LOCUS       sequence-273346-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MCL1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Inde Z, Forcina GC, Denton K, Dixon SJ
  TITLE     Kinetic Heterogeneity of Cancer Cell Fractional Killing.
  JOURNAL   Cell Rep. 2020 Jul 7;32(1):107845. doi: 
            10.1016/j.celrep.2020.107845.
  PUBMED    32640215
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-07-7"; volume: "32(1):107845. doi"; pages: " 
            10.1016/j.celrep.2020.107845"
COMMENT     Sequence Label: pLenti CMV MCL1 Puro
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..44
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     278..295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    412..433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(448..453,454..471,472..478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    486..502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     491..513
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     510..526
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     510..526
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     545..565
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        547..565
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        593..819
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             820..1000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1047..1172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1665..1898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2083..2127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2533..2836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2837..3040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2991..3011
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3037..3061
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3145..3169
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4220..4244)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4275..4863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4328..4348)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4746..4757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4877..5376
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4912..4935)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5309..5328
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5397..5996
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5397..5416)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5893..5913
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6134..6367
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6439..6560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6476..6495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6530..6549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6600..6735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6755..6774)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6756..6774)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6784..6801)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6784..6800)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6793..6815)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6942..7397
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7029..7048)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7239..7260
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7423..7527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7528..7596,7597..8388)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7746..7765)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8559..9023,1..124)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
       61 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      121 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      181 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      241 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      301 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      361 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      421 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      481 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      541 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      601 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      661 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      721 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      781 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     1021 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     1081 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     1141 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1201 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1261 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1321 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1381 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1441 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1501 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1561 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1621 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1681 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1741 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1801 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1861 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1921 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1981 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     2041 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     2101 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     2161 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2221 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2281 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2341 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc
     2521 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2581 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2641 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2701 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2761 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2821 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2881 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2941 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     3001 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     3061 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctagtccag
     3121 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagttggcat gtttggcctc
     3181 aaaagaaacg cggtaatcgg actcaacctc tactgtgggg gggccggctt gggggccggc
     3241 agcggcggcg ccacccgccc gggagggcga cttttggcta cggagaagga ggcctcggcc
     3301 cggcgagaga tagggggagg ggaggccggc gcggtgattg gcggaagcgc cggcgcaagc
     3361 cccccgtcca ccctcacgcc agactcccgg agggtcgcgc ggccgccgcc cattggcgcc
     3421 gaggtccccg acgtcaccgc gacccccgcg aggctgcttt tcttcgcgcc cacccgccgc
     3481 gcggcgccgc ttgaggagat ggaagccccg gccgctgacg ccatcatgtc gcccgaagag
     3541 gagctggacg ggtacgagcc ggagcctctc gggaagcggc cggctgtcct gccgctgctg
     3601 gagttggtcg gggaatctgg taataacacc agtacggacg ggtcactacc ctcgacgccg
     3661 ccgccagcag aggaggagga ggacgagttg taccggcagt cgctggagat tatctctcgg
     3721 taccttcggg agcaggccac cggcgccaag gacacaaagc caatgggcag gtctggggcc
     3781 accagcagga aggcgctgga gaccttacga cgggttgggg atggcgtgca gcgcaaccac
     3841 gagacggcct tccaaggcat gcttcggaaa ctggacatca aaaacgaaga cgatgtgaaa
     3901 tcgttgtctc gagtgatgat ccatgttttc agcgacggcg taacaaactg gggcaggatt
     3961 gtgactctca tttcttttgg tgcctttgtg gctaaacact tgaagaccat aaaccaagaa
     4021 agctgcatcg aaccattagc agaaagtatc acagacgttc tcgtaaggac aaaacgggac
     4081 tggctagtta aacaaagagg ctgggatggg tttgtggagt tcttccatgt agaggaccta
     4141 gaaggtggca tcaggaatgt gctgctggct tttgcaggtg ttgctggagt aggagctggt
     4201 ttggcatatc taataagata gccaactttc ttgtacaaag tggttgatat ccagcacagt
     4261 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4321 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4381 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4441 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4501 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4561 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4621 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4681 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4741 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4801 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4861 cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     4921 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     4981 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     5041 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     5101 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     5161 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     5221 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     5281 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     5341 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     5401 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5461 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5521 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     5581 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     5641 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     5701 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     5761 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     5821 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5881 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5941 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     6001 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
     6061 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6121 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     6181 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6241 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6301 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6361 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6421 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6481 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6541 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     6601 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6661 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6721 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     6781 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6841 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6901 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     6961 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7021 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7081 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7141 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7201 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7261 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     7321 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     7381 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7501 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7561 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7621 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7681 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7741 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7801 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7861 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7921 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7981 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8041 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8101 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8161 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8221 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8281 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8341 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8401 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8461 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8521 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8581 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8641 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8701 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8761 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8821 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8881 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8941 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9001 gtaagcggca gggtcggaac agg
//
