LOCUS       sequence-273177-        3473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr374.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr374
FEATURES             Location/Qualifiers
     source          1..3473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     114..133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     367..384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    501..522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(537..542,543..560,561..567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    575..591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     580..602
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     599..615
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     599..615
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        633..651
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     633..650
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1203..1212
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1207..1216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1246..1265)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1247..1265)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1272..1289)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1272..1288)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1281..1303)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1429..1884
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1516..1535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1726..1747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1962..2066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2067..2135,2136..2927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2285..2304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3098..3473,1..213)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
       61 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      241 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      301 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      361 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      421 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      481 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      541 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      601 ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag
      661 ctatgcatcc aacgcgttgg gagctctccc atatggtcga cctgcaggcg gccgcgaatt
      721 cactagtgat tttttctgcc ttcctgagca tgtaccttct cacaggccta gggaacctgc
      781 tcatcatcct ggccattgct gctgatccca ggctgcacac ccccatgtac tttttcctgg
      841 ccaacctggc ctttgtagat gtctgcttca cctccaccac catccccaag atgctggcca
      901 accatgtgtc aggacacaaa ggaatctctt actctggttg cctcacacag atgttctttt
      961 tcatctggtt tgccggcatt gacagcttct tgctgactgc catggcctat gaccgctttg
     1021 tggccatctg tcaccctcta cattatacaa cctcgatcac accacggctg tgtggcttcc
     1081 tggtcactgc atcctgggcc tcagcctttg caaatgccct gacccacaca gtccttctga
     1141 ctcgcctctt gttctgtggc cacaaccagg tcccccactt cttctgtaat cgaattcccg
     1201 cggccgccat ggcggccggg agcatgcgac gtcgggccca attcgcccta tagtgagtcg
     1261 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1321 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1381 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     1441 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1501 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1561 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1621 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1681 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     1741 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     1801 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     1861 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     1921 tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1981 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2041 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2101 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2161 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2221 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2281 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2341 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2401 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2461 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2521 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2581 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2641 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2701 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2761 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2821 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2881 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2941 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3001 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3061 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3121 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3181 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3241 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3301 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3361 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3421 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg aga
//
