LOCUS       sequence-273247-       10984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid contains 161k modified human enhancer with with 
            ETS binding sites mutated driving firefly Luciferase 2 and Renilla 
            Luciferase driven by TK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, 
            Lawson ND
  TITLE     Genomic Characterization of Endothelial Enhancers Reveals a 
            Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene 
            Expression.
  JOURNAL   Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.
  PUBMED    32065070
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075."
COMMENT     Sequence Label: pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK...
FEATURES             Location/Qualifiers
     source          1..10984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2100
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2168..2188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3179..3203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3210..3241
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3268..3277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3274..4926
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    complement(4940..4964)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4965..5716
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          5786..5918
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5901..5920
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5963..5981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5991..6926
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(6974..6994)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7009..7597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7062..7082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7480..7491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7684..7917
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7989..8110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8026..8045)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8080..8099
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8150..8285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8306..8324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8334..8351)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8334..8350)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8343..8365)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8492..8947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8579..8598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8789..8810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8973..9077
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9078..9146,9147..9938)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9296..9315)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10109..10697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10598..10617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10851..10868
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2101 atcgatcacg agactagcct cctagcccgg gctcgagcag ctgaagcttg catgcctgca
     2161 gggctatcaa ctttgtatag aaaagttggg ctgctgattc atatcccagt gaggtcatct
     2221 gaattttggg ggacctcaaa tagaaatcat atgggggaaa gaatgagcct agctcaaacc
     2281 agacaggctg agagcttatc ttgccaggac ataggctccc cggtgggtgg gggagatggc
     2341 cattgctgcc tttctttcct tccccactga atttaagcag atgcagttag tcactgtgca
     2401 aaggagtatg tgcaaatcta agtattcatg tttatgtgtg gttgtcaggt ctaaatcagc
     2461 caggcagtaa tttctctggg agcccaatgt aggcactaaa ttttgcactc aaccaccgaa
     2521 tggtccacat gccccttaat ctgtcttcct gcaggagggc tcccttggca ccatcctgcc
     2581 tgaacttggg gccagccttt cactggagcc ccttcccgtg aattatggcc tacagcatgg
     2641 gggctgaata gaaaaaaaaa aaccctccag aaattcaact ggaagggctg accggaattc
     2701 aacctaatgt tgagaatagc aggaaagctt ctattattct acttcattct atctgctatg
     2761 aggcagtgcg gtcgcctgga gtcgactgag cttaactcat tacgctggat tacggagtca
     2821 cagccatggt gcctgctaaa gccaaatatc tcactcctga gactcactac acttcctgat
     2881 aggatgcact tgcgtgtctc ctagtgacct taaacactaa taaaggagtc ataatatgac
     2941 ctagacaaga cctaggagcc aatcagaatg caaatgattg atgggagtgc ttttggttat
     3001 ctactgtttg tcctgacgct tggcgtggtt ctttgtatta tgattgtgtg ggcagtaggc
     3061 ttaaaaatac atacaggcat tgctttccac attgttgaag gttaatccta aggtgtggca
     3121 aagaaactat attttataga ctggaacctc atgggcacca gctagttttt gctcttcctc
     3181 aagtttgtac aaaaaagcag gctagacact agagggtata taatggaagc tcgacttcca
     3241 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag
     3301 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     3361 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
     3421 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
     3481 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
     3541 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     3601 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     3661 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     3721 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     3781 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     3841 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     3901 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     3961 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     4021 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     4081 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     4141 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     4201 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     4261 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     4321 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     4381 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     4441 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     4501 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     4561 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     4621 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     4681 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     4741 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     4801 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     4861 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     4921 gtgtaataat tctagagtca cccagctttc ttgtacaaag tggaaatgag tcttcggacc
     4981 tcgcgggggc cgcttaagcg gtggttaggg tttgtctgac gcggggggag ggggaaggaa
     5041 cgaaacactc tcattcggag gcggctcggg gtttggtctt ggtggccacg ggcacgcaga
     5101 agagcgccgc gatcctctta agcacccccc cgccctccgt ggaggcgggg gtttggtcgg
     5161 cgggtggtaa ctggcgggcc gctgactcgg gcgggtcgcg cgccccagag tgtgaccttt
     5221 tcggtctgct cgcagacccc cgggcggcgc cgccgcggcg gcgacgggct cgctgggtcc
     5281 taggctccat ggggaccgta tacgtggaca ggctctggag catccgcacg actgcggtga
     5341 tattaccgga gaccttctgc gggacgagcc gggtcacgcg gctgacgcgg agcgtccgtt
     5401 gggcgacaaa caccaggacg gggcacaggt acactatctt gtcacccgga ggcgcgaggg
     5461 actgcaggag cttcagggag tggcgcagct gcttcatccc cgtggcccgt tgctcgcgtt
     5521 tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa
     5581 ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg
     5641 gtcccaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct
     5701 gcagcgaccc gcttaaaagc ttgattcttc tgacacaaca gtctcgaact taagctgcag
     5761 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
     5821 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     5881 tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta
     5941 cagctcttaa ggctagagta cttaatacga ctcactatag gctagccacc atgacttcga
     6001 aagtttatga tccagaacaa aggaaacgga tgataactgg tccgcagtgg tgggccagat
     6061 gtaaacaaat gaatgttctt gattcattta ttaattatta tgattcagaa aaacatgcag
     6121 aaaatgctgt tattttttta catggtaacg cggcctcttc ttatttatgg cgacatgttg
     6181 tgccacatat tgagccagta gcgcggtgta ttataccaga ccttattggt atgggcaaat
     6241 caggcaaatc tggtaatggt tcttataggt tacttgatca ttacaaatat cttactgcat
     6301 ggtttgaact tcttaattta ccaaagaaga tcatttttgt cggccatgat tggggtgctt
     6361 gtttggcatt tcattatagc tatgagcatc aagataagat caaagcaata gttcacgctg
     6421 aaagtgtagt agatgtgatt gaatcatggg atgaatggcc tgatattgaa gaagatattg
     6481 cgttgatcaa atctgaagaa ggagaaaaaa tggttttgga gaataacttc ttcgtggaaa
     6541 ccatgttgcc atcaaaaatc atgagaaagt tagaaccaga agaatttgca gcatatcttg
     6601 aaccattcaa agagaaaggt gaagttcgtc gtccaacatt atcatggcct cgtgaaatcc
     6661 cgttagtaaa aggtggtaaa cctgacgttg tacaaattgt taggaattat aatgcttatc
     6721 tacgtgcaag tgatgattta ccaaaaatgt ttattgaatc ggacccagga ttcttttcca
     6781 atgctattgt tgaaggtgcc aagaagtttc ctaatactga atttgtcaaa gtaaaaggtc
     6841 ttcatttttc gcaagaagat gcacctgatg aaatgggaaa atatatcaaa tcgttcgttg
     6901 agcgagttct caaaaatgaa caataattct agagcggccg cttcgagcag acatgataag
     6961 atacattgat gagcaacttt attatacata gttgataatt cactcgacaa tcaacctctg
     7021 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7081 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7141 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7201 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7261 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7321 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7381 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     7441 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7501 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7561 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     7621 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7681 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     7741 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7801 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7861 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     7921 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7981 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8041 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8101 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     8161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8221 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8281 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8341 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8401 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8461 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8521 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8641 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8701 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8761 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8821 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8881 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8941 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9001 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9061 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9121 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9181 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9241 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9301 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9361 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9421 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9481 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9541 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9601 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9661 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9721 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9781 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9841 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9901 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9961 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10021 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10081 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10141 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10201 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10261 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10321 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10381 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10441 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10501 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10561 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10621 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10681 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10741 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10801 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10861 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10921 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10981 caat
//
