LOCUS       sequence-273243-        9802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and 
            puromycin resistance for selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, 
            Lawson ND
  TITLE     Genomic Characterization of Endothelial Enhancers Reveals a 
            Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene 
            Expression.
  JOURNAL   Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.
  PUBMED    32065070
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075."
COMMENT     Sequence Label: pLenti-CMVSP6-NR2F2-SV40-PURO
FEATURES             Location/Qualifiers
     source          1..9802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2100
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2168..2188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2212..3196
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        3231..3249
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3231..3248
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    3249..3273
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3278..3287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4161..4178
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     protein_bind    complement(4530..4554)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4554..4883
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4734..4869
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4893..5492
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4893..4912)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5389..5409
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(5792..5812)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5827..6415
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5880..5900)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6298..6309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6502..6735
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6807..6928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6844..6863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6898..6917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6968..7103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7123..7142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7124..7142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7152..7169)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7152..7168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7161..7183)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7310..7765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7397..7416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7607..7628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7791..7895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7896..7964,7965..8756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8114..8133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8927..9515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9416..9435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9669..9686
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2101 atcgatcacg agactagcct cctagcccgg gctcgagcag ctgaagcttg catgcctgca
     2161 gggctatcaa ctttgtatag aaaagttgaa ggcctcttcg ctattacgcc agtcgaccgc
     2221 caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc
     2281 caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc
     2341 caattcaata tggcggactt ggcaccatgc caattcaata tggcggacct ggcactgtgc
     2401 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
     2461 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
     2521 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
     2581 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
     2641 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
     2701 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
     2761 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
     2821 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
     2881 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
     2941 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
     3001 ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
     3061 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
     3121 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
     3181 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
     3241 cactatagac aagtttgtac aaaaaagcag gctctccgcc accatggcaa tggtagtcag
     3301 cacgtggcgc gacccccagg acgaggtgcc cggctcacag ggcagccagg cctcgcaggc
     3361 gccgcccgtg cccggcccgc cgcccggcgc cccgcacacg ccacagacgc ccggccaagg
     3421 gggcccagcc agcacgccag cccagacggc ggccggtggc cagggcggcc ctggcggccc
     3481 gggtagcgac aagcagcagc agcagcaaca catcgagtgc gtggtgtgcg gagacaagtc
     3541 gagcggcaag cactacggcc agttcacgtg cgagggctgc aagagcttct tcaagcgcag
     3601 cgtgcggagg aacctgagct acacgtgccg cgccaaccgg aactgtccca tcgaccagca
     3661 ccatcgcaac cagtgccagt actgccgcct caaaaagtgc ctcaaagtgg gcatgagacg
     3721 ggaagcggtg cagaggggca ggatgccgcc gacccagccg acccacgggc agttcgcgct
     3781 gaccaacggg gatcccctca actgccactc gtacctgtcc ggatatattt ccctgctgtt
     3841 gcgcgcggag ccctatccca cgtcgcgctt cggcagccaa tgcatgcagc ccaacaacat
     3901 catgggtatc gagaacattt gcgaactggc cgcgaggatg ctcttcagcg ccgtcgagtg
     3961 ggcccggaac atccccttct tccccgacct gcagatcacg gaccaggtgg ccctgcttcg
     4021 cctcacctgg agcgagctgt ttgtgttgaa tgcggcgcag tgctccatgc ccctccacgt
     4081 cgccccgctc ctggccgccg ccggcctgca tgcttcgccc atgtccgccg accgggtggt
     4141 cgcctttatg gaccacatac ggatcttcca agagcaagtg gagaagctca aggcgctgca
     4201 cgttgactca gccgagtaca gctgcctcaa ggccatagtc ctgttcacct cagatgcctg
     4261 tggtctctct gatgtagccc atgtggaaag cttgcaggaa aagtctcagt gtgctttgga
     4321 agaatacgtt aggagccagt accccaacca gccgacgaga ttcggaaagc ttttgcttcg
     4381 cctcccttcc ctccgcaccg tctcctcctc agtcatagag caattgtttt tcgtccgttt
     4441 ggtaggtaaa acccccatcg aaaccctcat ccgggatatg ttactgtccg gcagcagttt
     4501 taactggccg tatatggcaa ttcaataaca cccagctttc ttgtacaaag tgggtgtgtc
     4561 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4621 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4681 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4801 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4861 ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc acggtgcgcc
     4921 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     4981 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     5041 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     5101 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     5161 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     5221 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     5281 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5341 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     5401 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     5461 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     5521 ccgaaaggag cgcacgaccc catgcatcga taaaataaaa gattttattt agtctccaga
     5581 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     5641 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     5701 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     5761 gctcagggcc aagaacagat ggaacagctg acaactttat tatacatagt tgataattca
     5821 ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5881 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5941 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6001 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6061 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6121 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6181 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6241 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6301 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6361 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     6421 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6481 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6541 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6721 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6781 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6901 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7081 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7141 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7201 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7261 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7381 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7501 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7621 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7681 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7741 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7801 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7861 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7921 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7981 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8041 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8101 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8161 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8221 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8281 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8341 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8401 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8461 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8521 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8581 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8641 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8701 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8761 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8821 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9061 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9721 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9781 cgggcagtga gcgcaacgca at
//
