LOCUS       sequence-273275-        9913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Target-ACEmax expression vector (pSI-625).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto
            S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, 
            Nureki O, Tomita M, Aburatani H, Yachie N
  TITLE     Base editors for simultaneous introduction of C-to-T and A-to-G 
            mutations.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 
            10.1038/s41587-020-0509-0.
  PUBMED    32483365
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 
            10.1038/s41587-020-0509-0."
COMMENT     Sequence Label: pCMV-Target-ACEmax
FEATURES             Location/Qualifiers
     source          1..9913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     644..663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        644..662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             712..732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1921..1944,1945..1947,1948..6021)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1921..6021
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6229..6294
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6928..6948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6955..7209
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     primer_bind     complement(7235..7252)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7241..7465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7536..7552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7536..7552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7549..7571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7560..7576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7584..7590,7591..7608,7609..7614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7629..7650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7767..7784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7938..8526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8018..8037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8697..9488,9489..9557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9320..9339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9558..9662)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9741..9760)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctggtttagt gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata
      661 gggagagccg ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag
      721 aagcggaaag tctctgaagt cgagtttagc cacgagtatt ggatgaggca cgcactgacc
      781 ctggcaaagc gagcatggga tgaaagagaa gtccccgtgg gcgccgtgct ggtgcacaac
      841 aatagagtga tcggagaggg atggaacagg ccaatcggcc gccacgaccc taccgcacac
      901 gcagagatca tggcactgag gcagggaggc ctggtcatgc agaattaccg cctgatcgat
      961 gccaccctgt atgtgacact ggagccatgc gtgatgtgcg caggagcaat gatccacagc
     1021 aggatcggaa gagtggtgtt cggagcacgg gacgccaaga ccggcgcagc aggctccctg
     1081 atggatgtgc tgcaccaccc cggcatgaac caccgggtgg agatcacaga gggaatcctg
     1141 gcagacgagt gcgccgccct gctgagcgat ttctttagaa tgcggagaca ggagatcaag
     1201 gcccagaaga aggcacagag ctccaccgac tctggaggat ctagcggagg atcctctgga
     1261 agcgagacac caggcacaag cgagtccgcc acaccagaga gctccggcgg ctcctccgga
     1321 ggatcctctg aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc
     1381 aagagggcac gcgatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga
     1441 gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa
     1501 attatggccc tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc
     1561 ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc
     1621 ggccgcgtgg tgtttggcgt gaggaacgca aaaaccggcg ccgcaggctc cctgatggac
     1681 gtgctgcact accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat
     1741 gaatgtgccg ccctgctgtg ctatttcttt cggatgccta gacaggtgtt caatgctcag
     1801 aagaaggccc agagctccac cgactccgga ggatctagcg gaggctcctc tggctctgag
     1861 acacctggca caagcgagag cgcaacacct gaaagcagcg ggggcagcag cggggggtca
     1921 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     1981 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     2041 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     2101 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     2161 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     2221 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     2281 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     2341 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     2401 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     2461 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     2521 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     2581 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     2641 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     2701 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     2761 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     2821 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     2881 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     2941 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     3001 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     3061 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     3121 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     3181 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     3241 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     3301 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     3361 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     3421 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     3481 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     3541 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     3601 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     3661 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     3721 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     3781 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     3841 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     3901 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     3961 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     4021 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     4081 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     4141 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     4201 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     4261 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     4321 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     4381 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     4441 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     4501 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     4561 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     4621 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     4681 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     4741 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     4801 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     4861 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     4921 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     4981 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     5041 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     5101 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     5161 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     5221 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     5281 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     5341 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     5401 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     5461 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     5521 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     5581 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     5641 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     5701 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     5761 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     5821 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     5881 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     5941 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     6001 ctgtctcagc tgggaggtga cggcggagga ggaactggag gaggaggatc agcagaatat
     6061 gtgagagcac tgtttgactt taatgggaat gacgaagaag acctgccctt caagaagggc
     6121 gacatcctgc ggatcagaga taagcctgag gagcagtggt ggaacgccga ggactctgag
     6181 ggcaagagag gcatgatcct ggtgccctac gtggagaagt acagcggcga ttataaggac
     6241 cacgatggcg actacaagga ccacgacatc gactacaagg atgacgatga caagtctggc
     6301 atgacagatg ccgagtatgt gagaatccac gagaagctgg acatctacac cttcaagaag
     6361 cagtttttca acaataagaa gagcgtgtcc cacagatgct acgtgctgtt tgagctgaag
     6421 agaaggggcg agcggagagc ctgtttttgg ggctacgccg tgaacaagcc ccagtctggc
     6481 acagagcggg gcatccacgc cgagatcttc tccatccgga aggtggagga gtacctgcgg
     6541 gacaacccag gccagttcac catcaactgg tacagctcct ggtccccatg tgccgactgt
     6601 gccgagaaga tcctggagtg gtacaaccag gagctgcggg gcaacggcca caccctgaag
     6661 atctgggcct gcaagctgta ctatgagaag aatgccagaa accagatcgg cctgtggaac
     6721 ctgagagaca atggcgtggg cctgaacgtg atggtgtctg agcactacca gtgctgtagg
     6781 aagatcttca tccagtcttc ccacaaccag ctgaatgaga acagatggct ggagaagaca
     6841 ctgaagcggg ccgagaagtg gagaagcgag ctgtccatca tgatccaggt gaagatcctg
     6901 cacaccacca agtctccagc cgtgggccca aagaagaagc gcaaggtggg caccatgacc
     6961 aacctgtctg acatcatcga gaaggagaca ggcaagcagc tggtaatcca ggagagcatc
     7021 ctgatgctgc ctgaggaggt ggaggaggta atcggcaaca agcctgagag cgatatcctg
     7081 gtgcacaccg cctatgatga gagcacagac gagaacgtga tgctgctgac cagcgacgcc
     7141 cctgagtaca aaccttgggc actggtcatt caggatagta acggggagaa caaaatcaaa
     7201 atgctgtgat aatgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     7261 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7321 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7381 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     7441 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata
     7501 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7561 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag
     7621 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7681 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttctg
     7801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcatgg
     7861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8401 ctgctgaagc cagttacctt ctgaaaaaga gttggtagct cttgatccgg caaacaaacc
     8461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8521 tctcaagaag atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca
     8581 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8641 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8701 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8761 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8821 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8881 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8941 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9001 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9061 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9121 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9181 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9241 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9301 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9361 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9421 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9481 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9541 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9601 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9661 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat
     9721 cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     9781 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     9841 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     9901 cttcgcgatg tac
//
