LOCUS       sequence-273195-        4260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr713.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr713
FEATURES             Location/Qualifiers
     source          1..4260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     417..434
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    551..572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(587..592,593..610,611..617)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    625..641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     630..652
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     649..665
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     649..665
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        683..701
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     683..700
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2040..2049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2044..2053
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2083..2102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2084..2102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2109..2126)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2109..2125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2118..2140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2266..2721
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2353..2372)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2563..2584
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2799..2903
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2904..2972,2973..3764)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3122..3141)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3935..4260,1..263)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
       61 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      121 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      181 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      241 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      301 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      361 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      421 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      481 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      541 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      601 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      661 atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag ctatgcatcc
      721 aacgcgttgg gagctctccc atatggtcga cctgcaggcg gccgcgaatt cactagtgat
      781 tcgttgtcct acactgttgt gactcccatg ttgaacccca tcatctatag cctgagaaat
      841 aatgaggtga agagtgccct cagcaggacc ttccacaagg ccctggccct cagaaaccat
      901 atcacataaa catcaaatcc tctctcctcc ttaaatgtcc attggaatga caccaacttt
      961 ttaaatggat gttaggaagc tcaggagaga caaaaactca gataggttat tgatatcctt
     1021 ctatatttct ttgcctttcc attgtataaa cattgttttt tctcatcaga ataattagta
     1081 ttttggatgc ttctatgatt aatttgttca aggtattatt ttgctaatca gcatgaaagt
     1141 gacatagatt gtgcactgta tggaattcct aatgtgtact tggagacata catgagaaaa
     1201 tacagtaaaa accgtcaaaa gtcatcctgg ccttttgtct ggatttagac tagtttgaat
     1261 ccaggtgcaa agaaacatcc cattctttat tgcatgtttc ttttgttctt tcattttatt
     1321 tttatagttt gagacctcaa acagaagaag gcaaactact aatccacgtg tatatttata
     1381 gaacatcaat agcctcattg actttttact caggctcctt cggtcaggta gagcaagccc
     1441 agtctacagt tcttcatttt aaggctctaa acctttgttt ttagaagtta aaaaatttat
     1501 atgctatctt ccatgatact ctcctctgat ttccatggat ctttacagac tccagtacaa
     1561 gtccttctac gaatgctatt gtattgcact cagtgacttt gcatcccaaa gttacatgca
     1621 ctgtcagcat gaaaaggcaa aagggcacca caatagaata acttaatcaa tattgaggag
     1681 ttcaaatgga gttacttcta agaggaaatg gattttattg ctgaggaagt gaaaccaaag
     1741 atgcttaaac aaatataatt ggaatacaat tctaaacccc aagtttttga atattagttt
     1801 tgtacttttt cactgccata tatgaaaatt gtttagttca gagtcatggt aaaacaatat
     1861 tttataaggg tgggttgaga gagaagtaag tgggcaagat tatgtttgag aaaaacataa
     1921 tgagcatcca tttaatacac acttcaacaa aattagcaaa atagagctat atctataaga
     1981 attcaataca aatagcctca tactgtgcca tgttgatcag accaaatcga attcccgcgg
     2041 ccgccatggc ggccgggagc atgcgacgtc gggcccaatt cgccctatag tgagtcgtat
     2101 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     2161 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     2221 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
     2281 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2341 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2401 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2461 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2521 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2581 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2641 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2701 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2761 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2821 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2881 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2941 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3001 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3061 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     3121 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     3181 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     3241 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     3301 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     3361 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3421 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3481 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3541 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3601 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3661 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3721 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3781 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3841 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3901 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3961 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4021 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4081 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4141 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4201 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
//
