LOCUS       sequence-273280-        9892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  *Please note that the depositor recommends using Addgene Plasmid 
            59152 instead.* Lentiviral vector to express p38 KTR mClover under 
            PGK promoter (With Puromycin Resistance)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Kudo T, Jeknic S, Macklin DN, Akhter S, Hughey JJ, Regot S, Covert 
            MW
  TITLE     Live-cell measurements of kinase activity in single cells using 
            translocation reporters.
  JOURNAL   Nat Protoc. 2018 Jan;13(1):155-169. doi: 10.1038/nprot.2017.128. 
            Epub 2017 Dec 21.
  PUBMED    29266096
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.128"; journalName: "Nat Protoc"; date: 
            "2018-01"; volume: "13"; issue: "1"; pages: "155-169"
COMMENT     Sequence Label: pLentiPGK Puro DEST p38KTRClover
FEATURES             Location/Qualifiers
     source          1..9892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1965..2475
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2394..2412
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    2530..2554
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2583..2635
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(3832..3853)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4440..4461
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4497..4521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4552..5140
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4605..4625)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5023..5034)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5154..5653
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5189..5212)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5586..5605
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5674..6273
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5674..5693)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6170..6190
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6411..6644
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6716..6837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6753..6772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6807..6826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6877..7012
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7032..7051)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7033..7051)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7061..7078)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7061..7077)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7070..7092)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7219..7674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7306..7325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7516..7537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7700..7804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7805..7873,7874..8665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8023..8042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8836..9424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9325..9344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9578..9595
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9712..9733
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9748..9753,9754..9771,9772..9778)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9786..9802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9791..9813
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9810..9826
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9810..9826
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9845..9865
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9847..9865
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcacga gactagcctc gagaagcttg atatcgaatt ccacggggtt ggggttgcgc
     1981 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     2041 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     2101 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     2161 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     2221 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     2281 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     2341 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     2401 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     2461 gacctctctc cccaggggga tccaccggtt cgtcgactag tccagtgtgg tggaattctg
     2521 cagatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
     2581 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     2641 ccggtcgcca ccatgtctca ggagaggccc acgttctacc ggcaggagct gaacaagacc
     2701 atctgggagg tgcccgaacg ataccagaac ctgtccccgg tgggctcggg cgcctatggc
     2761 tcggtgtgtg ctgcttttga tacaaagacg gggcatcgtg tggcagttaa gaagctgtcg
     2821 agaccgtttc agtccatcat tcacgccaaa aggacctacc gagagttgcg tctgctgaag
     2881 cacatgaaac acgaaaatgt gattggtctg ttggatgtgt tcacacccgc aaggtcactg
     2941 gaggaattca atgacgtgta cctggtgacc catctcatgg gggcggacct gaacaacatc
     3001 gtgaagtgcc agaagctgac cgacgaccac gttcagtttc tcatctacca gatcctccga
     3061 gggctgaagt atatacattc ggctgacata attcacaggg acctaaagcc cagcaaccta
     3121 gctgtgaacg aagactgtga gctcaagatt ctggattttg ggctggctcg gcacactgat
     3181 gatgagatga caggctacgt ggctaccagg tggtaccgag ccccagagat catgctgaat
     3241 tggatgcact acaaccagac agtggatatt tggtccgtgg gctgcatcat ggctgagctg
     3301 ttgaccggaa gaacgttgtt tcctggtaca gaccatattg atcagttgaa gctcatttta
     3361 agactcgttg gaaccccagg ggctgagctt ctgaagaaaa tctcctcaga gtctgcaaga
     3421 aactacattc agtctctggc ccagatgccg aagatgaact tcgcaaatgt atttattggt
     3481 gccaatcccc tggctgtcga cctactggag aagatgctcg ttttggactc agataagagg
     3541 atcacagcag cccaagctct tgcgcatgcc tactttgctc agtaccacga ccctgatgat
     3601 gagcctgttg ctgaccctta tgaccagtcc tttgaaagca gggaccttct catagatgag
     3661 tggaagagcc tgacctatga tgaagtcatc agctttgtgc caccacccct tgaccaagaa
     3721 gagatggagt cctcaatggg gcatggcacc ggcagcaccg gcagcggcag ctcatccgga
     3781 actagtatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3841 ctggacggcg acgtaaacgg ccacaagttc agcgtccgcg gcgagggcga gggcgatgcc
     3901 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3961 cccaccctcg tgaccacctt cggctacggc gtggcctgct tcagccgcta ccccgaccac
     4021 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4081 atctctttca aggacgacgg tacctacaag acccgcgccg aggtgaagtt cgagggcgac
     4141 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4201 gggcacaagc tggagtacaa cttcaacagc cacaacgtct atatcacggc cgacaagcag
     4261 aagaacggca tcaaggctaa cttcaagatc cgccacaacg ttgaggacgg cagcgtgcag
     4321 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4381 aaccactacc tgagccatca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4441 atggtcctgc tggagttcgt gaccgccgcc taagcggccg cactcgagat atctagaccc
     4501 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct
     4561 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4621 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4681 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4741 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4801 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4861 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4921 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4981 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5041 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5101 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg
     5161 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     5221 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     5281 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     5341 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     5401 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     5461 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     5521 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     5581 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     5641 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     5701 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     5761 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     5821 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     5881 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     5941 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6001 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6061 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6121 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6181 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6241 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     6301 accgaaagga gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac
     6361 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6421 agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6481 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6541 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6601 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6661 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6841 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6901 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6961 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7021 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7081 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7141 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7201 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7261 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7321 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7381 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7441 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7501 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7561 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7621 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7681 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8041 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8341 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8941 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9601 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9661 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9721 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9781 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     9841 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aa
//
