LOCUS       sequence-273176-        3513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr371.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr371
FEATURES             Location/Qualifiers
     source          1..3513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    124..145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(160..165,166..183,184..190)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    198..214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     203..225
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     222..238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     222..238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        256..274
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     256..273
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      866..875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      870..879
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(909..928)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(910..928)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(935..952)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(935..951)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(944..966)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1092..1547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1179..1198)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1389..1410
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1625..1729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1730..1798,1799..2590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1948..1967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2761..3349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3250..3269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3503..3513,1..7)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
       61 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      121 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      181 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      241 tgattacgcc aagctattta ggtgacacta tagaatactc aagctatgca tccaacgcgt
      301 tgggagctct cccatatggt cgacctgcag gcggccgcga attcactagt gattactgtc
      361 ccaaagatgc tgctgggcat ccagactcag agtaagctca tcacctatgc aggctgcatc
      421 acacagatgt acttcttcac agtcttcgga cttctggaca atctgcttct gactgtgatg
      481 gcatatgacc gctttgtggc catctgccac cccttgcact atacagtgct catgaaccct
      541 aagctctgtt cccagctact tctcctggca tggcttataa gcatacttgg agccctgcct
      601 gagagtttaa ctgcactgag actgtctttc tgtgcagtcg tggaaattcc acactatttt
      661 tgtgaactcc ctgaagtcct taagctagcc tgctctgaca ccttcatcaa taacgttgtg
      721 ttatatattg taacaggcat catgggcttt tttcctcttg ctgggatact tttctcttac
      781 tctcaaattg tgacatctgt cttgcggatt tcaacagtgg gaggaaagta taaagcgttt
      841 tccacctgtg aatcgaattc ccgcggccgc catggcggcc gggagcatgc gacgtcgggc
      901 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
      961 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1021 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1081 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1141 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1201 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1261 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1321 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1381 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1441 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1501 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat
     1561 tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa
     1621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1681 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1741 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1801 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1861 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1921 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1981 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2041 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2101 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2161 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2221 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2281 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2341 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2401 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2461 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2521 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2581 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2641 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2701 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3421 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3481 ccgcagccga acgaccgagc gcagcgagtc agt
//
