
            Human Enhancer Variants.
  JOURNAL   Cell. 2020 Mar 6. pii: S0092-8674(20)30208-7. doi: 
            10.1016/j.cell.2020.02.031.
  PUBMED    32169219
REFERENCE   2  (bases 1 to 12928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Mar 6. pii: S0092-8674(20)30208-7. doi: 10.1016/j.cell.2020.02.031."
COMMENT     Sequence Label: PCR4-Hsp68::lacZ-H11
FEATURES             Location/Qualifiers
     source          1..12928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(29..47)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(68..84)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(68..84)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(81..103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    92..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(116..122,123..140,141..146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    161..182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(299..316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(470..1058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(550..569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1182..1250,1251..2042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1400..1419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2292..3086)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2292..3086)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2403..2422)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3013..3032
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(3435..3737)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3537..3556)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     3874..3891
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3875..3891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3898..3917
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3898..3916
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7233..10280
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     polyA_signal    10428..10562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10465..10484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10519..10538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10559..10575)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10559..10575)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gaattcgttt aaacctgcag gactagtccc tttagtgagg gttaattctg agcttggcgt
       61 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      121 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      181 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagacaat
     1141 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1201 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1261 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1321 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1381 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1441 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1501 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1561 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1621 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1681 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1741 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1801 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1861 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1921 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1981 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2041 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2101 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gagcggatac atatttgaat
     2161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2221 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     2281 gcgttaataa ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     2341 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     2401 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     2461 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     2521 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     2581 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     2641 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     2701 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     2761 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     2821 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     2881 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     2941 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     3001 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     3061 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagag
     3121 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     3181 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     3241 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     3301 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     3361 cgtttctgcg gactggcttt ctacgtgaaa aggatctagg tgaagatcct ttttgataat
     3421 ctcatgcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
     3481 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
     3541 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
     3601 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgccccggc
     3661 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
     3721 gtgtaaacct taaactgccg tacgtatagg ctgcgcaact gttgggaagg gcgatcggtg
     3781 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3841 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     3901 tacgactcac tatagggcga attgaatttg ccctggtaca tgtatggtgg caagtgttca
     3961 ccacatccac ctccttgtaa gtatccaaat caacaaaagt agtaaacttt gacattttta
     4021 ggaacatcta caactcatga gtttcttctt tctccccatc aggtgtgctt cctcctggga
     4081 tagacatggc tcctttacag gggctatctg gcccactcct gggtcaaccc ttgtaccctt
     4141 tggtctctgc tgctagtcac cctctcctaa accctcgtcc tgggacccct ctgcatttgg
     4201 cggtgatgca gcagcagcta cagcgctcag gtaaggttta gaaataggcc aggttagctg
     4261 atcacactgg aaagttctga gatttccagg agcaatagtt atcactgccc ctttgtttct
     4321 taggtgtcac tagaaaaaca ggtgaagtga actgacctgt gcagtctcag ggattattac
     4381 tactgcaagg acagtcggag ccagccatgc tctagctctg tttttcactt cttataagcc
     4441 tgagaatttt tgctgagatg tgaacatgtc agccttggtg ggctttctta cgttgcattt
     4501 tcttacgttg cattttgcac tttaggaagc ctttggttaa atcctctggt agacttccca
     4561 tcccccaaag taacaaacca ttcaagcaaa gtaggtcaga aaatattgtc aatactgaga
     4621 acaggacttg ggagtgagta tccaagggtg gtcttagtag acttgtgggg ttctgtgggt
     4681 gaggtgtaga gtagagctac tttgctgcac tgacactctt ctctccatag ttctgcatcc
     4741 tccaggctct agttcccagg cagcagctat cagcgttcag actcctcaga atgtacccag
     4801 ccggtcgggc atgccccaca tgcactccca gctggagcat cgtaccagcc aaaggagcag
     4861 ctcccctgtg ggccttgcca aatggtttgg ctcagatgtg ctacagcagc ctctgccctc
     4921 catgcccacc aaagtcatca gtgtagatga actggaatat agacagtgaa gagggcaggc
     4981 tggctcaccc atacctggac ctgtggtgac accctggtca tgactctcat tccctctttg
     5041 taatgggctt ttacattgga gcacactatg tgaagatgtt taggggatcc acatacctac
     5101 catgatctac attatgacag aaggctgtta aatcgaatga acctacatgg ttcaaataca
     5161 agggatacaa gattgtcagt cctggaagtc tttcttttat aaaatatgtg aatgaagtgt
     5221 tggtgtcttc tagaggtgac acctaagggt tctgaaaaaa taaaatgtat agacccttat
     5281 gtacagacct gtgtataaac ttttgtacat acaaataggg tagctttttt tgaacttata
     5341 catacagctg tacataaagt aactatcagt taggcttgtg tcaactgttt ggattttttt
     5401 cacttgaata tttgggactt tttcttttgg tttattaaaa gttacatatg ccacgtgtgt
     5461 gaacgatatg gctggtactg tgtttatttc ttccatgaac taagacagtc taaatgagtt
     5521 cctttcacgt tttaatttta ccttaggact tctggaattt cttctgcaca taaagttctg
     5581 atagcattag tttaagctgg actaaccctg aaagtagctt gtggcaagta tcaaggaatc
     5641 aatattatac tctacaaaat caaagtttac agagaagtca tatagtaatt tttctgaaat
     5701 ttactggcac aatgttaatc cagcctgact ccaactaatt aatggtcaca ttaatttaag
     5761 tctttccctt gcctctgctg cattagtttc tctcaaaatt gttaacttac aacttgaagt
     5821 ctggtattat aaattgaatg taaagcattc tgaaagatac tatactgatt gcaggttttt
     5881 cagtcaggtt caagctaatt tgaccagtca ttggattaat tatggatctg gggccataaa
     5941 tgctatttta attccactat agagattaaa ataagccatt ctccatttca taatattcta
     6001 ttggactttg actgcagggg cctccaagtc ttgacagtag attataatcc ttcagctgcc
     6061 cactctactg gaggaggaca aactggtcac ttttcagcaa aacctggctg tggatcaggg
     6121 cagtctggta cttccaagct cattagatgc catcatgctc tcactgcctc ctcagcttca
     6181 agaggaatct ggaaaaagca gtcccactgg tcaggaaagg aacactagtg cacttatcct
     6241 gggtgtctgc tgagccaaca gtggtagtaa ggtaagggca ggatgtgtca aactgccaat
     6301 agagaactac ttactcttca ggctgaagct gatggaacag cggccgccag gaacatccaa
     6361 actgagcagc cggggtcccc cccacccccc accccgcccc tcccggcaac tttgagcctg
     6421 tgctgggaca gagcctctag ttcctaaatt agtccatgag gtcagaggca gcactgccat
     6481 tgtaacgcga ttggagagga tcacgtcacc ggacacgccc ccaggcatct ccctgggtct
     6541 cctaaacttg gcggggagaa gttttagccc ttaagtttta gcctttaacc cccatattca
     6601 gaactgtgcg agttggcgaa accccacaaa tcacaacaaa ctgtacacaa caccgagcta
     6661 gaggtgatct ttcttgtcca ttccacacag gccttagtaa tgcgtcgcca tagcaacagt
     6721 gtcactagta gcaccagcac ttccccacac cctccccctc aggaatccgt actctccagt
     6781 gaaccccaga aacctctgga gagttctgga caagggcgga acccacaact ccgattactc
     6841 aagggaggcg gggaagctcc accagacgcg aaactgctgg aagattcctg gccccaaggc
     6901 ctcctccggc tcgctgattg gcccagcgga gagtgggcgg ggccggtgaa gactccttaa
     6961 aggcgcaggg cggcgagcag gtcaccagac gctgacagct actcagaacc aaatctggtt
     7021 ccatccagag acaagcgaag acaagagaag cagagcgagc ggcgcgttcc cgatcctcgg
     7081 ccaggaccag ccttccccag agcatccctg ccgcggagcg caaccttccc aggagcatcc
     7141 ctgccgcgga gcgcaacttt ccccggagca tccacgccgc ggagcgcagc cttccagaag
     7201 cagagcgcgg cgccatggct cgcgatgatc ccgtcgtttt acaacgtcgt gactgggaaa
     7261 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7321 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7381 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
     7441 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
     7501 ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
     7561 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
     7621 agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct
     7681 gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac
     7741 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg
     7801 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac
     7861 cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg
     7921 ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag
     7981 tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa
     8041 ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc
     8101 cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg
     8161 ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg
     8221 aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg
     8281 agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc
     8341 tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt
     8401 ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc
     8461 acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg
     8521 aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc
     8581 tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg
     8641 tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata
     8701 ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat
     8761 ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat
     8821 acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc
     8881 agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat
     8941 atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg
     9001 atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga
     9061 cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag
     9121 tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc
     9181 tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac
     9241 agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag
     9301 tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc
     9361 agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc
     9421 cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat
     9481 ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga
     9541 cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag
     9601 cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg
     9661 ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga
     9721 ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga
     9781 ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc
     9841 atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc
     9901 tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct
     9961 gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc
    10021 gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca
    10081 acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg
    10141 cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact
    10201 cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt
    10261 tggtctggtg tcaaaaataa taataaccgg gcagggggga tctaagctct agataagtaa
    10321 tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac
    10381 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    10441 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    10501 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    10561 tccactagtt ctagagcggc aagcagaaag ggaacaaggg gatattaaat gtgtatagac
    10621 acgctagaga gatggctcag cagttaagag aactagctgg tctttcagag gtcctgagat
    10681 caattttaga cacccacatg gtggctcatg accatctatc tataaatgga tctgattttc
    10741 atgtctggca gtgtacagaa gctaactgaa gaaaggtgga agacccacaa gagttcaaga
    10801 taagccctat atagtgaagt tcaaggcaag ctttttctac ctgaaactta gtctcaaaaa
    10861 aaaatgaata cgtaaacagt cttccagggg ataagaacct tacagaaaaa gcagaaatgc
    10921 ctggggcact ggattaccga tgtaatcaaa ttcagtcctt gaattgaaca caggattgcc
    10981 tagagcaagg ccagccagag attcatctca gagggagaaa ggtgtctttg gagcaatttt
    11041 gtggtaatct agtatgtatc acataagttt agacgcattt gggactggaa agatgtgaac
    11101 aaagcaccct atggctcaca tctgtcatta actctagttc cagtgcacct gacaccgtct
    11161 tctggcctct gcagtgacca agcacatggg tagtatgtag acatatacat aagcaaaaca
    11221 cacatcatta aaaagtgaca tttcccaaag gaagctgaag aaccagttct tgagaagata
    11281 gtagaaatca gaaggggaaa tagtagacat acagagggac tgaccaggtt gtgtcacctt
    11341 tataggctag gctaattgat gatcgacact agcgctcttt gtgaaggaca cacaaatgag
    11401 acatagttta taggactaaa cacacttcta agcaatttaa tgagacttaa gaccctgtct
    11461 ctagcaaata ctctggatga tattcagctc aaggctcttg tcagacatgt ttccattttc
    11521 aaggtgagct aactggccaa aactgccaac aacctgtagt gaatagagaa gatgagaaaa
    11581 tctggattct caaatgacct aatgaaagcc actggagcgc catatggttt ctgtgaaaat
    11641 gccttttcaa tcattaacct cttaaatgag tgttagcatc ctaactaatg agtggtgcag
    11701 aatagtgggt ctgcttagct taactaaggc caagaaaaca aaacaggaaa ttcattccat
    11761 gtcatgagac tcatactacg aggttccctt agacctcagg agaaaaagtc tttggctgta
    11821 agaacacacc tcagtggatg tggtagacta tgcctttact cttttttttt tttttttttt
    11881 tgggtttttt cgagacaggg tttctctgtg tagccttggc tgtcctggaa ctcactttgt
    11941 tgaccaggct ggcctcgaac tcagagatcc gcctgcctct gcctctcaag tgctagaatt
    12001 aaaagtgtgc gccaccactc ccagctatat agactatgcc tttaatcagg acacttggga
    12061 ggcaggggga tctcaaattc aattccagac agatgactga caaacacaca catacaccat
    12121 tcgtcttttc tttttttctt ttttcttttg aaaacaggtt tctctatgta gccctggctt
    12181 tcctagaatt caatctatag acccagctgg cctcaaacac agaacctctt gcctctgcct
    12241 cctcaacact atgactaaag gtgtgaacca ccaccaccac cacccacctg gccaaagaat
    12301 gaattgagtg aataagtacg agtgcaactc tccagcagcc tgaggcaaga aagcttaaca
    12361 gttgaacctg agaccctact tcgtttgtgt tgtacatatc ttctcataat gaactaggca
    12421 gcctagtctc tctaccaaat acaccatgca cttacacctc ctcaaaccaa ctgcaacagc
    12481 tacacacatt tccatggctt gcctgtaata ataaattcac aaacttcctc taagtaactt
    12541 ttagtggaaa tctaccaggt tatgcctatt tgtgattggg tgcacagatg actagatgag
    12601 atgtaatttg ggaagtgaca tgcaatagga aaaatgaata tataacctat aaatagaaaa
    12661 caccaaggca agcgtggtga ctcacacctt taattagcac ttgggaggca gaagtcgcat
    12721 catctcttag gtcaaggcca atctagttcc agaccagcaa ggacaacaga aaccttgcct
    12781 tgatataaac attttaaaac tttaaaaata gagaagccca aactctgctc attggtaatc
    12841 tacccttcct ttctttagac accttgagaa gctaggctac atagctttgg gtccttacgt
    12901 acccccactc actcagcctg cagtcaat
//
