LOCUS       sequence-273322-        3469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1104.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1104
FEATURES             Location/Qualifiers
     source          1..3469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     284..303
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     537..554
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    671..692
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(707..712,713..730,731..737)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    745..761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     750..772
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     769..785
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     769..785
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        803..821
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     803..820
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1369..1378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1373..1382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1412..1431)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1413..1431)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1438..1455)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1438..1454)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1447..1469)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1595..2050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1682..1701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1892..1913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2128..2232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2233..2301,2302..3093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2451..2470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3264..3469,1..383)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
       61 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      121 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      181 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      241 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      301 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      361 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      421 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      481 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      541 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      601 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      661 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      721 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      781 atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag ctatgcatcc
      841 aacgcgttgg gagctctccc atatggtcga cctgcaggcg gccgcgaatt cactagtgat
      901 tcagggctaa aagagctgtg gtgtcacaaa agaaatgatt gatgtaggga tcacagagag
      961 gcaaactgct tatcacacag atggatatca gagaatttgt gaagcctatc atgtatggta
     1021 ttactgccag ccagttgcac accttctggg acataatcac agaatatagt aagggattgc
     1081 agattgctac atagcgatca taagccatgg atcccagaag gaaacactca ctgcacacca
     1141 aaccaacaaa gaaatacatc tgaacaaagc agcctacaaa ggatattgat ttctgtgtgg
     1201 actggaaatc aactactgcc ttgggtgtta cagtggagga ataaaatatg tcaatgaatg
     1261 ctaaattgct gaggaaaaaa tacatgggtg tgtgaagctg agagtcaatt ctaattaaca
     1321 agattaaccc aatgttgccc cacacagtga ataaatcgaa ttcccgcggc cgccatggcg
     1381 gccgggagca tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt acaattcact
     1441 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     1501 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     1561 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     1621 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     1681 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     1741 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     1801 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     1861 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     1921 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     1981 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2041 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2221 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2281 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2341 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2401 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2461 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2521 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2581 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2641 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2701 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2761 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2821 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2881 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2941 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3001 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3061 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacat
//
