LOCUS       sequence-273257-       10161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ATP measurement, Tol2 transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Yaginuma H,  Okada Y
  TITLE     Live cell imaging of metabolic heterogeneity by quantitative 
            fluorescent ATP indicator protein, QUEEN-37C
  JOURNAL   bioRxiv 2021.10.08.463131
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.10.08.463131"
COMMENT     Sequence Label: pT2MP.EF-QUE37C
FEATURES             Location/Qualifiers
     source          1..10161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1192..1272
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1274..1283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1640..1891
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1828..1849
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1955..1976)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2216..2235)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2479..2703
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2750..2773)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3147..3166
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3231..3830
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3231..3250)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3727..3747
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3917..4141
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4169..4185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4193..4209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4217..4223,4224..4241,4242..4247))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4262..4283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(5599..5736,5737..5748)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(5767..5785)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5806..5822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5830..5846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5854..5860,5861..5878,5879..5884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5899..5920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6037..6054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6208..6796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6288..6307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6967..7758,7759..7827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7590..7609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7828..7932)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7958..8413)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8095..8116)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8307..8326
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8540..8562
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8554..8571
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8555..8571
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8578..8597
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8578..8596
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(8610..8621,8622..8809)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    10078..10126
                     /note="synthetic polyadenylation signal"
     promoter        join(10161,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattaa agctagcgct
     1201 accggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
     1261 ggatccaccg gtcgccacca tggtgaaaac tgtgaaagtg aatataacaa cccctgatgg
     1321 gccagtctac gacgctgata tcgagatggt gtccgtgcgg gccgagagtg gtgatctcgg
     1381 catcctcccc ggtcacattc ccacaaaggc cccactgaag atcggagctg tgcggctgaa
     1441 gaaggacggc caaacagagg atgtcgcagt ctcaggcggc actgttgaag tgcggcctga
     1501 ccacgttacc attaatgctc aagccgctga acgggcggag gacattgacg tcctccgcgc
     1561 caaagcggcg aaagagcggg cggagcgccg cctgcaaagc cagacccgtt acaacagcca
     1621 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1681 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1741 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
     1801 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1861 gatcactctc ggcatggacg agctgtacaa gggtggcacc ggagggagca tggtgagcaa
     1921 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1981 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     2041 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     2101 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     2161 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     2221 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     2281 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     2341 caacctgggt gacatcgact tcaaacgggc cgaactggcg ttaaaacgcg ccatgaaccg
     2401 tttgagcgtt gcggaagata agtgagcggc cgctcgataa gcttgatcga tgatctagag
     2461 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     2521 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     2581 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     2641 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     2701 tggcttctga ggcggaaaga acctgcagct tttcccaagg cagtctggag catgcgcttt
     2761 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     2821 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     2881 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     2941 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     3001 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     3061 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     3121 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     3181 gctgttctcc tcttcctcat ctccgggcct ttcgacctgt ggccacaacc atgaccgagt
     3241 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     3301 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     3361 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     3421 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     3481 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     3541 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     3601 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     3661 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     3721 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     3781 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     3841 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg atctagagct
     3901 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3961 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4021 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4081 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4141 gctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4201 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4261 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4321 acctgtcgtg ccagcggatc cctagaggga cagccccccc ccaaagcccc cagggatgta
     4381 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     4441 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     4501 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     4561 ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag aatcatagaa
     4621 cggcctgggt tgcaaaggag cacagtgctc atccagatcc aaccccctgc tatgtgcagg
     4681 gtcatcaacc agcagcccag gctgcccaga gccacatcca gcctggcctt gaatgcctgc
     4741 agggatgggg catccacagc ctccttgggc aacctgttca gtgcgtcacc accctctggg
     4801 ggaaaaactg cctcctcata tccaacccaa acctcccctg tctcagtgta aagccattcc
     4861 cccttgtcct atcaaggggg agtttgctgt gacattgttg gtctggggtg acacatgttt
     4921 gccaattcag tgcatcacgg agaggcagat cttggggata aggaagtgca ggacagcatg
     4981 gacgtgggac atgcaggtgt tgagggctct gggacactct ccaagtcaca gcgttcagaa
     5041 cagccttaag gataagaaga taggatagaa ggacaaagag caagttaaaa cccagcatgg
     5101 agaggagcac aaaaaggcca cagacactgc tggtccctgt gtctgagcct gcatgtttga
     5161 tggtgtctgg atgcaagcag aaggggtgga agagcttgcc tggagagata cagctgggtc
     5221 agtaggactg ggacaggcag ctggagaatt gccatgtaga tgttcataca atcgtcaaat
     5281 catgaaggct ggaaaagccc tccaagatcc ccaagaccaa ccccaaccca cccaccgtgc
     5341 ccactggcca tgtccctcag tgccacatcc ccacagttct tcatcacctc cagggacggt
     5401 gaccccccca cctccgtggg cagctgtgcc actgcagcac cgctctttgg agaaggtaaa
     5461 tcttgctaaa tccagcccga ccctcccctg gcacaacgta aggccattat ctctcatcca
     5521 actccaggac ggagtcagtg aggatggggc tctagccgga ccgacgcgtc tcgataggga
     5581 taacagggta attgatccaa tactcaagta caattttaat ggagtacttt tttactttta
     5641 ctcaagtaag attctagcca gatactttta cttttaattg agtaaaattt tccctaagta
     5701 cttgtacttt cacttgagta aaatttttga gtacttttta cacctctgga gctccagctt
     5761 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     5821 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5881 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5941 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6001 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7741 caccagcgtt tctgggtgag caaaaacggg aaggcaaaat gccgcaaaaa agggaataag
     7801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7921 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     7981 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     8041 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     8101 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     8161 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     8221 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     8281 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     8341 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     8401 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8461 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8521 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     8581 tacgactcac tatagggcga attgggtacc agaggtgtaa agtacttgag taattttact
     8641 tgattactgt acttaagtat tatttttggg gatttttact ttacttgagt acaattaaaa
     8701 atcaatactt ttacttttac ttaattacat ttttttagaa aaaaaagtac tttttactcc
     8761 ttacaatttt atttacagtc aaaaagtact tattttttgg agatcacttg tcgagtaggg
     8821 ataacagggt aatgatcccc cgggctgcag tcgcgacgcg tcctagaggg acagcccccc
     8881 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     8941 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     9001 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     9061 tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt
     9121 tagaatgaca gaatcataga acggcctggg ttgcaaagga gcacagtgct catccagatc
     9181 caaccccctg ctatgtgcag ggtcatcaac cagcagccca ggctgcccag agccacatcc
     9241 agcctggcct tgaatgcctg cagggatggg gcatccacag cctccttggg caacctgttc
     9301 agtgcgtcac caccctctgg gggaaaaact gcctcctcat atccaaccca aacctcccct
     9361 gtctcagtgt aaagccattc ccccttgtcc tatcaagggg gagtttgctg tgacattgtt
     9421 ggtctggggt gacacatgtt tgccaattca gtgcatcacg gagaggcaga tcttggggat
     9481 aaggaagtgc aggacagcat ggacgtggga catgcaggtg ttgagggctc tgggacactc
     9541 tccaagtcac agcgttcaga acagccttaa ggataagaag ataggataga aggacaaaga
     9601 gcaagttaaa acccagcatg gagaggagca caaaaaggcc acagacactg ctggtccctg
     9661 tgtctgagcc tgcatgtttg atggtgtctg gatgcaagca gaaggggtgg aagagcttgc
     9721 ctggagagat acagctgggt cagtaggact gggacaggca gctggagaat tgccatgtag
     9781 atgttcatac aatcgtcaaa tcatgaaggc tggaaaagcc ctccaagatc cccaagacca
     9841 accccaaccc acccaccgtg cccactggcc atgtccctca gtgccacatc cccacagttc
     9901 ttcatcacct ccagggacgg tgaccccccc acctccgtgg gcagctgtgc cactgcagca
     9961 ccgctctttg gagaaggtaa atcttgctaa atccagcccg accctcccct ggcacaacgt
    10021 aaggccatta tctctcatcc aactccagga cggagtcagt gaggatgggg ctctaggaat
    10081 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtggtcg acattgatta
    10141 ttgactagat catcgcgtga g
//
