LOCUS       sequence-273342-        3472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1349.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1349
FEATURES             Location/Qualifiers
     source          1..3472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..122
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    239..260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(275..280,281..298,299..305)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    313..329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     318..340
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     337..353
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     337..353
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        371..389
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     371..388
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      940..949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      944..953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(983..1002)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(984..1002)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1009..1026)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1009..1025)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1018..1040)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1166..1621
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1253..1272)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1463..1484
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1699..1803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1804..1872,1873..2664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2022..2041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2835..3423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3324..3343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
       61 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      121 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      181 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      241 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      301 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      361 acgccaagct atttaggtga cactatagaa tactcaagct atgcatccaa cgcgttggga
      421 gctctcccat atggtcgacc tgcaggcggc cgcgaattca ctagtgatta agggaaccat
      481 gatgacgagg acggtggcca caaacacatt ggcctcgaaa actctggtgt ctgcacacac
      541 caggctcagc agaggtgcta tgtcacaaaa gaagtgtcgg atgccacgtg gcccacagaa
      601 ggggaagtta aagagccaga tggtgaagac gagtgccaca gggattcccg ccagccagca
      661 ggtagtagct agcaggtagc acagctgcgg gctcatgatg gcgccatagc gcagtggcct
      721 gcagatggcc agaaagcgat cccaggccat gaccatgaga aggaagcact cagatgtgac
      781 acaggacagc acaaggagca gctgcagggc acagctcgtg ggggacaccc cacgtccagg
      841 ctgcagcagg gtcaccagca ggcgggatgc cacgtccagt gagaagcaga tctctaccag
      901 cgccagttgg cgcaggaaga agtaaatcga attcccgcgg ccgccatggc ggccgggagc
      961 atgcgacgtc gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
     1021 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     1081 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     1141 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     1201 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     1261 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     1321 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     1381 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     1441 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     1501 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     1561 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     1621 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc
     1681 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1801 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1861 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2041 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     2281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2341 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2641 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2941 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc tt
//
