LOCUS       sequence-273272-       11554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Tiam DH/PH-tgRFPt-Nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11554)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 11554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: mTiam1(64-437)-tgRFPt-SSPB WT
FEATURES             Location/Qualifiers
     source          1..11554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2616..2928
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2847..2867
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2945..2961
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3003..3036)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        3152..3455
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3456..3659
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4871..5599
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     CDS             5675..6004
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     protein_bind    6017..6050
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6123..6144)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7250..7978
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     CDS             8054..8383
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     misc_feature    8445..9031
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8498..8518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8916..8927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9034..9050)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             9560..9740
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9802..10390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9882..9901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10561..11352,11353..11421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11184..11203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11422..11526)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgcgct ctagagatcc gacgcgccat ctctaggccc
     2641 gcgccggccc cctcgcacag acttgtggga gaagctcggc tactcccctg ccccggttaa
     2701 tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat
     2761 ctgttctttt taatactagc tacattttac atgataggct tggatttcta taagagatac
     2821 aaatactaaa ttattatttt aaaaaacagc acaaaaggaa actcacccta actgtaaagt
     2881 aattgtgtgt tttgagacta taaatatccc ttggagaaaa gccttgttaa cgcgcggtga
     2941 ccctcgaggt cgacggtatc gataagctcg cttcacgaga ttccagcagg tcgagggacc
     3001 taataacttc gtatagcata cattatacga agttatatta agggttccaa gcttaagcgg
     3061 ccgcgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
     3121 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3181 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3241 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3301 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     3361 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     3421 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     3481 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     3541 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     3601 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
     3661 gtttagtgaa ccgtcagatc cgctagaatt ctgcagtcga cggtaccgcg ggcccgccac
     3721 catgcgacag ctgtcggatg cggataagct gcgcaaggtg atctgtgaac tgctggagac
     3781 tgaacgcacc tatgtgaaag acttaaactg cctcatggag agatacctga agccccttca
     3841 gaaggagacc tttctcaccc aggatgagct tgatgtactt tttggaaatt taaccgaaat
     3901 ggtggagttt caagtcgagt tccttaagac tctagaagat ggagtaagac tggtccctga
     3961 cttggaaaag ctggagaagg ttgaccagtt caagaaagtg ctcttctctc tggggggatc
     4021 cttcctgtac tacgcggacc gcttcaagct ctacagtgcc ttctgtgcga gccacacaaa
     4081 agtccccaag gtcctggtga aagccaagac ggacacagcc ttcaaggcgt tcctcgatgc
     4141 ccagaacccg aggcagcagc actcatccac gctggagtct tacctcatca agcccatcca
     4201 gagggtcctc aagtacccgc ttctgctcag ggagctgttt gcgctgaccg atgcggagag
     4261 cgaggaacac taccacctgg acgtggccat caagactatg aacaaggtcg ccagtcacat
     4321 caatgagatg cagaagatcc acgaagagtt tggtgccgtg tttgaccagc tgattgctga
     4381 gcagacggga gagaagaaag aggttgcaga tctgagcatg ggtgacctgc tcttgcacac
     4441 cagcgtcatc tggctgaacc cacccgcctc actgggaaag tggaaaaagg agccagaatt
     4501 agcagccttc gtcttcaaaa cggccgtggt ccttgtatat aaagacggtt ccaagcagaa
     4561 gaagaaactt gttggctctc acaggctgtc aatctacgag gagtgggacc ccttccggtt
     4621 tcgccacatg atccctaccg aagctttgca ggtccgagct ctgcccagtg cagatgcaga
     4681 ggcaaatgcg gtatgcgaaa ttgtccacgt gaaatcagag tcagaaggga ggcccgagcg
     4741 ggttttccac ctctgctgca gttccccaga gagcagaaag gactttctga agtctgtgca
     4801 ttcgatcctg cgagataaac acagaagaca gctcctcaaa acggaagaat tcggtagtgg
     4861 aagtggttcc gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta
     4921 catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc
     4981 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     5041 cttcgacatc ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca
     5101 gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac
     5161 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     5221 cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca
     5281 gaagaaaaca ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct
     5341 ggaaggcaga accgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt
     5401 caagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta
     5461 tgtggaccac agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca
     5521 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaatgg
     5581 catggacgag ctgtacaagt ccggactcag atctcgagct caagcttcga acgaattcgg
     5641 cattgatctg agcggcctga ccctgcagga attcagctcc ccgaaacgcc ctaagctgct
     5701 gcgtgaatat tacgattggc tggttgataa cagctttacc ccatatctgg tggtggatgc
     5761 cacatacctg ggcgtgaacg tgcccgtgga gtatgtgaaa gacggtcaga tcgtgctgaa
     5821 tctgtctgca agtgcgaccg gcaacctgca actgacaaat gattttatcc agttcaacgc
     5881 ccgctttaag ggcgtgtctc gtgaactgta tatcccgatg ggtgccgctc tggccattta
     5941 cgctcgcgag aacggcgatg gtgtgatgtt cgaaccagaa gaaatctatg acgagctgaa
     6001 tattggttaa acatgtataa cttcgtataa tgtatgctat acgaagttat taggtccctc
     6061 gacgaattgc ctgcaggtta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     6121 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt krrsgcacct atgtgaaaga
     6181 cttaaactgc ctcatggaga gatacctgaa gccccttcag aaggagacct ttctcaccca
     6241 ggatgagctt gatgtacttt ttggaaattt aaccgaaatg gtggagtttc aagtcgagtt
     6301 ccttaagact ctagaagatg gagtaagact ggtccctgac ttggaaaagc tggagaaggt
     6361 tgaccagttc aagaaagtgc tcttctctct ggggggatcc ttcctgtact acgcggaccg
     6421 cttcaagctc tacagtgcct tctgtgcgag ccacacaaaa gtccccaagg tcctggtgaa
     6481 agccaagacg gacacagcct tcaaggcgtt cctcgatgcc cagaacccga ggcagcagca
     6541 ctcatccacg ctggagtctt acctcatcaa gcccatccag agggtcctca agtacccgct
     6601 tctgctcagg gagctgtttg cgctgaccga tgcggagagc gaggaacact accacctgga
     6661 cgtggccatc aagactatga acaaggtcgc cagtcacatc aatgagatgc agaagatcca
     6721 cgaagagttt ggtgccgtgt ttgaccagct gattgctgag cagacgggag agaagaaaga
     6781 ggttgcagat ctgagcatgg gtgacctgct cttgcacacc agcgtcatct ggctgaaccc
     6841 acccgcctca ctgggaaagt ggaaaaagga gccagaatta gcagccttcg tcttcaaaac
     6901 ggccgtggtc cttgtatata aagacggttc caagcagaag aagaaacttg ttggctctca
     6961 caggctgtca atctacgagg agtgggaccc cttccggttt cgccacatga tccctaccga
     7021 agctttgcag gtccgagctc tgcccagtgc agatgcagag gcaaatgcgg tatgcgaaat
     7081 tgtccacgtg aaatcagagt cagaagggag gcccgagcgg gttttccacc tctgctgcag
     7141 ttccccagag agcagaaagg actttctgaa gtctgtgcat tcgatcctgc gagataaaca
     7201 cagaagacag ctcctcaaaa cggaagaatt cggtagtgga agtggttccg tgtctaaggg
     7261 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa
     7321 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     7381 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
     7441 cttcatgtac ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa
     7501 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
     7561 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
     7621 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     7681 ggccaacacc gagatgctgt accccgctga cggcggcctg gaaggcagaa ccgacatggc
     7741 cctgaagctc gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa
     7801 gaaacccgct aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag
     7861 aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata
     7921 ctgcgacctc cctagcaaac tggggcacaa acttaatggc atggacgagc tgtacaagtc
     7981 cggactcaga tctcgagctc aagcttcgaa cgaattcggc attgatctga gcggcctgac
     8041 cctgcaggaa ttcagctccc cgaaacgccc taagctgctg cgtgaatatt acgattggct
     8101 ggttgataac agctttaccc catatctggt ggtggatgcc acatacctgg gcgtgaacgt
     8161 gcccgtggag tatgtgaaag acggtcagat cgtgctgaat ctgtctgcaa gtgcgaccgg
     8221 caacctgcaa ctgacaaatg attttatcca gttcaacgcc cgctttaagg gcgtgtctcg
     8281 tgaactgtat atcccgatgg gtgccgctct ggccatttac gctcgcgaga acggcgatgg
     8341 tgtgatgttc gaaccagaag aaatctatga cgagctgaat attggttaaa catgtttaag
     8401 ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt
     8461 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8521 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8581 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8641 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8701 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8761 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8821 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     8881 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8941 cttcccggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9001 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     9061 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     9121 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     9181 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9241 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     9301 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     9361 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     9421 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     9481 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     9541 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9601 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9661 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9721 agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    10021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    10261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    11041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11521 tccgcgcaca tttccccgaa aagtgccacc tgac
//
