LOCUS       sequence-27327-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe
            T
  TITLE     Analysis of protein interactions with two-hybrid system in cultured 
            insect cells.
  JOURNAL   Anal Biochem. 2009 Sep 15;392(2):180-2. doi: 
            10.1016/j.ab.2009.05.033.
  PUBMED    19481053
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.05"; journalName: "Anal Biochem"; date: 
            "2009-09-15- 15"; volume: "392"; issue: "2"; pages: "180-2"
COMMENT     Sequence Label: pUAS-Luc
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6..22
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(56..72)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(56..72)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    89..182
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    351..375
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(498..516)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc
       61 actagttcta gagcggccgc ccgctagacg gagtactgtc ctccgagcgg agtactgtcc
      121 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      181 gatcacgttc attctgttta aatatgcgac accgctggcg ggaagcagaa ttatttgaaa
      241 cgcaatacga agcagacgaa gagagttcag tgcaaactga aaacaagttt ttcgacgatt
      301 tatttattgt ttattgtgaa gaagtatcta cagtgattaa tattcaaaat acaagtttgt
      361 acaaaaaagc aggcttcgaa ggagatagaa ccaattctct aaggaaatac ttaaccatgg
      421 tcgagatcca aatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc
      481 tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
      541 ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact
      601 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
      661 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
      721 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
      781 gtatgaacat ttcgcagcct accgtanngt ttgtttccaa aaggggttgc aaaaaatttt
//
