LOCUS       sequence-273277-       10146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PE2 and ST2-com modified pegRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10146)
  TITLE     Lentiviral capsid delivery of SpCas9 RNP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-PE2-pegRNA-ST2-com-vector
FEATURES             Location/Qualifiers
     source          1..10146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        253..501
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     253..273
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     424..443
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        663..1042
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1043..1246
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1197..1217
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1288..1307
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1288..1306
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1356..1376
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1377..3890,3891..3893,3894..5477)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1377..5477
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7650..7670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7679..7696
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7719..7736)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7725..7949
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8020..8036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8020..8036)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8033..8055)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8044..8060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8068..8074,8075..8092,8093..8098))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8113..8134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8251..8268)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8422..9010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8502..8521)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9181..9972,9973..10041))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9804..9823
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10042..10146)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
       61 cctagggtct actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      121 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      181 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      241 tcgcgaaagc ttgagggcct atttcccatg attccttcat atttgcatat acgatacaag
      301 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
      361 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      421 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      481 ttgtggaaag gacgaaacac cgggagacgc tcgagcgtct ccgtttgaga gctagaaata
      541 gcaagttcaa ataaggctag tccgttatca acttggctga atgcctgcga gcatcccacc
      601 caagtggcac cgagtcggtg ctttttttgt acatcgcgat gtacgggcca gatatacgcg
      661 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      721 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      781 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      841 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      901 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      961 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1021 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     1081 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1141 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1201 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     1261 tcagatccgc tagagatccg cggccgctaa tacgactcac tatagggaga gccgccacca
     1321 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtcgaca
     1381 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
     1441 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     1501 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
     1561 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     1621 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     1681 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     1741 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     1801 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     1861 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     1921 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     1981 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     2041 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
     2101 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     2161 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     2221 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     2281 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     2341 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     2401 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     2461 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     2521 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     2581 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     2641 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     2701 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     2761 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     2821 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     2881 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg
     2941 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     3001 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     3061 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     3121 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     3181 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     3241 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     3301 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     3361 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     3421 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     3481 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     3541 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     3601 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     3661 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     3721 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     3781 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     3841 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc
     3901 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga
     3961 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     4021 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg
     4081 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     4141 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     4201 acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     4261 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     4321 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     4381 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     4441 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     4501 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     4561 tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg
     4621 tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     4681 gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa
     4741 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     4801 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     4861 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     4921 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     4981 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     5041 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     5101 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     5161 agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     5221 ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca
     5281 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg
     5341 ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc
     5401 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
     5461 ctcagctggg aggtgactct ggaggatcta gcggaggatc ctctggcagc gagacaccag
     5521 gaacaagcga gtcagcaaca ccagagagca gtggcggcag cagcggcggc agcagcaccc
     5581 taaatataga agatgagtat cggctacatg agacctcaaa agagccagat gtttctctag
     5641 ggtccacatg gctgtctgat tttcctcagg cctgggcgga aaccgggggc atgggactgg
     5701 cagttcgcca agctcctctg atcatacctc tgaaagcaac ctctaccccc gtgtccataa
     5761 aacaataccc catgtcacaa gaagccagac tggggatcaa gccccacata cagagactgt
     5821 tggaccaggg aatactggta ccctgccagt ccccctggaa cacgcccctg ctacccgtta
     5881 agaaaccagg gactaatgat tataggcctg tccaggatct gagagaagtc aacaagcggg
     5941 tggaagacat ccaccccacc gtgcccaacc cttacaacct cttgagcggg ctcccaccgt
     6001 cccaccagtg gtacactgtg cttgatttaa aggatgcctt tttctgcctg agactccacc
     6061 ccaccagtca gcctctcttc gcctttgagt ggagagatcc agagatggga atctcaggac
     6121 aattgacctg gaccagactc ccacagggtt tcaaaaacag tcccaccctg tttaatgagg
     6181 cactgcacag agacctagca gacttccgga tccagcaccc agacttgatc ctgctacagt
     6241 acgtggatga cttactgctg gccgccactt ctgagctaga ctgccaacaa ggtactcggg
     6301 ccctgttaca aaccctaggg aacctcgggt atcgggcctc ggccaagaaa gcccaaattt
     6361 gccagaaaca ggtcaagtat ctggggtatc ttctaaaaga gggtcagaga tggctgactg
     6421 aggccagaaa agagactgtg atggggcagc ctactccgaa gacccctcga caactaaggg
     6481 agttcctagg gaaggcaggc ttctgtcgcc tcttcatccc tgggtttgca gaaatggcag
     6541 cccccctgta ccctctcacc aaaccgggga ctctgtttaa ttggggccca gaccaacaaa
     6601 aggcctatca agaaatcaag caagctcttc taactgcccc agccctgggg ttgccagatt
     6661 tgactaagcc ctttgaactc tttgtcgacg agaagcaggg ctacgccaaa ggtgtcctaa
     6721 cgcaaaaact gggaccttgg cgtcggccgg tggcctacct gtccaaaaag ctagacccag
     6781 tagcagctgg gtggccccct tgcctacgga tggtagcagc cattgccgta ctgacaaagg
     6841 atgcaggcaa gctaaccatg ggacagccac tagtcattct ggccccccat gcagtagagg
     6901 cactagtcaa acaacccccc gaccgctggc tttccaacgc ccggatgact cactatcagg
     6961 ccttgctttt ggacacggac cgggtccagt tcggaccggt ggtagccctg aacccggcta
     7021 cgctgctccc actgcctgag gaagggctgc aacacaactg ccttgatatc ctggccgaag
     7081 cccacggaac ccgacccgac ctaacggacc agccgctccc agacgccgac cacacctggt
     7141 acacggatgg aagcagtctc ttacaagagg gacagcgtaa ggcgggagct gcggtgacca
     7201 ccgagaccga ggtaatctgg gctaaagccc tgccagccgg gacatccgct cagcgggctg
     7261 aactgatagc actcacccag gccctaaaga tggcagaagg taagaagcta aatgtttata
     7321 ctgatagccg ttatgctttt gctactgccc atatccatgg agaaatatac agaaggcgtg
     7381 ggtggctcac atcagaaggc aaagagatca aaaataaaga cgagatcttg gccctactaa
     7441 aagccctctt tctgcccaaa agacttagca taatccattg tccaggacat caaaagggac
     7501 acagcgccga ggctagaggc aaccggatgg ctgaccaagc ggcccgaaag gcagccatca
     7561 cagagactcc agacacctct accctcctca tagaaaattc atcaccctct ggcggctcaa
     7621 aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc taaccggtca
     7681 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     7741 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     7801 cactgtcctt tcctaataaa atgagaaaat tgcatcgcat tgtctgagta ggtgtcattc
     7861 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7921 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     7981 gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8041 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8101 ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8221 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8281 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8341 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8401 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8461 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8521 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8581 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8641 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8701 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8761 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8821 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8881 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8941 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9001 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa
     9061 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9181 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9241 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9301 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9361 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9421 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9481 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9541 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9601 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9661 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9721 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9781 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9841 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9901 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9961 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10021 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10081 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10141 tccgcg
//
