LOCUS       sequence-273332-        5160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5160)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-006   EB3-Sirius//BsaI
FEATURES             Location/Qualifiers
     source          1..5160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2544..2665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2581..2600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2635..2654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3466..3495
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3517..4104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4005..4024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4116..4143)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4167..4958,4959..5027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4790..4809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5028..5130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5131..5160,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tggccgtgaa cgtgtactca accagcgtaa caagcgagaa tctgtcaagg cacgacatgt
      901 tggcttgggt gaacgattcc cttcatctga actacaccaa aatcgaacag ctgtgctctg
      961 gagctgccta ttgccagttt atggacatgc tctttcccgg atgtgtgcac cttcggaaag
     1021 tcaaattcca ggcaaaactg gaacatgagt atatccacaa ctttaaggtt ctgcaagcag
     1081 cttttaagaa gatgggcgtg gacaagatca tcccggtgga aaagctcgtg aaagggaagt
     1141 ttcaggacaa tttcgagttc attcagtggt tcaagaagtt ctttgacgcc aactatgatg
     1201 ggaaagatta caatccactg cttgccagac agggacagga tgtcgcaccg ccacccaatc
     1261 ctggcgacca gatcttcaac aagagcaaga aactgattgg gacagcggtt ccacaacgta
     1321 cttcccctac tggccccaag aacatgcaga cgtctggcag actgagcaat gttgctccac
     1381 cctgtatact ccgcaagaat ccacctagtg ctcgaaatgg cggtcatgag acagatgccc
     1441 agatactgga gctcaaccaa cagctggtag acctgaaact gaccgtcgac ggattggaga
     1501 aagagaggga cttctacttc tccaagttgc gggatataga gctcatttgc caggaacacg
     1561 agagcgaaaa ctctcccgtc atcagtggta tcatcggtat tctgtatgcc actgaagaag
     1621 ggtttgcacc tcctgaagat gacgaaattg aggagcacca acaggaggat caagacgagt
     1681 acctggatgg cgggtcaggc ggcagcgtga gcaaaggaga ggagctcttt acaggagtag
     1741 tcccgatatt ggtggaactt gatggcgacg tcaatggaca ccgtttctcc gtgagtggag
     1801 aaggcgaagg cgatgcaacc tatggtaaac tcaccctgaa gcttatctgc actacgggga
     1861 aactgccagt accttggcca accctggtta caaccctgca gtttggggtt ctgtgttttg
     1921 cccgatatcc cgatcacatg aagcagcatg acttcttcaa gtcagccatg ccagaagggt
     1981 atgtccaaga gaggaccatc ttctttaaag acgatggcaa ctataagacg agagcagaag
     2041 tgaagtttga gggagacact ctggtgaatc ggattgaact caaaggcatc gacttcaagg
     2101 aagatggcaa tatcctgggt cacaaactgg agtacaatgg gatctctagc aacgtgtaca
     2161 tcacagccga taagcagaag aacgggataa aggcgcattt caagattcgc cataacatcg
     2221 aggacggtgg cgttcaactc gctgatcact accagcagaa cactcccatt ggcgatggac
     2281 ctgtgcttct ccctgacaac cactacttgt ctgtccagag caagctgtcc aaagacccca
     2341 atgagaagag ggaccatatg gtcttgctgg agagtgtgac agctgctggg attactctgg
     2401 gtatggacga gctgtacaaa tcaggctctg attaaggcgc caggtacatc tccaatacgg
     2461 cgtcttgcat agagaacacg attagcacac cggttaccca cgtgatgccc gaacggaccc
     2521 atgtcatgcc agagcgcact ggtaacttgt ttattgcagc ttataatggt tacaaataaa
     2581 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2641 tgtccaaact catcaatgta tcttatcatg tctgtcgtcg aattcgagtt ggcgcgcctg
     2701 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     2761 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     2821 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     2881 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     2941 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     3001 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     3061 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     3121 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     3181 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     3241 tccccgtgag ctcccaggcg cgccaactcg gtacctcacc cgattctcat catggagaca
     3301 catcaggaca tctccaatac cgccatggaa acgcaccaag atgcgactgc tcttctgcca
     3361 gtgacggcat tcgtctgcaa gattaacggc atacagaaag aaacgatgag acccattcag
     3421 gacgagcctc agactccagc gtaactggac tgcaatcaac tcactggctc accttcacgg
     3481 gtgggccttt cttcggtaga aaatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3541 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3601 agagctacca actctttttc cgaggtaact ggcttcagca gagcgcagat accaaatact
     3661 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc atcgattttt gtgatgctcg
     4081 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcag aaaggcccac ccgaaggtga
     4141 gccaggtgat tacatttggg ccctcattac caatgcttaa tcagtgaggc acctatctca
     4201 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4261 atgcgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga accacgctca
     4321 ccggctccag atttatcagc aataaaccag ccagccggga gggccgagcg cagaagtgat
     4381 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4441 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4501 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4561 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgccaga
     4621 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4681 gtcatgccat ccgtgagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4741 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4801 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgtaaactc
     4861 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4921 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4981 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatttt agcttcctta
     5041 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     5101 tgaaagttgg aacctcttac gtgccgatca agtcaaaagc ctccggtcgg aggcttttga
//
