LOCUS       sequence-273274-       10150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PE2 and Tetra-com modified pegRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10150)
  TITLE     Lentiviral capsid delivery of SpCas9 RNP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-PE2-pegRNA-tetra-com vector
FEATURES             Location/Qualifiers
     source          1..10150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        174..553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        554..757
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     708..728
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     799..818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        799..817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             867..887
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(888..3401,3402..3404,3405..4988)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             888..4988
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7161..7181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7190..7207
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7230..7247)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7236..7460
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7531..7547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7531..7547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7544..7566)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7555..7571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7579..7585,7586..7603,7604..7609))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7624..7645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7762..7779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7933..8521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8013..8032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8692..9483,9484..9552))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9315..9334
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9553..9657)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9736..9755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(9910..10150,1..8)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9910..9930
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10081..10100
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaaacaccgg gagacgctcg agcgtctccg tttgagagct aggccctgaa tgcctgcgag
       61 catcccacgg cctagcaagt tcaaataagg ctagtccgtt atcaacttga aaaagtggca
      121 ccgagtcggt gctttttttg tacatcgcga tgtacgggcc agatatacgc gttgacattg
      181 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      241 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      301 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      361 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      421 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      481 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      541 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      601 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      661 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      721 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
      781 ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga
      841 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
      901 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      961 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     1021 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     1081 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1141 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1201 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1261 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1321 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1381 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1441 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1501 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     1561 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1621 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1681 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1741 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1801 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1861 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1921 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     1981 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     2041 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     2101 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2161 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2221 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2281 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2341 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2401 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2461 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2521 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2581 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2641 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2701 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2761 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2821 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     2881 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     2941 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     3001 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     3061 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3121 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3181 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3241 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3301 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3361 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3421 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3481 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3541 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3601 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3661 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3721 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     3781 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     3841 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     3901 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     3961 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     4021 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     4081 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4141 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4201 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4261 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4321 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4381 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4441 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4501 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4561 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4621 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4681 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4741 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     4801 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     4861 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     4921 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     4981 gaggtgactc tggaggatct agcggaggat cctctggcag cgagacacca ggaacaagcg
     5041 agtcagcaac accagagagc agtggcggca gcagcggcgg cagcagcacc ctaaatatag
     5101 aagatgagta tcggctacat gagacctcaa aagagccaga tgtttctcta gggtccacat
     5161 ggctgtctga ttttcctcag gcctgggcgg aaaccggggg catgggactg gcagttcgcc
     5221 aagctcctct gatcatacct ctgaaagcaa cctctacccc cgtgtccata aaacaatacc
     5281 ccatgtcaca agaagccaga ctggggatca agccccacat acagagactg ttggaccagg
     5341 gaatactggt accctgccag tccccctgga acacgcccct gctacccgtt aagaaaccag
     5401 ggactaatga ttataggcct gtccaggatc tgagagaagt caacaagcgg gtggaagaca
     5461 tccaccccac cgtgcccaac ccttacaacc tcttgagcgg gctcccaccg tcccaccagt
     5521 ggtacactgt gcttgattta aaggatgcct ttttctgcct gagactccac cccaccagtc
     5581 agcctctctt cgcctttgag tggagagatc cagagatggg aatctcagga caattgacct
     5641 ggaccagact cccacagggt ttcaaaaaca gtcccaccct gtttaatgag gcactgcaca
     5701 gagacctagc agacttccgg atccagcacc cagacttgat cctgctacag tacgtggatg
     5761 acttactgct ggccgccact tctgagctag actgccaaca aggtactcgg gccctgttac
     5821 aaaccctagg gaacctcggg tatcgggcct cggccaagaa agcccaaatt tgccagaaac
     5881 aggtcaagta tctggggtat cttctaaaag agggtcagag atggctgact gaggccagaa
     5941 aagagactgt gatggggcag cctactccga agacccctcg acaactaagg gagttcctag
     6001 ggaaggcagg cttctgtcgc ctcttcatcc ctgggtttgc agaaatggca gcccccctgt
     6061 accctctcac caaaccgggg actctgttta attggggccc agaccaacaa aaggcctatc
     6121 aagaaatcaa gcaagctctt ctaactgccc cagccctggg gttgccagat ttgactaagc
     6181 cctttgaact ctttgtcgac gagaagcagg gctacgccaa aggtgtccta acgcaaaaac
     6241 tgggaccttg gcgtcggccg gtggcctacc tgtccaaaaa gctagaccca gtagcagctg
     6301 ggtggccccc ttgcctacgg atggtagcag ccattgccgt actgacaaag gatgcaggca
     6361 agctaaccat gggacagcca ctagtcattc tggcccccca tgcagtagag gcactagtca
     6421 aacaaccccc cgaccgctgg ctttccaacg cccggatgac tcactatcag gccttgcttt
     6481 tggacacgga ccgggtccag ttcggaccgg tggtagccct gaacccggct acgctgctcc
     6541 cactgcctga ggaagggctg caacacaact gccttgatat cctggccgaa gcccacggaa
     6601 cccgacccga cctaacggac cagccgctcc cagacgccga ccacacctgg tacacggatg
     6661 gaagcagtct cttacaagag ggacagcgta aggcgggagc tgcggtgacc accgagaccg
     6721 aggtaatctg ggctaaagcc ctgccagccg ggacatccgc tcagcgggct gaactgatag
     6781 cactcaccca ggccctaaag atggcagaag gtaagaagct aaatgtttat actgatagcc
     6841 gttatgcttt tgctactgcc catatccatg gagaaatata cagaaggcgt gggtggctca
     6901 catcagaagg caaagagatc aaaaataaag acgagatctt ggccctacta aaagccctct
     6961 ttctgcccaa aagacttagc ataatccatt gtccaggaca tcaaaaggga cacagcgccg
     7021 aggctagagg caaccggatg gctgaccaag cggcccgaaa ggcagccatc acagagactc
     7081 cagacacctc taccctcctc atagaaaatt catcaccctc tggcggctca aaaagaaccg
     7141 ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctaaccggtc atcatcacca
     7201 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7261 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7321 ttcctaataa aatgagaaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7381 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7441 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc
     7501 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7561 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc
     7621 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7681 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7741 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7801 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7861 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7921 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7981 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8041 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8101 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8161 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8221 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8281 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8341 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8401 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8461 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8521 agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa actcacgtta
     8581 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8641 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8701 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8761 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8821 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8881 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8941 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9001 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9061 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9121 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9181 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9241 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9301 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9361 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9421 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9481 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9541 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9601 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9661 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct
     9721 agggtctact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9781 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     9841 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     9901 cgaaagcttg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     9961 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
    10021 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
    10081 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
    10141 tggaaaggac
//
