LOCUS       sequence-273213-        3487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr855.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr855
FEATURES             Location/Qualifiers
     source          1..3487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     155..174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     408..425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    542..563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(578..583,584..601,602..608)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    616..632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     621..643
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     640..656
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     640..656
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        674..692
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     674..691
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1258..1267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1262..1271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1301..1320)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1302..1320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1327..1344)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1327..1343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1336..1358)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1484..1939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1571..1590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1781..1802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2017..2121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2122..2190,2191..2982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2340..2359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3153..3487,1..254)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
       61 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      121 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      181 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      241 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      301 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      361 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      421 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      481 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      541 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      601 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      661 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg
      721 ggagctctcc catatggtcg acctgcaggc ggccgcgaat tcactagtga ttccttggga
      781 acctgctcat aattctgtgt ataagctctg agtcccacct ccaaaccccc atgtacttct
      841 ttctctctaa cttgtccttt aatgacattg gtttaagcac agccacagtc atcaagatgc
      901 tggttaacat ccaagcaaat gaccagagca tcacttacac agactgcctc acacagctct
      961 tctttgtact tgcttttgca tactttgaaa attttctcct tacagtgatg gcctatgatc
     1021 gctatgcagc tatttgtcac ccattaaggt ataatatcat tatgaatcct aacttatgtg
     1081 ttctactctc actaaattct ctgttcatta gtgttatgga ttccctgatt cacacactta
     1141 tggtgcagcg gctgtccttc tgcacagatc tggaaatccc tcacttcttc tgtgagcttg
     1201 atcaggtcat caagctttcc tgttctgaca cccgaattga caaatcgaat tcccgcggcc
     1261 gccatggcgg ccgggagcat gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta
     1321 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     1381 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     1441 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     1501 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     1561 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     1621 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     1681 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1741 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     1801 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1861 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1921 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     1981 cacaccgcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2101 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2161 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2221 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2281 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2341 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2401 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2461 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2521 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2581 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2641 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2701 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2761 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2821 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2881 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2941 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3001 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3061 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3481 tcgtgca
//
