LOCUS       sequence-273356-       11719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducing plant cells to enter mitosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Xu J, Lee YJ, Liu B
  TITLE     Establishment of a mitotic model system by transient expression of 
            the D-type cyclin in differentiated leaf cells of tobacco (Nicotiana
            benthamiana).
  JOURNAL   New Phytol. 2019 Nov 2. doi: 10.1111/nph.16309.
  PUBMED    31679162
REFERENCE   2  (bases 1 to 11719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2019 Nov 2. doi: 10.1111/nph.16309."
COMMENT     Sequence Label: pGWB660-CYCD3;1
FEATURES             Location/Qualifiers
     source          1..11719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2335..2359)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2370..3083
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      3105..3357
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3391..3407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3391..3407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3404..3426)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3415..3431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3439..3445,3446..3463,3464..3469))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3484..3505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3541..3793)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3953..4504)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4556..4735)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5241..5265
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5786..6577
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6823..7411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7312..7331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7565..7582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7597..7737
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7663..7682)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7823..7845
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8081..8275
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8341..9414)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9843..10472)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gcttaatggc gattcggaag gaggaagaaa gtagagaaga acagagcaat tcgtttcttc
     1261 ttgatgctct ctactgcgaa gaagagaaat gggacgatga aggagaagaa gttgaagaaa
     1321 actcttcctt gtcttcttct tcttctccat tcgttgtttt gcaacaagat ttgttctggg
     1381 aagatgaaga tctggttaca ctcttctcca aagaagaaga acaaggactc agctgtctcg
     1441 atgatgttta tctttccacg gatcgaaaag aagctgttgg ttggattctg agagtcaacg
     1501 ctcattatgg cttctctact ttagcagctg ttttagccat aacttatctc gataagttca
     1561 tctgtagcta cagcttacag agagacaaac catggatgct tcagctcgtt tctgtcgcgt
     1621 gtctctcatt agctgctaaa gtcgaagaaa cccaagtccc tcttcttcta gactttcaag
     1681 tggaggagac aaagtatgtg tttgaagcaa aaaccataca gagaatggag ctactgattc
     1741 tgtctactct cgagtggaag atgcatctca ttactccaat ttcgttcgta gaccacatta
     1801 tcaggagatt gggacttaag aacaatgctc actgggattt cctcaacaaa tgccaccgtc
     1861 tcctcctctc tgtaatctcc gattcaagat ttgtcgggta cctcccatca gtagttgccg
     1921 cagctaccat gatgcgaatt atagagcaag ttgatccctt tgaccctctt tcataccaaa
     1981 ctaatctcct cggtgtcctt aacttaacca aggaaaaggt gaaaacttgc tacgatctaa
     2041 tcctccaact accagtggac cgcatcggtt tacagatcca aatccaatct tccaagaaac
     2101 gcaagagtca cgattcatca tcatcgttga acagtccaag ctgcgtgatt gatgcaaacc
     2161 ctttcaatag cgacgaaagc tcaaacgatt cgtggtcagc gagttcgtgc aacccaccaa
     2221 cgtcgtcgtc gtccccgcag caacaacctc cattgaagaa gatgagagga gctgaagaga
     2281 atgagaagaa gaagccgatt ttgcatctgc catgggcaat cgtagccact ccaaacccag
     2341 ctttcttgta caaagtggtt gataacagca tggtgtctaa gggcgaagag ctgattaagg
     2401 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
     2461 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
     2521 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
     2581 gaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg
     2641 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
     2701 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc
     2761 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc
     2821 tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg
     2881 ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc
     2941 tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca
     3001 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
     3061 aactggggca caaacttaat tgagcttaga gctcgaattt ccccgatcgt tcaaacattt
     3121 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     3181 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     3241 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     3301 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     3361 aattggttcc ggaaccaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3421 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3481 ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct attaattccc
     3541 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
     3601 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     3661 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     3721 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     3781 tgtttgaacg atcggggaaa ttcgagctgg ggacgtcccc agctcggtac cccctcctgg
     3841 gcacggtcgt acgcgatccg ccgcggttcc acctcaccat tgcgggtatg ccagttgagt
     3901 tcgatgagtt cccgtaccag ctccaactcg ggcgatgcca cgcgcactcc gttcagatct
     3961 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     4021 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     4081 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     4141 cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca
     4201 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg
     4261 cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     4321 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     4381 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     4441 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     4501 tcatatctta ttgccccccg ggatcagatc tggattgaga gtgaatatga gactctaatt
     4561 ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta
     4621 gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt
     4681 agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca
     4741 gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt
     4801 aattctccgc tcatgatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     4861 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     4921 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag
     4981 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     5041 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
     5101 agcccttgcg ccctgagtgc ttgcggcagc gtgaagctga tccgtcgaga ttaatagaaa
     5161 taaattcagc ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     5221 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     5281 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     5341 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     5401 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     5461 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
     5521 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     5581 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     5641 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     5701 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     5761 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
     5821 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     5881 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     5941 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     6001 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     6061 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     6121 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     6181 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     6241 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     6301 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     6361 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     6421 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     6481 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     6541 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     6601 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     6661 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     6721 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     6781 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6841 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6901 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6961 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7021 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7081 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7141 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7201 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7261 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7321 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7381 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7441 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7501 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7561 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     7621 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     7681 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     7741 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     7801 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     7861 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     7921 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     7981 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     8041 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     8101 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     8161 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     8221 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     8281 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     8341 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     8401 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     8461 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     8521 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     8581 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     8641 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     8701 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     8761 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     8821 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     8881 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     8941 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     9001 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     9061 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     9121 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     9181 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     9241 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     9301 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     9361 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
     9421 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
     9481 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
     9541 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
     9601 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
     9661 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
     9721 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
     9781 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
     9841 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
     9901 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
     9961 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
    10021 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
    10081 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
    10141 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
    10201 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
    10261 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
    10321 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
    10381 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
    10441 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
    10501 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
    10561 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
    10621 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
    10681 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
    10741 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
    10801 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
    10861 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
    10921 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
    10981 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
    11041 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
    11101 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
    11161 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
    11221 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
    11281 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
    11341 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
    11401 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
    11461 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
    11521 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
    11581 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag
    11641 gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa
    11701 tggacgaacg gataaacct
//
