LOCUS       sequence-273328-        9323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven
            sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque 
            S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, 
            Goulet A, Doyon Y
  TITLE     Versatile and robust genome editing with Streptococcus thermophilus 
            CRISPR1-Cas9.
  JOURNAL   Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub
            2020 Jan 3.
  PUBMED    31900288
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.255414.119"; journalName: "Genome Res"; date: "2020-01";
            volume: "30"; issue: "1"; pages: "107-117"
COMMENT     Sequence Label: U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA
FEATURES             Location/Qualifiers
     source          1..9323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..288
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     40..60
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     211..230
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        460..763
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1041..2057
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1981..2003
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2065..2084
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2160..2180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2181..2864
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2961..6320
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus 
                     thermophilus Type II CRISPR/Cas system (Esvelt et al., 
                     2013)"
                     /label=St1Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="SDLVLGLAIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVR
                     RTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEEL
                     FIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQ
                     LRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKR
                     KYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLLN
                     DLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRIDK
                     SGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADGS
                     FSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKTT
                     SSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNEDD
                     EKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERCL
                     YTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDS
                     MDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTRYASRVVL
                     NALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNLW
                     KKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSYQ
                     VDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSKF
                     LMYRHDPQTFEKVIEPILENYPNKQINEKGKEVPCNPFLKYKEEHGYIRKYSKKGNGPE
                     IKSLKYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQF
                     EKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRTM
                     PKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQH
                     IIKNEGDKPKLDF"
     CDS             6333..6353
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6390..6638
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6669..6917
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6960..6980
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6987..7067
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     polyA_signal    7109..7333
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7406..7423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7577..8165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7657..7676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8336..9127,9128..9196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8959..8978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9197..9301)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgggtcga ctggatccgg taccaaggtc gggcaggaag agggcctatt tcccatgatt
       61 ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
      121 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      181 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      241 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacacccg agacgattaa
      301 tgcgtctcgg tctttgtact ctggtaccag aagctacaaa gataaggctt catgccgaaa
      361 tcaacaccct gtcattttat ggcagggtgt tttttttact agttattaat agtaatcaat
      421 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      481 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      541 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      601 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      661 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      721 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
      781 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
      841 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggsgcgcg ccrggsgggg
      901 sggggggggg gggggggggg gggggggsgr ggsggrgagg kgcggcggca gccaatcaga
      961 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     1021 agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg
     1081 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     1141 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     1201 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     1261 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc
     1321 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc
     1381 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca
     1441 aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg
     1501 ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     1561 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     1621 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     1681 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     1741 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     1801 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     1861 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     1921 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     1981 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     2041 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     2101 gcaaagaatt cagagccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
     2161 caaagaagaa gcggaaagtc tcctcagaga ctgggcctgt cgccgtcgat ccaaccctgc
     2221 gccgccggat tgaacctcac gagtttgaag tgttctttga cccccgggag ctgagaaagg
     2281 agacatgcct gctgtacgag atcaactggg gaggcaggca ctccatctgg aggcacacct
     2341 ctcagaacac aaataagcac gtggaggtga acttcatcga gaagtttacc acagagcggt
     2401 acttctgccc caataccaga tgtagcatca catggtttct gagctggtcc ccttgcggag
     2461 agtgtagcag ggccatcacc gagttcctgt ccagatatcc acacgtgaca ctgtttatct
     2521 acatcgccag gctgtatcac cacgcagacc caaggaatag gcagggcctg cgcgatctga
     2581 tcagctccgg cgtgaccatc cagatcatga cagagcagga gtccggctac tgctggcgga
     2641 acttcgtgaa ttattctcct agcaacgagg cccactggcc taggtaccca cacctgtggg
     2701 tgcgcctgta cgtgctggag ctgtattgca tcatcctggg cctgccccct tgtctgaata
     2761 tcctgcggag aaagcagccc cagctgacct tctttacaat cgccctgcag tcttgtcact
     2821 atcagaggct gccaccccac atcctgtggg ccacaggcct gaagtctgga ggatctagcg
     2881 gaggctcctc tggcagcgag acaccaggaa caagcgagtc agcaacacca gagagcagtg
     2941 gcggcagcag cggcggcagc agcgacctgg tgctgggcct ggctatcggc atcggcagcg
     3001 tgggcgtggg catcctgaac aaggtgaccg gcgagatcat ccacaagaac agtcgcatct
     3061 tccctgctgc tcaggctgag aacaacctgg tgcgccgcac caaccgccag ggtcgccggc
     3121 ttgctcgccg caagaagcac cggcgcgtgc gcctgaaccg cctgttcgag gagagcggcc
     3181 tgatcaccga cttcaccaag atcagcatca acctgaaccc ctaccagctg cgcgtgaagg
     3241 gcctgaccga cgagctgagc aacgaggagc tgttcatcgc cctgaagaac atggtgaagc
     3301 accgcggcat cagctacctg gacgacgcca gcgacgacgg caacagcagc gtgggcgact
     3361 acgcccagat cgtgaaggag aacagcaagc agctggagac caagaccccc ggccagatcc
     3421 agctggagcg ctaccagacc tacggccagc tgcgcggcga cttcaccgtg gagaaggacg
     3481 gcaagaagca ccgcctgatc aacgtgttcc ccaccagcgc ctaccgcagc gaggccctgc
     3541 gcatcctgca gacccagcag gagttcaacc cccagatcac cgacgagttc atcaaccgct
     3601 acctggagat cctgaccggc aagcgcaagt actaccacgg ccccggcaac gagaagagcc
     3661 gcaccgacta cggccgctac cgcaccagcg gcgagaccct ggacaacatc ttcggcatcc
     3721 tgatcggcaa gtgcaccttc taccccgacg agttccgcgc cgccaaggcc agctacaccg
     3781 cccaggagtt caatctgctg aacgacctga acaacctgac cgtgcccacc gagaccaaga
     3841 agctgagcaa ggagcagaag aaccagatca tcaactacgt gaagaacgag aaggctatgg
     3901 gccccgccaa gctgttcaag tacatcgcca agctgctgag ctgcgacgtg gccgacatca
     3961 agggctaccg catcgacaag agcggcaagg ccgagatcca caccttcgag gcctaccgca
     4021 agatgaagac cctggagacc ctggacatcg agcagatgga ccgagagacc ctggacaagc
     4081 tggcctacgt gctgaccctg aacaccgagc gcgagggcat ccaggaggcc ctggagcacg
     4141 agttcgccga cggcagcttc agccagaaac aggtggacga gctggtgcag ttccgcaagg
     4201 ccaacagcag catcttcggc aagggctggc acaacttcag cgtgaagctg atgatggagc
     4261 tgatccccga gctgtacgag accagcgagg agcagatgac catcctgacc cgcctgggca
     4321 agcagaagac caccagcagc agcaacaaga ccaagtacat cgacgagaag ctgctgaccg
     4381 aggagatcta caaccccgtg gtggccaaga gcgtgcgcca ggccatcaag atcgtgaacg
     4441 ccgccatcaa ggagtacggc gacttcgaca acatcgtgat cgagatggcc cgcgagacca
     4501 acgaggacga cgagaagaag gccatccaga agatccagaa ggccaacaag gacgagaagg
     4561 acgccgccat gctgaaggcc gccaaccagt acaacggcaa ggccgagctg ccccacagcg
     4621 tgttccacgg ccacaagcag ctggccacca agatccgcct gtggcaccag cagggcgagc
     4681 gctgcctgta caccggcaag accatcagca tccacgacct gatcaacaac agcaaccagt
     4741 tcgaggtgga ccacatcctg cccctgagca tcaccttcga cgacagcctg gccaacaagg
     4801 tgctggtgta cgccaccgcc aaccaggaga agggccagcg caccccctac caggccctgg
     4861 acagcatgga cgacgcctgg agcttccgcg agctgaaggc cttcgtgcgc gagagcaaga
     4921 ccctgagcaa caagaagaag gagtatctgc tgaccgagga ggacatcagc aagttcgacg
     4981 tgcgcaagaa gttcatcgag cgcaacctgg tggacacccg ctacgccagc cgcgtggtgc
     5041 tgaacgccct gcaggagcac ttccgcgccc acaagatcga caccaaggtg agcgtggtgc
     5101 gcggccagtt caccagccag ctgcgccgcc actggggcat cgagaagacc cgcgacacct
     5161 accaccacca cgccgtggac gccctgatca ttgcggcttc tagccagctg aacctgtgga
     5221 agaagcagaa gaacaccctg gtgagctaca gcgaggacca gctgctggac atcgagaccg
     5281 gcgagctgat cagcgacgac gagtacaagg agagcgtgtt caaggccccc taccagcact
     5341 tcgtggacac cctgaagagc aaggagttcg aggacagcat cctgttcagc taccaggtgg
     5401 acagcaagtt caaccgcaag atcagcgacg ccaccatcta cgccacccgc caggccaagg
     5461 tgggcaagga caaggccgac gagacctacg tgctgggcaa gatcaaggac atctacaccc
     5521 aggacggcta cgacgccttc atgaagatct acaagaagga caagagcaag ttcctgatgt
     5581 accgccacga cccccagacc ttcgagaagg tgatcgagcc catcctggag aactacccca
     5641 acaagcagat caacgagaaa ggcaaggagg tgccctgcaa ccccttcctg aagtacaagg
     5701 aggagcacgg ctacatccgc aagtacagca agaagggcaa cggccccgag atcaagagcc
     5761 tgaagtacta cgacagcaag ctgggcaacc acatcgacat cacccccaag gacagcaaca
     5821 acaaggtggt gctgcagagc gtgagcccct ggcgcgccga cgtgtacttc aacaagacca
     5881 ccggcaagta cgagatcctg gggctgaagt acgccgatct gcagtttgag aaaggcacag
     5941 gcacctacaa gatcagccag gagaagtaca acgacatcaa gaagaaggag ggcgtggaca
     6001 gcgacagcga gttcaagttc accctgtaca agaacgacct tctgctggtg aaggacaccg
     6061 agaccaagga gcaacagctg ttccgcttcc tgagccgcac catgcccaag cagaagcact
     6121 acgtggagct gaagccctac gacaagcaga agttcgaggg cggcgaggcc ctgatcaagg
     6181 tgctgggcaa cgtggccaac agcggccagt gcaagaaggg cctgggcaag agcaacatca
     6241 gcatctacaa ggtgcgcacc gacgtgctgg gcaaccagca catcatcaag aacgagggcg
     6301 acaagcccaa gttggacttc agcagggctg accccaagaa gaagaggaag gtgggatcta
     6361 gcggcgggag cggcgggagc ggggggagca ctaatctgag cgacatcatt gagaaggaga
     6421 ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag gtggaggaag
     6481 tgatcggcaa caagccagag tctgacatcc tggtgcacac cgcctacgac gagtccacag
     6541 atgagaatgt gatgctgctg acctctgacg cccccgagta taagccttgg gccctggtca
     6601 tccaggattc taacggcgag aataagatca agatgctgag cggaggatcc ggaggatctg
     6661 gaggcagcac caacctgtct gacatcatcg agaaggagac aggcaagcag ctggtcatcc
     6721 aggagagcat cctgatgctg cccgaagaag tcgaagaagt gatcggaaac aagcctgaga
     6781 gcgatatcct ggtccatacc gcctacgacg agagtaccga cgaaaatgtg atgctgctga
     6841 catccgacgc cccagagtat aagccctggg ctctggtcat ccaggattcc aacggagaga
     6901 acaaaatcaa aatgctgtct ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc
     6961 ccaagaagaa gaggaaagtc ggatcctacc catacgatgt tccagattac gcttatccct
     7021 acgacgtgcc tgattatgca tacccatatg atgtccccga ctatgcctaa ttcgaaaagt
     7081 gataactcga gtctagaaac cctgcaggct gtgccttcta gttgccagcc atctgttgtt
     7141 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7201 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7261 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7321 gtgggctcta tggagatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8641 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8821 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8881 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8941 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9001 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9061 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9121 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9181 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9241 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9301 gcacatttcc ccgaaaagtg cca
//
