LOCUS       sequence-273286-        7560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Liao RG, Jung J, Tchaicha JH, Wilkerson MD, Sivachenko A, Beauchamp 
            EM, Liu Q, Pugh TJ, Pedamallu CS, Hayes DN, Gray NS, Getz G, Wong 
            KK, Haddad RI, Meyerson M, Hammerman PS
  TITLE     Inhibitor-sensitive FGFR2 and FGFR3 mutations in lung squamous cell 
            carcinoma.
  JOURNAL   Cancer Res. 2013 Jun 20.
  PUBMED    23786770
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2013 Jun 20."
COMMENT     Sequence Label: pBp-FGFR3c-WT
FEATURES             Location/Qualifiers
     source          1..7560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    456..655
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             856..1272
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1202..1224
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1240..1256
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1300..1324
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3743..3767)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3804..3824)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3809..4138
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3989..4124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4148..4747
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4148..4167)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4644..4664
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4968..5437
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5567..5584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5738..6326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5818..5837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6486..7277,7278..7346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7109..7128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7347..7451)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(7482..7560,1..391)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
       61 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
      121 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
      181 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
      241 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
      301 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
      361 tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc
      421 cctgcccagg gaccaccgac ccaccaccgg gaggcaagct ggccagcaac ttatctgtgt
      481 ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta ctagttagct
      541 aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac acccggccgc
      601 aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac ctgaggaagg
      661 gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga cgagaaccta
      721 aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct
      781 tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat
      841 ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt aggtcactgg
      901 aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt
      961 accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt
     1021 aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac
     1081 ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg
     1141 gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc
     1201 ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct
     1261 tctctaggcg ccggccggat cccagtgtgg tggtacatca caagtttgta caaaaaagtt
     1321 ggcatgggcg cccctgcctg cgccctcgcg ctctgcgtgg ccgtggccat cgtggccggc
     1381 gcctcctcgg agtccttggg gacggagcag cgcgtcgtgg ggcgagcggc agaagtcccg
     1441 ggcccagagc ccggccagca ggagcagttg gtcttcggca gcggggatgc tgtggagctg
     1501 agctgtcccc cgcccggggg tggtcccatg gggcccactg tctgggtcaa ggatggcaca
     1561 gggctggtgc cctcggagcg tgtcctggtg gggccccagc ggctgcaggt gctgaatgcc
     1621 tcccacgagg actccggggc ctacagctgc cggcagcggc tcacgcagcg cgtactgtgc
     1681 cacttcagtg tgcgggtgac agacgctcca tcctcgggag atgacgaaga cggggaggac
     1741 gaggctgagg acacaggtgt ggacacaggg gccccttact ggacacggcc cgagcggatg
     1801 gacaagaagc tgctggccgt gccggccgcc aacaccgtcc gcttccgctg cccagccgct
     1861 ggcaacccca ctccctccat ctcctggctg aagaacggca gggagttccg cggcgagcac
     1921 cgcattggag gcatcaagct gcggcatcag cagtggagcc tggtcatgga aagcgtggtg
     1981 ccctcggacc gcggcaacta cacctgcgtc gtggagaaca agtttggcag catccggcag
     2041 acgtacacgc tggacgtgct ggagcgctcc ccgcaccggc ccatcctgca ggcggggctg
     2101 ccggccaacc agacggcggt gctgggcagc gacgtggagt tccactgcaa ggtgtacagt
     2161 gacgcacagc cccacatcca gtggctcaag cacgtggagg tgaatggcag caaggtgggc
     2221 ccggacggca caccctacgt taccgtgctc aagacggcgg gcgctaacac caccgacaag
     2281 gagctagagg ttctctcctt gcacaacgtc acctttgagg acgccgggga gtacacctgc
     2341 ctggcgggca attctattgg gttttctcat cactctgcgt ggctggtggt gctgccagcc
     2401 gaggaggagc tggtggaggc tgacgaggcg ggcagtgtgt atgcaggcat cctcagctac
     2461 ggggtgggct tcttcctgtt catcctggtg gtggcggctg tgacgctctg ccgcctgcgc
     2521 agccccccca agaaaggcct gggctccccc accgtgcaca agatctcccg cttcccgctc
     2581 aagcgacagg tgtccctgga gtccaacgcg tccatgagct ccaacacacc actggtgcgc
     2641 atcgcaaggc tgtcctcagg ggagggcccc acgctggcca atgtctccga gctcgagctg
     2701 cctgccgacc ccaaatggga gctgtctcgg gcccggctga ccctgggcaa gccccttggg
     2761 gagggctgct tcggccaggt ggtcatggcg gaggccatcg gcattgacaa ggaccgggcc
     2821 gccaagcctg tcaccgtagc cgtgaagatg ctgaaagacg atgccactga caaggacctg
     2881 tcggacctgg tgtctgagat ggagatgatg aagatgatcg ggaaacacaa aaacatcatc
     2941 aacctgctgg gcgcctgcac gcagggcggg cccctgtacg tgctggtgga gtacgcggcc
     3001 aagggtaacc tgcgggagtt tctgcgggcg cggcggcccc cgggcctgga ctactccttc
     3061 gacacctgca agccgcccga ggagcagctc accttcaagg acctggtgtc ctgtgcctac
     3121 caggtggccc ggggcatgga gtacttggcc tcccagaagt gcatccacag ggacctggct
     3181 gcccgcaatg tgctggtgac cgaggacaac gtgatgaaga tcgcagactt cgggctggcc
     3241 cgggacgtgc acaacctcga ctactacaag aagacaacca acggccggct gcccgtgaag
     3301 tggatggcgc ctgaggcctt gtttgaccga gtctacactc accagagtga cgtctggtcc
     3361 tttggggtcc tgctctggga gatcttcacg ctggggggct ccccgtaccc cggcatccct
     3421 gtggaggagc tcttcaagct gctgaaggag ggccaccgca tggacaagcc cgccaactgc
     3481 acacacgacc tgtacatgat catgcgggag tgctggcatg ccgcgccctc ccagaggccc
     3541 accttcaagc agctggtgga ggacctggac cgtgtcctta ccgtgacgtc caccgacgag
     3601 tacctggacc tgtcggcgcc tttcgagcag tactccccgg gtggccagga cacccccagc
     3661 tccagctcct caggggacga ctccgtgttt gcccacgacc tgctgccccc ggccccaccc
     3721 agcagtgggg gctcgcggac gtgaccaact ttcttgtaca aagtggtgat gtaggaattc
     3781 gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3841 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3901 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3961 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4021 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4081 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4141 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     4201 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     4261 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     4321 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     4381 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     4441 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     4501 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     4561 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     4621 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     4681 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     4741 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     4801 atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     4861 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     4921 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
     4981 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     5041 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     5101 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     5161 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     5221 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     5281 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     5341 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     5401 tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa
     5461 tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc
     5521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6421 tcacctagat ccttttgcgg ccgcaaatca atctaaagta tatatgagta aacttggtct
     6481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6781 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7081 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7441 ggggttccgc gcacatttcc ccgaaaagtg ccacctgcag cctgaatatg ggccaaacag
     7501 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
//
