LOCUS       sequence-273352-       16700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducing plant cells to enter mitosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16700)
  AUTHORS   Xu J, Lee YJ, Liu B
  TITLE     Establishment of a mitotic model system by transient expression of 
            the D-type cyclin in differentiated leaf cells of tobacco (Nicotiana
            benthamiana).
  JOURNAL   New Phytol. 2019 Nov 2. doi: 10.1111/nph.16309.
  PUBMED    31679162
REFERENCE   2  (bases 1 to 16700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2019 Nov 2. doi: 10.1111/nph.16309."
COMMENT     Sequence Label: pGWB2-CYCD3;1
FEATURES             Location/Qualifiers
     source          1..16700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5828..5852
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6984..7008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7040..7292
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7721..7973)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8441..9466)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8441..9466)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9476..9820)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9930..9947)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9930..9946)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9939..9961)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10086..10105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10296..10317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10564..10588
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12184..13332)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13631..14425)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14617..15384
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(16007..16700,1..18)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 gttatcaaca agtttgtaca aaaaagcagg cttaatggcg attcggaagg aggaagaaag
     5881 tagagaagaa cagagcaatt cgtttcttct tgatgctctc tactgcgaag aagagaaatg
     5941 ggacgatgaa ggagaagaag ttgaagaaaa ctcttccttg tcttcttctt cttctccatt
     6001 cgttgttttg caacaagatt tgttctggga agatgaagat ctggttacac tcttctccaa
     6061 agaagaagaa caaggactca gctgtctcga tgatgtttat ctttccacgg atcgaaaaga
     6121 agctgttggt tggattctga gagtcaacgc tcattatggc ttctctactt tagcagctgt
     6181 tttagccata acttatctcg ataagttcat ctgtagctac agcttacaga gagacaaacc
     6241 atggatgctt cagctcgttt ctgtcgcgtg tctctcatta gctgctaaag tcgaagaaac
     6301 ccaagtccct cttcttctag actttcaagt ggaggagaca aagtatgtgt ttgaagcaaa
     6361 aaccatacag agaatggagc tactgattct gtctactctc gagtggaaga tgcatctcat
     6421 tactccaatt tcgttcgtag accacattat caggagattg ggacttaaga acaatgctca
     6481 ctgggatttc ctcaacaaat gccaccgtct cctcctctct gtaatctccg attcaagatt
     6541 tgtcgggtac ctcccatcag tagttgccgc agctaccatg atgcgaatta tagagcaagt
     6601 tgatcccttt gaccctcttt cataccaaac taatctcctc ggtgtcctta acttaaccaa
     6661 ggaaaaggtg aaaacttgct acgatctaat cctccaacta ccagtggacc gcatcggttt
     6721 acagatccaa atccaatctt ccaagaaacg caagagtcac gattcatcat catcgttgaa
     6781 cagtccaagc tgcgtgattg atgcaaaccc tttcaatagc gacgaaagct caaacgattc
     6841 gtggtcagcg agttcgtgca acccaccaac gtcgtcgtcg tccccgcagc aacaacctcc
     6901 attgaagaag atgagaggag ctgaagagaa tgagaagaag aagccgattt tgcatctgcc
     6961 atgggcaatc gtagccactc caaacccagc tttcttgtac aaagtggttg ataacagcgc
     7021 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     7081 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     7141 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     7201 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     7261 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat
     7321 gcgcacgacc gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg
     7381 acgtaggcct atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt
     7441 atcagccgcc gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt
     7501 tcacaagaca gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg
     7561 acgaattagt gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac
     7621 ttccaattcg aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc
     7681 tcgattgctc atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac
     7741 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     7801 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     7861 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     7921 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg
     7981 acgcactcct tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac
     8041 gaatcaagtc gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa
     8101 tcttgccttt ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt
     8161 tggcaatagc tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc
     8221 gatgttccat gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt
     8281 attccctcaa agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat
     8341 tgagacgcaa tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga
     8401 cgttgcaaac gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac
     8461 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     8521 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     8581 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     8641 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     8701 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     8761 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     8821 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     8881 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     8941 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     9001 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     9061 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     9121 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     9181 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     9241 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     9301 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     9361 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     9421 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct
     9481 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     9541 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     9601 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     9661 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     9721 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     9781 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     9841 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga
     9901 tctctaggac gcgtcctaga agctaattca ctggccgtcg ttttacaacg tcgtgactgg
     9961 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    10021 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    10081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    10141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    10201 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    10261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    10321 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcgga
    10381 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
    10441 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
    10501 aaaaccaccc cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
    10561 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
    10621 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
    10681 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
    10741 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
    10801 tgacagagcg ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca
    10861 gccttcttat tcatttctcg cttaaccgtg acaggctgtc gatcttgaga actatgccga
    10921 cataatagga aatcgctgga taaagccgct gaggaagctg agtggcgcta tttctttaga
    10981 agtgaacgtt gacgatatca actcccctat ccattgctca ccgaatggta caggtcgggg
    11041 acccgaagtt ccgactgtcg gcctgatgca tccccggctg atcgacccca gatctggggc
    11101 tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa
    11161 ggaagtaggt taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc
    11221 ctgtaggcat cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc
    11281 cggccgccag ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg
    11341 tcagcacggt tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag
    11401 gatctagcgc tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag
    11461 cccccaggac cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga
    11521 ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg
    11581 aaataaacaa caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca
    11641 tccaccagat tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca
    11701 acgcccgcag cagcataccg gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc
    11761 cggacagatg cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc
    11821 caatgatctc gccgtcgatg taggcgccga atgccacggc atctcgcaac cgttcagcga
    11881 acgcctccat gggctttttc tcctcgtgct cgtaaacgga cccgaacatc tctggagctt
    11941 tcttcagggc cgacaatcgg atctcgcgga aatcctgcac gtcggccgct ccaagccgtc
    12001 gaatctgagc cttaatcaca attgtcaatt ttaatcctct gtttatcggc agttcgtaga
    12061 gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg
    12121 ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag
    12181 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
    12241 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
    12301 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
    12361 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
    12421 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
    12481 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
    12541 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
    12601 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
    12661 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
    12721 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
    12781 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
    12841 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
    12901 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
    12961 tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt
    13021 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
    13081 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
    13141 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga
    13201 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
    13261 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
    13321 cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc
    13381 ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat
    13441 gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc
    13501 ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc
    13561 aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca
    13621 catctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
    13681 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
    13741 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
    13801 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
    13861 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
    13921 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
    13981 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
    14041 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
    14101 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
    14161 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
    14221 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
    14281 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
    14341 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
    14401 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
    14461 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    14521 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    14581 agtatcgacg gagccgattt tgaaaccaca attatgggtg atgctgccaa cttactgatt
    14641 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtgtctatca gctgtccctc
    14701 ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta
    14761 tctctgctct cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc
    14821 cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc
    14881 ccgcattatg ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag
    14941 tcggtaacct cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa
    15001 cagtggggct atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt
    15061 ctccggaaga cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt
    15121 cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg
    15181 tatgaatccc gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt
    15241 gagcggcata acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg
    15301 ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac
    15361 tatcaataag ttggagtcat tacccaatta tgatagaatt tacaagctat aaggttattg
    15421 tcctgggttt caagcattag tccatgcaag tttttatgct ttgcccattc tatagatata
    15481 ttgataagcg cgctgcctat gccttgcccc ctgaaatcct tacatacggc gatatcttct
    15541 atataaaaga tatattatct tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc
    15601 atcctcttca tcctcttcgt cttggtagct ttttaaatat ggcgcttcat agagtaattc
    15661 tgtaaaggtc caattctcgt tttcatacct cggtataatc ttacctatca cctcaaatgg
    15721 ttcgctgggt ttatcgcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga
    15781 atgcccaagg taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa
    15841 gtgaagggca ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat
    15901 gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc
    15961 catcccgtta ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg
    16021 gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg
    16081 ggccgggagg gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag
    16141 ggcgcagccc tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac
    16201 aggttagcgg tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt
    16261 ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag
    16321 tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag
    16381 ggcacttatc cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt
    16441 ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc
    16501 atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct
    16561 gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc
    16621 acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc
    16681 ggcgagggca accagcccgg
//
