LOCUS       sequence-273136-       12607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  enSERT LacZ reporter vector for site-specific integration into the 
            H11 locus (contains Shh minimal promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Kvon EZ, Zhu Y, Kelman G, Novak CS, Plajzer-Frick I, Kato M, Garvin 
            TH, Pham Q, Harrington AN, Hunter RD, Godoy J, Meky EM, Akiyama JA, 
            Afzal V, Tran S, Escande F, Gilbert-Dussardier B, Jean-Marcais N, 
            Hudaiberdiev S, Ovcharenko I, Dobbs MB, Gurnett CA, Manouvrier-Hanu 
            S, Petit F, Visel A, Dickel DE, Pennacchio LA
  TITLE     Comprehensive In Vivo Interrogation Reveals Phenotypic Impact of 
            Human Enhancer Variants.
  JOURNAL   Cell. 2020 Mar 6. pii: S0092-8674(20)30208-7. doi: 
            10.1016/j.cell.2020.02.031.
  PUBMED    32169219
REFERENCE   2  (bases 1 to 12607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Mar 6. pii: S0092-8674(20)30208-7. doi: 10.1016/j.cell.2020.02.031."
COMMENT     Sequence Label: PCR4-Shh::lacZ-H11
FEATURES             Location/Qualifiers
     source          1..12607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(29..47)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(68..84)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(68..84)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(81..103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    92..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(116..122,123..140,141..146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    161..182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(299..316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(470..1058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(550..569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1182..1250,1251..2042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1400..1419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2292..3086)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2292..3086)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2403..2422)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3013..3032
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(3435..3737)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3537..3556)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     3874..3891
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3875..3891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3898..3917
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3898..3916
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6912..9959
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     polyA_signal    10107..10241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10144..10163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10198..10217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10238..10254)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10238..10254)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gaattcgttt aaacctgcag gactagtccc tttagtgagg gttaattctg agcttggcgt
       61 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      121 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      181 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagacaat
     1141 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1201 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1261 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1321 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1381 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1441 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1501 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1561 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1621 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1681 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1741 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1801 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1861 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1921 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1981 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2041 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2101 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gagcggatac atatttgaat
     2161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2221 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     2281 gcgttaataa ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     2341 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     2401 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     2461 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     2521 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     2581 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     2641 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     2701 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     2761 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     2821 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     2881 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     2941 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     3001 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     3061 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagag
     3121 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     3181 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     3241 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     3301 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     3361 cgtttctgcg gactggcttt ctacgtgaaa aggatctagg tgaagatcct ttttgataat
     3421 ctcatgcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
     3481 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
     3541 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
     3601 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgccccggc
     3661 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
     3721 gtgtaaacct taaactgccg tacgtatagg ctgcgcaact gttgggaagg gcgatcggtg
     3781 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3841 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     3901 tacgactcac tatagggcga attgaattta gccctggtac atgtatggtg gcaagtgttc
     3961 accacatcca cctccttgta agtatccaaa tcaacaaaag tagtaaactt tgacattttt
     4021 aggaacatct acaactcatg agtttcttct ttctccccat caggtgtgct tcctcctggg
     4081 atagacatgg ctcctttaca ggggctatct ggcccactcc tgggtcaacc cttgtaccct
     4141 ttggtctctg ctgctagtca ccctctccta aaccctcgtc ctgggacccc tctgcatttg
     4201 gcggtgatgc agcagcagct acagcgctca ggtaaggttt agaaataggc caggttagct
     4261 gatcacactg gaaagttctg agatttccag gagcaatagt tatcactgcc cctttgtttc
     4321 ttaggtgtca ctagaaaaac aggtgaagtg aactgacctg tgcagtctca gggattatta
     4381 ctactgcaag gacagtcgga gccagccatg ctctagctct gtttttcact tcttataagc
     4441 ctgagaattt ttgctgagat gtgaacatgt cagccttggt gggctttctt acgttgcatt
     4501 ttcttacgtt gcattttgca ctttaggaag cctttggtta aatcctctgg tagacttccc
     4561 atcccccaaa gtaacaaacc attcaagcaa agtaggtcag aaaatattgt caatactgag
     4621 aacaggactt gggagtgagt atccaagggt ggtcttagta gacttgtggg gttctgtggg
     4681 tgaggtgtag agtagagcta ctttgctgca ctgacactct tctctccata gttctgcatc
     4741 ctccaggctc tagttcccag gcagcagcta tcagcgttca gactcctcag aatgtaccca
     4801 gccggtcggg catgccccac atgcactccc agctggagca tcgtaccagc caaaggagca
     4861 gctcccctgt gggccttgcc aaatggtttg gctcagatgt gctacagcag cctctgccct
     4921 ccatgcccac caaagtcatc agtgtagatg aactggaata tagacagtga agagggcagg
     4981 ctggctcacc catacctgga cctgtggtga caccctggtc atgactctca ttccctcttt
     5041 gtaatgggct tttacattgg agcacactat gtgaagatgt ttaggggatc cacataccta
     5101 ccatgatcta cattatgaca gaaggctgtt aaatcgaatg aacctacatg gttcaaatac
     5161 aagggataca agattgtcag tcctggaagt ctttctttta taaaatatgt gaatgaagtg
     5221 ttggtgtctt ctagaggtga cacctaaggg ttctgaaaaa ataaaatgta tagaccctta
     5281 tgtacagacc tgtgtataaa cttttgtaca tacaaatagg gtagcttttt ttgaacttat
     5341 acatacagct gtacataaag taactatcag ttaggcttgt gtcaactgtt tggatttttt
     5401 tcacttgaat atttgggact ttttcttttg gtttattaaa agttacatat gccacgtgtg
     5461 tgaacgatat ggctggtact gtgtttattt cttccatgaa ctaagacagt ctaaatgagt
     5521 tcctttcacg ttttaatttt accttaggac ttctggaatt tcttctgcac ataaagttct
     5581 gatagcatta gtttaagctg gactaaccct gaaagtagct tgtggcaagt atcaaggaat
     5641 caatattata ctctacaaaa tcaaagttta cagagaagtc atatagtaat ttttctgaaa
     5701 tttactggca caatgttaat ccagcctgac tccaactaat taatggtcac attaatttaa
     5761 gtctttccct tgcctctgct gcattagttt ctctcaaaat tgttaactta caacttgaag
     5821 tctggtatta taaattgaat gtaaagcatt ctgaaagata ctatactgat tgcaggtttt
     5881 tcagtcaggt tcaagctaat ttgaccagtc attggattaa ttatggatct ggggccataa
     5941 atgctatttt aattccacta tagagattaa aataagccat tctccatttc ataatattct
     6001 attggacttt gactgcaggg gcctccaagt cttgacagta gattataatc cttcagctgc
     6061 ccactctact ggaggaggac aaactggtca cttttcagca aaacctggct gtggatcagg
     6121 gcagtctggt acttccaagc tcattagatg ccatcatgct ctcactgcct cctcagcttc
     6181 aagaggaatc tggaaaaagc agtcccactg gtcaggaaag gaacactagt gcacttatcc
     6241 tgggtgtctg ctgagccaac agtggtagta aggtaagggc aggatgtgtc aaactgccaa
     6301 tagagaacta cttactcttc aggctgaagc tgatggaaca gcggccgcca ggaacatcca
     6361 aactgagcag cctttctttc tctctttctt tcttttttaa actggaagcc ttcaggtgaa
     6421 aataaagcca cagcagccag aggggggaga aatgtagtct ttctcagggt taacatcaga
     6481 agacaagctt gtgcggcttg gccaatcaga tgcgcccctg ccagctataa tatagtgcga
     6541 aaggcagcct gtctcacaag ctctccagcc ttgctaccat ttaaaatcag gctctttttg
     6601 tcttttaatt gctgtctcga gacccaactc cgatgtgttc cgttaccagc gaccggcagc
     6661 ctgccatcgc agccccagtc tgggtgggga tcggagacaa gtcccctgca gcagcggcag
     6721 gcaaggttat ataggaagag aaagagccag gcagcgccag agggaacgaa cgagccgagc
     6781 gaggaaggga gagccgagcg caaggaggag cgcacacgca cacacccgcg cgtacccgct
     6841 cgcgcacaga cagcgcgggg acagctcaca agtcctcagg ttccgcggac gagatggctc
     6901 gcgatgatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6961 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7021 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg
     7081 caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg
     7141 tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtgacct
     7201 atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc
     7261 tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg
     7321 gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca
     7381 gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg
     7441 cggtgatggt gctgcgctgg agtgacggca gttatctgga agatcaggat atgtggcgga
     7501 tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt
     7561 tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc
     7621 agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa
     7681 cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt
     7741 atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa
     7801 tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag
     7861 cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga
     7921 acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc
     7981 aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta
     8041 acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct
     8101 acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc
     8161 gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc
     8221 agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg
     8281 gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc
     8341 agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc
     8401 gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt
     8461 cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca
     8521 gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg
     8581 gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt
     8641 ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg
     8701 gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc
     8761 agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc
     8821 gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa
     8881 gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac
     8941 taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg
     9001 cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa
     9061 acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa
     9121 tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc
     9181 tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca
     9241 cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg
     9301 cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt
     9361 gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc
     9421 aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg
     9481 cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga
     9541 actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa
     9601 actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca
     9661 tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga
     9721 attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac
     9781 agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga
     9841 atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg
     9901 cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataat
     9961 aataaccggg caggggggat ctaagctcta gataagtaat gatcataatc agccatatca
    10021 catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    10081 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    10141 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10201 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccactagttc tagagcggca
    10261 agcagaaagg gaacaagggg atattaaatg tgtatagaca cgctagagag atggctcagc
    10321 agttaagaga actagctggt ctttcagagg tcccgagatc aattttagac acccacatgg
    10381 tggctcatga ccatctatct ataaatggat ctgattttca tgtctggcag tgtacagaag
    10441 ctaactgaag aaaggtggaa gacccacaag agttcaagat aagccctata tagtgaagtt
    10501 caaggcaagc tttttctacc tgaaacttag tctcaaaaaa aaatgaatac gtaaacagtc
    10561 ttccagggga taagaacctt acagaaaaag cagaaatgcc tggggcactg gattaccgat
    10621 gtaatcaaat tcagtccttg aattgaacac aggattgcct agagcaaggc cagccagaga
    10681 ttcatctcag agggagaaag gtgtctttgg agcaattttg tggtaatcta gtatgtatca
    10741 cataagttta gacgcatttg ggactggaaa gatgtgaaca aagcacccta tggctcacat
    10801 ctgtcattaa ctctagttcc agtgcacctg acaccgtctt ctggcctctg cagtgaccaa
    10861 gcacatgggt agtatgtaga catatacata agcaaaacac acatcattaa aaagtgacat
    10921 ttcccaaagg aagctgaaga accagttctt gagaagatag tagaaatcag aaggggaaat
    10981 agtagacata cagagggact gaccaggttg tgtcaccttt ataggctagg ctaattgatg
    11041 atcgacacta gcgctctttg tgaaggacac acaaatgaga catagtttat aggactaaac
    11101 acacttctaa gcaatttaat gagacttaag accctgtctc tagcaaatac tctggatgat
    11161 attcagctca aggctcttgt cagacatgtt tccattttca aggtgagcta actggccaaa
    11221 actgccaaca acctgtagtg aatagagaag atgagaaaat ctggattctc aaatgaccta
    11281 atgaaagcca ctggagcgcc atatggtttc tgtgaaaatg ccttttcaat cattaacctc
    11341 ttaaatgagt gttagcatcc taactaatga gtggtgcaga atagtgggtc tgcttagctt
    11401 aactaaggcc aagaaaacaa aacaggaaat tcattccatg tcatgagact catactacga
    11461 ggttccctta gacctcagga gaaaaagtct ttggctgtaa gaacacacct cagtggatgt
    11521 ggtagactat gcctttactc tttttttttt tttttttttt gggttttttc gagacagggt
    11581 ttctctgtgt agccttggct gtcctggaac tcactttgtt gaccaggctg gcctcgaact
    11641 cagagatccg cctgcctctg cctctcaagt gctagaatta aaagtgtgcg ccaccactcc
    11701 cagctatata gactatgcct ttaatcagga cacttgggag gcagggggat ctcaaattca
    11761 attccagaca gatgactgac aaacacacac atacaccatt cgtcttttct ttttttcttt
    11821 tttcttttga aaacaggttt ctctatgtag ccctggcttt cctagaattc aatctataga
    11881 cccagctggc ctcaaacaca gaacctcttg cctctgcctc ctcaacacta tgactaaagg
    11941 tgtgaaccac caccaccacc acccacctgg ccaaagaatg aattgagtga ataagtacga
    12001 gtgcaactct ccagcagcct gaggcaagaa agcttaacag ttgaacctga gaccctactt
    12061 cgtttgtgtt gtacatatct tctcataatg aactaggcag cctagtctct ctaccaaata
    12121 caccatgcac ttacacctcc tcaaaccaac tgcaacagct acacacattt ccatggcttg
    12181 cctgtaataa taaattcaca aacttcctct aagtaacttt tagtggaaat ctaccaggtt
    12241 atgcctattt gtgattgggt gcacagatga ctagatgaga tgtaatttgg gaagtgacat
    12301 gcaataggaa aaatgaatat ataacctata aatagaaaac accaaggcaa gcgtggtgac
    12361 tcacaccttt aattagcact tgggaggcag aagtcgcatc atctcttagg tcaaggccaa
    12421 tctagttcca gaccagcaag gacaacagaa accttgcctt gatataaaca ttttaaaact
    12481 ttaaaaatag agaagcccaa actctgctca ttggtaatct acccttcctt tctttagaca
    12541 ccttgagaag ctaggctaca tagctttggg tccttacgta cccccactca ctcagcctgc
    12601 agtcaat
//
